Sains Malaysiana 35(2): 41-47 (2006)

Cluster Analysis in Lipid Biomarker Studies:

A Case of the Clyde Sea

(Analisis Berkelompok dalam Kajian Penanda

Bio Lipid: Kes di Laut Clyde)



Masni Mohd Ali

School Of Environment And Natural Resource Sciences

Faculty Of Science And Technology

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor, Malaysia


Stephen M. Mudge

School Of Ocean Sciences

University Of Wales, Bangor

Menai Bridge, Anglesey Ll59 5ab

United Kingdom





Tiga puluh tiga sampel sedimen permukaan telah diambil di persekitaran Laut Clyde dan telah dianalisis  bagi menentukan kandungan asid lemak, alkohol lemak dan sterol  dengan menggunakan Kromatografi Gas-Spektrometri Jisim (KGSJ).  Analisis berkelompok (kaedah Ward dengan pemalar korelasi) yang dilakukan secara berasingan ke atas sebatian asid lemak, alkohol lemak dan sterol ini menunjukkan sebatian-sebatian ini dikelompok mengikut sumber geokimia masing-masing. Walaubagaimanapun, dalam setiap analisis, sebatian-sebatian terbitan dari terestrial dapat dilihat berkelompok dengan sebatian-sebatian terbitan marin dan bakteria. Sebatian-sebatian terbitan dari bakteria boleh dijumpai dalam semua kelompok bagi analisis berkelompok yang dijalankan ke atas campuran ketiga-tiga kumpulan sebatian. Ini menunjukkan bahawa input daripada bakteria hadir bersama dengan input daripada marin dan terestrial.


Kata kunci:  analisis berkelompok; penanda bio; asid lemak; alkohol lemak; sterol





Thirty-three surface sediment samples were collected around the Clyde Sea and were analyzed for fatty acids, fatty alcohols and sterols by the GC-MS detection. Cluster analysis (Ward’s method with correlation coefficient) performed separately on fatty acids, fatty alcohols and sterols showed that they were grouped according to their geochemical sources. However, terrestrial derived compounds can be seen grouping with marine and bacterial derived compounds in every analysis. Bacterial derived compounds can be found in all clusters in the mixed compound cluster analysis showing that bacterial inputs occurred together with marine and terrestrial inputs.


Keywords:  cluster analysis; biomarker; fatty acids; fatty alcohols; sterols 





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