Sains Malaysiana 39(3)(2010): 371–376

Nilai Taksonomi Ciri Anatomi Daun Coelostegia Benth. dan Ceiba pentandra

Gaertn. (Bombacaceae)

(Taxonomic Value of Leaf Anatomical Characteristics of Coelostegia Benth. and Ceiba pentandra Gaertn. (Bombacaceae))

S.N. Solihani, T. Noraini* & A.R. Ruzi

Pusat Pengajian Sains Sekitaran dan Sumber Alam, Fakulti Sains dan Teknologi

43600 UKM Bangi, Selangor D.E., Malaysia

R.C.K Chung

Botany and Herbarium Unit, Division Biodiversity and Environment

Forest Research Institute of Malaysia (FRIM)

52109 Kepong, Selangor D.E., Malaysia

Received: 4 May 2009 / Accepted: 14 September 2009

ABSTRAK

Kajian anatomi perbandingan daun dilakukan ke atas tiga spesies daripada genus Coelostegia (C. griffithii, C. borneensis dan C. chartacea) dan juga Ceiba pentandra Gaertn. (Bombacaceae) sebagai spesies perbandingan dalam famili yang sama. Kajian yang dilakukan melibatkan keratan rentas lamina dan tepi daun menggunakan mikrotom gelongsor. Penjernihan daun dilakukan menggunakan larutan 1% Basic Fuchsin di dalam 10% KOH. Hasil kajian menunjukkan ciri sepunya semua spesies Coelostegia yang dikaji iaitu kehadiran sel lendir, jenis trikom, jenis dan taburan hablur, kehadiran sel sklerenkima dan jenis peruratan tengah lamina. Ciri unik yang dikongsi oleh semua spesies genus Coelostegia ialah kehadiran satu hablur tunggal di atas berkas vaskular lamina yang menyambungkannya ke bahagian adaksial epidermis dan seakan-akan membentuk tiang serabut. Manakala variasi interspesies dalam Coelostegia yang boleh digunakan untuk membezakan spesies dalam genus ini ialah bentuk luaran tepi daun, peruratan tepi daun dan taburan trikom. Dalam kajian ini, terdapat tujuh ciri yang boleh membezakan antara Coelostegia dan Ceiba pentandra. Ciri tersebut adalah kehadiran trakeid membengkak yang dapat dilihat pada peruratan lamina, corak berkas vaskular pada tulang daun dan petiol, kehadiran dan jenis hablur, kehadiran dan jenis trikom, sel lendir, sklerenkima pada lamina, hipodermis, sklerenkima pada berkas vaskular petiol dan kehadiran kolenkima. Kajian ini membuktikan bahawa gabungan beberapa ciri anatomi daun mempunyai nilai taksonomi yang boleh digunakan untuk membezakan spesies.

Kata kunci: Anatomi daun; Bombacaceae; Ceiba; Coelostegia

ABSTRACT

A comparative leaf anatomy was undertaken on some Coelostegia species (C.griffithii, C. borneensis and C. chartacea) and also Ceiba pentandra Gaertn. (Bombacaceae) as an outgroup species. The study includes transverse section of laminas and margins using sliding microtome. Leaf clearing was made using Basic Fuchsin in 10 % of KOH. The presence of mucilage cell, types of trichomes, types of laminar venation, presence of schlerenchyma cells and druses are common in all species studied. All Coelostegia species studied, have a unique characteristic in which solitary crystal is present exactly on above vascular bundles touching the adaxial epidermal and forms a structure-like girder. Coelostegia species can be distinguished by the margin outline, types of venation and in the presence or absence of trichomes. This study also showed that there were seven characters that can be used to differentiate between Coelostegia and Ceiba pentandra. The characters were swollen tracheid on the leaf laminar venation, pattern of vascular bundle in the petiole and midrib, the presence and types of crystal, the presence and types of trichomes, mucilagenous canals, hypodermis, collenchyma and schlerenchyma in the medullary vascular bundle in the petiole and in the leaf lamina. This study showed that the combination of anatomical characteristics has taxonomic value that can be useful for distinguishing species.

Keywords: Bombacaceae; Ceiba; Coelostegia; leaf anatomy

REFERENCES

Bose, T.K., Das, P. & Maiti, G.G. 1998. Trees of the World. Jil. 1. India: Regional Plant Resources Centre.

 Chung, R.C.K. 2002. Leaf epidermal micromorphology of Grewia L. and Microcos L. (Tiliaceae) in Peninsular Malaysia and Borneo. Gardens’ Bulletin Singapore 54: 263-286.

Corner, E.J.H. 1988. Wayside Trees of Malaya. Ed. ke-3. Jil. 1. Kuala Lumpur: United Selangor Press.

Florez-Cruz, M., Santana-Lira, H.D. Koch, S.D. & Grether, R. 2004. Taxonomic significance of leaflet anatomy in Mimosa series Quadrivales (Leguminosae, Mimosoideae). Systematic Botany 29(4): 892-902.

Hickey, L.J. 1973. Classification of the architecture of dicotyledons leaves. American Journal of Botany 60: 17-33.

Hinsley, S.R. 2006. Synonymy of Durioneae. http:// www. m a l v a c e a e . i n f o / Synonymy/Synonymy. php?file=DurioneaeMarch.2008

Johansen, D.A. 1940. Plant Microtechnique New York: Mc Graw-Hill Book Company Incorparated.

Khatijah Hussin & Zaharina Mohamat Sani. 1996. The leaf anatomy of Pterygota alata (Roxb.) R. Br., Kleinhovia hospita L. and Commersonia batramia (L.) Merr. (Sterculiaceae). Malaysia Applied Biology 25(1): 41-49.

Khatijah Hussin, Zaharina Mohamat Sani & Fauziah Mohamad Zain. 1997. Comparative leaf anatomical studies of some Heritiera Dryand. (Sterculiaceae). Malaysia Applied Biology 26(1): 7-13.

Khatijah Hussin, Chua, T.S., Halijah Ibrahim, Wu, Q.G., Liao, J.P. & Liu, N. 2000. Comparative Leaf of Alpinia Roxb. Species (Zingiberaceae). Botanical Journal of Linnean Society 133: 161-180.

McLellan, T. 2005. Correlated evolution of leaf shape and trichomes in Begonia dregei (Begoniaceae). American Journal of Botany 2005: 1425-1431.

Metcalfe, C.R. & Chalk, L. 1950. Anatomy of Dicotyledones. Jil. 1. Oxford: Clarendron Press. hlm. 235-242.

Munawirah Pardi, Khatijah Hussin & Latiff, A. 1991 Anatomi petiol beberapa spesies daripada famili Dipterocarpaceae Malaysia, Prosiding Prioriti Penyelidikan 83: 610-617.

Noraini Talip, Khatijah Hussin & Ibrahim, H. 2005. Comparative leaf anatomy of Alpinia species (Zingiberaceae). Nordic Journal of Botany 23: 463-483.

Noraini Talip, Noor Solihani Shamsuddin, Khatijah Haji Hussin & Richard Chung Cheng Kong 2008. Comparative petiol and midrib anatomical characters in selected species of Coelostegia (Bombacaceae) in Malaysia. 3rd Regional Symposium on Environment and Natural Resources, 5-6 August 2008, Kuala Lumpur.

Rao, S.R.S. & Ramayya, N. 1987. Trichome types and their taxonomic importance in the Tiliaceae. Indian Journal Botany 10(1): 65-73. Saas, J.E. 1958. Botanical microtechnique. Ed. ke 3. Calcutta: Oxford & IBH Publishing Company.

Salma, I. 1999. The taxonomic significant of trichome morphology in the Genus Durio (Bombacaceae). Garden’s Bulletin Singapore 51: 55-70

Sidiyasa, K. 2001. Coelostegia montana, a new species of Bombacaceae from Borneo. Journal of Plant Taxonomy and Geography 46(1): 165-168.

Stern, W.L. & Judd, W.S. 2001. Comparative anatomy and systematic of Catasetinae (Orchidaceae). Botanical Journal of Linnean Society 136: 153-178.

*Corresponding author; email: ntalip@ukm.my