Sains Malaysiana 33(2): 89-96 (2004)                                                                                                           Sains Hayat /

Life Science

 

 

Kesan Pemberian Ekstrak Buah Mengkudu (Morinda citrifolia Linn.)

 ke atas Kualiti Sperma dan Histologi Testis Mencit

(Effect of Mengkudu (Morinda citrifolia Linn.) Extract on Sperm

Quality and Histology of Testis in Mice)

 

 

Mahanem Mat Noor & Chuay Sin Hsia

Pusat Pengajian BioSains dan Bioteknologi

Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor

 

 

 

ABSTRAK

 

Mengkudu (Morinda citrifolia Linn.) adalah tumbuhan ubatan yang terkenal. Bahagian buah, daun dan akarnya digunakan secara meluas dalam perubatan tradisional. Kajian ini dijalankan untuk menentukan kesan pemberian ekstrak berakua buah M. citrifolia (dos 25mg/kg, 50 mg/kg dan 100 mg/kg berat tubuh mencit selama 30 hari) ke atas kuantiti sperma dan histologi testis mencit. Data daripada kajian ini menunjukkan bilangan sperma epididimis, mortaliti, motiliti dan morfologi sperma mencit kajian tidak berbeza berbanding kumpulan kawalan. Proses spermatogenesis juga tidak terjejas dengan pemberian M. citrifolia. Keputusan kajian ini mencadangkan ekstrak berakua M .citrifolia (sehingga 100 mg/kg) tidak mendatangkan kesan terhadap kualiti sperma dan spermatogenesis mencit.

 

 

ABSTRACT

 

The mengkudu (Morinda citrifolia Linn.) plant is a well known medicinal plant. The fruits, leaves and roots of this plant have been widely used in traditional medicine. The purpose of this study was to evaluate the effects of an aqueous extract of the M. citrifolia fruits (25 mg/kg, 50 mg/kg and 100 mg/kg body weight given for a period of 30 days) on sperm quality and histology of testis in mice. Data from this study show that the epididymal sperm count, mortality, motility and sperm morphology in mice given the M. citrifolia fruit extract did not differ from that of the control group. Spermatogenesis was not affected by the treatment. The results obtained here suggest that the aqueous extract of the M. citrifolia fruit (up to 100 mg/kg) did not affect sperm quality and spermatogenesis in mice.

 

 

RUJUKAN/REFERENCES

 

Bhene, D., Weiler, H. & Kyriakopoulos, A. 1996. Effects of selenium deficiency on testicular morphology and function in rats. Journal of Reproduction and Fertility. 106: 291-297.

Chee, B. J., Noor, H., Umi Kalsom, Y. & Othman, Z. 2000. Towards bridging science and herbal industry: The Hypoglycaemic effect of mengkudu (Morinda citrifolia). Seminar on Medicinal and Aromatic Plants. Forest Research Institute Malaysia (FRIM), Malaysia, 12-13 September.

Hirazumi, A., Furusawa, E., Chou, S.C. & Hokama, Y. 1994. Anticancer activity of Morinda citrifolia (Noni) on intraperitoneally implanted Lewis Lung Carcinoma in syngeneic mice. Proc. West. Pharmacology Society  37 : 145-146.

Humason, G.L. 1979. Animal Tissue Technique (San Francisco, Freeman, W.H. and Company).

Jaganath, I. B. & Ng, L. T. 2000. HERBS The green pharmacy of Malaysia. Malaysia Agricultural Research and Development Institute (MARDI).

Kaul, M. K. & Handa, S.S.2000. Response of medicinal plants to changed habitats and altitudes. Journal of Tropical Medicinal Plants 1: 25-137.

Lohiya, N. K., Pathak, N. Mishra, P. K. & Manivannan, B. 1999. Reversible contraception with chloroform extract of Carica papaya Linn. seed in male rabbits. Reproductive Toxicology. 13(1): 59-66.

Malcolm, S. 1987. Encyclopedia of herbs and herbalism: The biology and chemistry of plants. London: Malcdonald & Co (Publisher Ltd).

Martin, G. B., White, E L.,Markey, E M. & Blackberry, M. A. 1994. Effect of dietary zinc deticiency in the reproduction system of young male sheep: Testicular growth and the secretion of inhibin and testosterone. Journal of Reproduction and Fertility 101: 87-96.

Mohd Zin, Z., Abdul Hamid, A. & Osman A. 2002 Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chemistry 78: 227-231

Moure, A. Cruz, J. M., Franco, D., Dominguez, J. M., Sineiro, J., Dominguez, H., Nunez, M.J. & Parajo, J.E 2001. Natural antioxidants from residual sources. Food Chemistry. 72: 154-171.

Mueller, B. A., Scott, M. K., Sowinki, K. M. & Prag, K. A. 2000. Noni juice (Morinda citrifolia : hidden potential for hyperkalemia. American Journal of Kidney Diseases 35(2): 310-312.

Perry, L. M. 1980. Medicinal plants of east and southeast Asia: Attributed properties and uses. Massachusetts: The M.I.T. Press.

Sanchez-Capelo, A., Castells lis, M. T., Cremades, A. & Penafiel, R. 1996. Hypokalemia decrease testosterone production in male mice by altering luteinizing hormone secretion. Endocrinology 137 (9): 3738-3743.

Schechter, S. 1998. Noni: Island fruit bursting with benefits. Better Nutrition 60(9):30.

Vimala, S. & Adenan, MJ. 1999. Malaysian tropical forest medicinal plants: a source of natural antioxidants. Journal of Tropical Forest Productions 5: 32-38.

Wong, W. Y., Thomas, E.M.G., Merkus, J.M., Zielhuis, G.A. & Steegers-Theunissen, R.P.M. 2000. Male factor subfertility: possible causes and the impact of nutritional factors. Fertility and Sterility 73 (3) : 435-442.

World Health Organization. 1993. Laboratory manual for the examination of human semen and semen-cervical mucus interaction. 3rd Edn. New York: Cambridge University Press.

 

 

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