ID
RTPCR01
Unit Peralatan Makmal Penyelidikan Berpusat
Unit Peralatan Makmal Penyelidikan Berpusat
Real Time PCR System
Brand
BIO-RAD
Model
CFX 384
Intro
Nucleic acid amplification and detection techniques are among the most valuable tools in biological research. RT-PCR is used to detect and quantify RNA. Total RNA is first transcribed into complementary DNA (cDNA).
The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR). In qPCR, the amount of amplification product is measured in each PCR cycle using fluorescence.
Application
● Gene expression analysis
● Genetically testing,
● Cancer phenotyping
● High resolution melt analysis (HRM)
● SNP genotyping
● RNAi validation,
● microarray validation,
● pathogen detection,
● MikroRNAs (miRNAs) quantificatian and profiling
Technical
Specification
● Ramping rates up to 2.5°C/sec
● Temperature range 0 – 100°C
● Multiplex analysis: up to 4 targets per well
● Sample capacity: 384 wells
● High-throughput
Location
Makmal Proteomik & Biologi Molekul, Bangunan Praklinikal
SAMPLE REQUIREMENT
1-30 uL (5-20uL recommended) samples volumes
TYPICAL RESULT
PERSON INCHARGE
Dr. Nur Aqilah Kamaruddin
nur.aqilah@ukm.edu.my
Contact
03-9289 7263
CHARGERS RATE
UKM
RM 20/Day
GOVERNMENT
RM 40/Day
PRIVATE
RM 60/Day