Sains Malaysiana 41(11)(2012): 1315–1324


Pemencilan Yis daripada Buah-buahan Tempatan dan Rebung (Bambusa vulgaris)

dan Potensinya sebagai Agen Penaik Roti Putih

(Potential of Yeasts Isolated from Local(Fruits and Bamboo Shoots (Bambusa vulgaris)

as Leavening Agent in White Bread)


A.G. Ma’aruf*, F.Y. Chung & Z. Noroul Asyikeen

Food Science Program, School of Chemical Sciences and Food Technology

Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi,

Selangor D.E. Malaysia


Received: 09 September 2011 / Accepted:  27 June 2012



Kajian ini dijalankan untuk menentukan keupayaan yis (Saccharomyces cerevisiae) dipencil daripada buah-buahan tempatan iaitu duku langsat (Lansium domesticum), rambutan (Nephelium lappaceum ), mangga Chokanan (Mangifera indica  cv. Chokanan) dan rebung buluh minyak (Bambusa vulgaris) sebagai agen penaik roti berbanding roti kawalan penambahan yis komersial. Isi padu tertentu roti kawalan menunjukkan perbezaan bererti (p<0.05) dengan semua roti kajian. Roti yang difermentasi oleh yis rebung buluh menunjukkan isi padu tertentu yang paling tinggi, diikuti oleh roti yang difermentasi oleh yis mangga dan yis duku langsat dan ketiga-tiga yis tersebut juga mempunyai isi padu tertentu yang lebih tinggi secara bererti (p<0.05) dengan roti kawalan. Roti yang difermentasi oleh yis rebung buluh dan yis mangga mempunyai tekstur yang lebih lembut berbanding roti kawalan. Peningkatan dalam isi padu tertentu roti boleh meningkatkan kelembutan tekstur roti. Peratus kandungan kelembapan kulit dan isi roti yang difermentasi oleh yis rebung buluh adalah paling tinggi manakala roti yang difermentasi oleh yis rambutan menunjukkan peratus kandungan kelembapan yang paling rendah. Peningkatan peratus kandungan kelembapan juga boleh meningkatkan kelembutan tesktur roti dan sebaliknya. Daripada segi warna kulit, hanya kecerahan warna kulit (L*) roti yang difermentasi oleh yis rebung buluh tidak menunjukkan perbezaan bererti (p>0.05) dengan warna kulit roti kawalan. Kecerahan warna isi (L*) roti kawalan pula menunjukkan perbezaan bererti (p<0.05) dengan semua isi roti kajian. Selain itu, didapati semakin kecil dan padat liang udara, semakin putih warna isi roti. Keseragaman taburan liang udara juga menghasilkan isi roti yang lebih putih. Secara keseluruhan, kesemua yis yang dipencil berpotensi untuk dijadikan sebagai agen penaik. Yis rebung buluh dan yis mangga dapat menghasilkan kualiti roti putih yang lebih baik daripada yis komersial.


Kata kunci: Duku langsat; mangga; rambutan; rebung; roti; yis



The study was carried out to determine the leavening ability of the yeast (Saccharomyces cerevisiae) isolated from local fruits which were duku langsat(Lansium domesticum), rambutan (Nephelium lappaceum), mango chokanan (Mangifera indica cv. Chokanan) and bamboo shoot (Bambusa vulgaris) in breads, compared with commercial yeast. Specific volume of control bread showed significant difference (p<0.05) with all the bread tested. Bread that was fermented by bamboo shoot’s yeast showed the highest specific volume, followed by bread that was fermented by mango’s yeast and duku langsat’s yeast, and that all three yeasts have specific volume that were significantly higher (p<0.05) than the control bread. Breads that were fermented by bamboo shoot’s yeast and mango’s yeast have texture that was softer compared to the control bread. Increase in the specific volume can improve the bread texture softness. Moisture content of crust and crumb that were fermented by bamboo shoot’s yeast were the highest while bread that was fermented by rambutan’s yeast has the lowest moisture content. Increase in percentage of moisture content could also enhance the bread softness. For crust color, only brightness (L*) of crust bread that was fermented by bamboo shoot’s yeast did not showed significant difference (p>0.05) with crust color of control bread. Meanwhile, brightness (L*) of crumb of control bread showed significant difference (p<0.05) with all of the studied bread crumb. Apart from that, it was also found that the smaller and more compact the pore size, the whiter the bread crumb color will be. Uniform distribution of pore also produced whither bread crumb. Overall, all isolated yeasts can be potentially used as leavening agents. Bamboo shoot’s yeast and mango’s yeast can produce better bread qualities compared with commercial yeast.


Keywords: Bamboo shoot; duku langsat; mango; rambutan; yeast


Al-Eid, S.M., Al-jasass, F.M. & Hamad, S.H. 2010. Performance of baker’s yeast produced using date syrup substrate on Arabic bread quality. African Journal of Biotechnology 9(21): 3167-3174.

Barcenas, M.E. & Rosell, C.M. 2005. Effect of HPMC on the microstructure, quality and aging of wheat bread. Food Hydrocolloids 19: 1037-1043.

Berglund, P., Shelton, D. & Freeman, T. 1991. Frozen bread dough ultra structure as affected by duration of frozen storage and freeze thaw cycles. Cereal Chem.  68: 105-107.

Boboye, B. & Dayo-Owoyemi, I. 2009. Evaluation of dough sensory properties impacted by yeasts isolated from cassava. Journal of Applied Sciences 9(4): 771-776.

Boboye, B., Dayo-Owoyemi, I. & Akinyosoye, F.A. 2008. Organoleptic analysis of doughs fermented with yeasts from a Nigerian Palm wine (Elaeis guineensis) and certain commercial yeasts. Open Microbiology Journal 2: 115-119.

Burhans, M.E. & Clapp, J. 1942. A microscopic study of bread and dough. Cereal Chemistry 19: 196-216.

Cauvain, S.P. 2001. Breadmaking. In Cereals Processing Technology, edited by Owens, G. Cambridge: Woodhead Publishing Ltd.

Cauvain, S.P., Whitworth, M.B. & Alava, J.M. 1999. The evolution of bubble structure in bread doughs and its effect on bread structure. In Bubbles in Food, edited by Campbell, G.M., Webb, C., Pandiella, S.S. & Niranjan, K., St. Paul, MN: Eagan Press.

Cauvain, S.P. & Young, L.S. 2000. Bakery Food Manufacture and Quality: Water Control and Effects. United Kingdom: Blackwell Science.

Charoenthaikij, P., Jangchud, K., Jangchud, A., Prinyawiwatkul, W., No, H.K. & King, J.M. 2010. Physicochemical properties and consumer acceptance of wheat-germinated brown rice bread during storage time. Journal of Food Science 75: S333-S339.

Chevallier, S., Zúñiga, R. & Le-Bail, A. 2010. Assessment of bread dough expansion during fermentation. Food and Bioprocess Technology: 1-9.

Dimitrellou, D., Kandylis, P., Kourkoutas, Y., Koutinas, A.A. & Kanellaki, M. 2009. Evaluation of thermally-dried Kluyveromyces marxianus as baker’s yeast. Food Chemistry 115(2): 691-696.

Hathorn, C.S., Biswas, M.A., Gichuhi, P.N. & Bovell-Benjamin, A.C. 2008. Comparison of chemical, physical, micro-structural, and microbial properties of breads supplemented with sweet potato flour and high-gluten dough enhancers. LWT - Food Science and Technology 41(5): 803-815.

Hirasawa, R. & Yokoigawa, K. 2001. Leavening ability of baker’s yeast exposed to hyperosmotic media. FEMS Microbiology Letters 194:159-162.

Ishida, N., Takano, H., Naito, S., Isobe, S., Uemura, K., Haisi, T., Kose, K., Koizumi, M. & Kano, H. 2001. Architecture of baked breads depicted by a magnetic resonance imaging. Magnetic Resonance Imaging 19(6): 867-874.

Kamman, P.W. 1970. Factors affecting the grain and texture of white bread. Bakers Digest 44(2): 34-38.

Kruger, J.E. & Reed, G. 1988. Enzymes and color. In Wheat Chemistry and Technology, edited by Pomeranz, Y. St. Paul, Mn: American Association of Cereal Chemists.

Loo, Chee Yeong. 2009. Penyaringan dan pengoptimuman penghasilan protein intrasel yis dalam medium sisa pertanian untuk industi makanan. Tesis Sarjana. Universiti Sains Malaysia (tidak diterbitkan).

Maaruf, A.G. Noroul Asyikeen, Z. Sahilah, A.M. and Mohd. Khan, A. 2011. Leavening ability of yeast isolated from different local fruits in bakery product. Sains Malaysiana 40(12): 1413-1419.

Olorunfemi, O.B. & Adetuyi, F.C. 2005. Isolation of baking yeast from naturally fermented pineapple. Journal of Food, Agriculture & Environment 3: 115-117.

Patel, B.K., Waniska, R.D. & Seetharaman, K. 2005. Impact of different baking processes on bread firmness and starch properties in breadcrumb. Journal of Cereal Science 42: 173-184.

Plessas, S., Pherson, L., Bekatou, A., Nigam, P. & Koutinas, A. A. 2005. Bread making using kefir grains as baker’s yeast. Food Chemistry 93: 585-589 .

Poinot, P., Arvisent, G., Grua-Priol, J., Colas, D., Filllonneau, C., Le Bail, A. & Prost, C. 409, 2008. Influence of formulation and process on the aromatic profile and physical 410 characteristics of bread. Journal of Cereal Science 48: 686-697.

Pyler, E. J. 1988. Baking Science and Technology. Merriam, KS: Sosland Publishing Co.

Rogers, D.E., Zeleznak, K.J., Itai, C.S. & Hoseney, R.C. 1988. Effect of native lipids, shortening and bread moisture on bread firming. Cereal Chem. 65: 398-401.

Sahin, S. & Sumnu, S.G. 2006. Physical Properties of Foods. New York: Springer Science+Business Media.

Salovaara, H. & Valjakka, T. 1987.  The effect of fermentation temperature, flour type and starter on the sour wheat bread. International Journal of Food Science & Technology 22(6): 591-597.

Selomulyo, V.O. & Zhou, W.B. 2007. Frozen bread dough: Effects of freezing storage and dough improvers. Journal of Cereal Science 45: 1-17.

Somiari, R.I & Udoh, A.E. 1993. Evaluation of the performance of yeast isolated from the sap of Elaesis guineansisin dough leavening. Nigerian Food Journal 2: 32-44.

Stringini, M., Comitini, F., Taccari, M. & Ciani, M. 2008. Yeast diversity in crop-growing environments in Cameroon. International Journal of Food Microbiology 127: 184-189.

Tamang, J.P. & Fleet, G.H. 2009. Yeasts diversity in fermented foods and beverages. In Yeast Biotechnology: Diversity and Applications, edited by Satyanarayana, T. & Kunze, G. New York: Springer Science Business Media B.V.

Tong, Q., Zhang, X., Wu, F., Tong, J., Zhang, P. & Zhang, J. 2010. Effect of honey powder on dough rheology and bread quality. Food Research International 43(9): 2284-2288.

Trindade, R.C., Resende, M.A., Silva, C.M. & Rosa, C.A. 2002. Yeasts associated with fresh and frozen pulps of Brazilian tropical fruits. Systematic and Applied Microbiology 25(2): 294-300.

van Vliet, T., Janssen, A.M., Bloksma, A.H. & Walstra, P. 1992. Strain hardening of dough as a requirement for gas retention. Journal of Texture Studies 23: 439-460.

Wang, J. & Coles, G.D. 1994. Objective measurement of bread crumb texture. SPIE Proceedings, Optics in Agriculture, Forestry, and Biological Processing, pp. 2345: 85-94.

Wongkhalaung, C. & Boonyaratanakornkit, M. 2007. Characterization of new baker’s yeast strains and their leavening ability in bread dough. Kasetsart Journal - Natural Science 41(4): 751-763.

Yi, J.H. & Kerr, W.L. 2009. Combined effects of dough freezing and storage conditions on bread quality factors. Journal of Food Engineering 93: 495-501.

Zayas, I.Y. 1993. Digital image texture analysis for bread crumb grain evaluation. Cereal Foods World 38:760-766.



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