Sains Malaysiana 39(3)(2010): 405–412

Profil Aktiviti Laktat Dehidrogenase, Asid Fosfatase Rintang Tartarat dan Alkalin Fosfatase pada Air Liur Semasa Rawatan Ortodontik

(Profiles of Lactate dehydrogenase, Tartrate Resistant Acid Phosphatase and Alkaline Photophatase in Saliva during Orthodontic Treatment)

Shahrul Hisham Zainal Ariffin¹*, Mohd Faiz Ellias¹, Rohaya Megat Abdul Wahab²,

Yosni Bakar³ & Sahidan Senafi¹

¹Pusat Pengajian Biosains dan Bioteknologi Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia 43600 Bangi, Selangor D.E., Malaysia

²Jabatan Ortodontik Fakulti Pergigian Universiti Kebangsaan Malaysia 50300 Kuala Lumpur, Malaysia

³Pusat Pengajian Sains Sekitaran dan Sumber Alam Fakulti Sains dan Teknologi Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, D.E. Malaysia

Received: 14 July 2009 / Accepted: 7 January 2010

ABSTRAK

Proses pergerakan gigi semasa rawatan ortodontik boleh dikelaskan kepada empat fasa iaitu pengaktifan (berkait inflamasi terhadap tisu serta kematian sel), penyerapan, proses berbalik dan pembentukan tulang. Pergerakan gigi ini berkait rapat dengan perubahan metabolik di sekitar mulut. Objektif kajian ini adalah untuk menentukan profil penanda biologi enzim di dalam air liur individu yang menerima rawatan ortodontik iaitu laktat dehidrogenase (LDH) bagi proses inflamasi, asid fosfatase rintang tartarat (TRAP) bagi proses penyerapan tulang dan alkali fosfatas (ALP) bagi proses pembentukan tulang. Sampel air liur diambil daripada 6 individu yang menerima rawatan ortodontik. Aktiviti kesemua enzim diambil sebelum pendakap dipasang (aktiviti normal) diikuti dengan hari ke-3, 7, 10, 14, 17, 21, 24, 28 dan 31 selepas pengaktifan. Hasil kajian mendapati kesemua enzim (LDH, TRAP and ALP) menunjukkan peningkatan yang signifikan (p≤0.05) selepas rawatan diberikan berbanding aktiviti normal. LDH didapati meningkat pada peringkat awal rawatan (hari ke-3,7 dan 10), TRAP pada hari ke 14 dan 17 diikuti dengan ALP pada hari ke-17, 21 dan 24. Sebagai kesimpulan, profil enzim sepanjang rawatan ortodontik menunjukkan proses inflamasi berlaku di peringkat awal rawatan diikuti proses penyerapan dan pembentukan tulang. Selain itu, keseluruhan fasa inflamasi, penyerapan dan pembentukan tulang ortodontik didapati mengambil masa 24 hari.

Kata kunci : Air liur; enzim; ortodontik; pemodelan tulang

ABSTRACT

 During orthodontic treatment, the progress of tooth movements can be classified into four stages, i.e. activation (related to tissue inflammation or cells’ death), resorption, reversal and restructuring of new bones. Tooth movement is related to metabolic changes around the mouth. The objective of this study was to determine enzymes profiles from patient’s saliva during orthodontic tooth movement, i.e., lactate dehydrogenase (LDH) for inflammation, tartrate resistant acid phosphatase (TRAP) for bone resorption and alkaline phosphatase (ALP) for bone formation. Saliva samples were collected from 6 orthodontic patients. Activities of all enzymes were measured before placement of orthodontic fixed appliances (normal activity) followed with day 3, 7, 10, 14, 17, 21, 24, 28 and 31 after the braces were activated. All enzymes (LDH, TRAP and ALP) showed significantly (p≤0.05) higher specific activities as compared to normal. LDH showed the increment at the early phase of treatment (day 3,7 and 10), TRAP at day 14 and 17 followed by ALP at day 17, 21 and 24. As a conclusion, the profiles of enzymes activities showed that inflammation occured during early phase of treatment followed by bone resorption and bone formation. In addition, the inflammations, resorption and bone formation phases in orthodontic treatment were found to be completed within 24 days.

Keywords : Bone remodeling; enzymes; orthodontic; air liur 

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*Corresponding author; email: hisham@ukm.my