ID
RTPCR03
Unit Peralatan Makmal Penyelidikan Berpusat
Unit Peralatan Makmal Penyelidikan Berpusat
Real Time PCR System
Brand
QIAGEN
Model
ROTOR-GENE Q 5PLEX HRM
Intro
Nucleic acid amplification and detection techniques are among the most valuable tools in biological research. RT-PCR is used to detect and quantify RNA. Total RNA is first transcribed into complementary DNA (cDNA).
The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR). In qPCR, the amount of amplification product is measured in each PCR cycle using fluorescence.
Application
● Gene expression analysis
● Genetically testing,
● Cancer phenotyping
● High resolution melt analysis (HRM)
● SNP genotyping
● RNAi validation,
● microarray validation,
● pathogen detection,
● MikroRNAs (miRNAs) quantificatian and profiling
Technical
Specification
• Optical system: 5 channels plus HRM; Excitation sources: high-energy light-emitting diodes; Detector: photomultiplier; Acquisition time: 4 seconds
• Sample per run: tubes 0.2 ml, 36 samples/run; Strip Tubes 0.1 ml (4 tubes), 72 samples/run; Rotor-Disc 72, 72 samples/run; Rotor-Disc 100, 100 samples/run
• Thermal performance: Temperature range, 35–99°C (95–210.2°F)
• Software: Rotor Gen-Q software
Location
Makmal General 1, Jabatan Mikrobiologi Dan Imunologi Perubatan, Bangunan Praklinikal
SAMPLE REQUIREMENT
1-30 uL (5-20uL recommended) samples volumes *
1- 50 uL (5-200 uL recommended) samples volumes *
* Depends on the rotor usage
TYPICAL RESULT
PERSON INCHARGE
Dr. Nur Aqilah Kamaruddin
nur.aqilah@ukm.edu.my
Contact
03-9289 7263
CHARGERS RATE
UKM
RM 20/Day
GOVERNMENT
RM 40/Day
PRIVATE
RM 60/Day