Sains Malaysiana 44(3)(2015): 355–363


Comparison of Phytochemicals and Antioxidant Properties of Different Fruit Parts of Selected Artocarpus species from Sabah, Malaysia

(Perbandingan Ciri Fitokimia dan Antioksida pada Bahagian Buah yang Berbeza

bagi Spesies Artocarpus Terpilih dari Sabah, Malaysia)





1Food Technology Panel, Department of Technology & Heritage, Faculty of Science,

Technology and Human Development, Universiti Tun Hussein Onn Malaysia (UTHM)

86400 Parit Raja, Batu Pahat, Johor, Malaysia


2Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS

88400 Kota Kinabalu, Sabah, Malaysia


Received: 4 February 2014/Accepted: 10 September 2014



The purpose of this study is to investigate and compare the phytochemical contents and antioxidant activity of 80% methanol extracts of three selected fruits of Artocarpus species namely, Artocarpus odoratissimus (tarap), Artocarpus kemando (pudu) and Artocarpus integer (cempedak). The total phenolic, total flavonoid and total carotenoid contents of different parts of the fruits (peel, flesh and seed) were analyzed spectrophotometrically. The antioxidant properties were assessed by DPPH, FRAP and ABTS method. The total phenolic content of all parts of the fruits ranging from 3.53 to 42.38 mg GAE/g of dry sample. The total flavonoid was in the range of 0.82 to 36.78 mg CE/g of dry sample whereas the total carotenoid ranging from 0.67 to 3.30 mg ß-carotene/g of dry sample. The peel and seed displayed higher phytochemical contents (as compared with the flesh) and were found to be efficient radical scavengers and reducing agents. Total phenolic and total flavonoid contents were significantly correlated with the antioxidant activities. However, the total carotenoid was weakly correlated with the antioxidant activities. Due to the findings of this research, it is observed that the phytochemical compounds are the major contributor to the antioxidant activities. A. odoratissimus has the potential to be used as antioxidant agents due to high phytochemical contents and high antioxidant activity for all tested methods.


Keywords: Antioxidant activity; Artocarpus species; phytochemical contents



Tujuan penyelidikan ini dijalankan adalah untuk mengkaji dan membuat perbandingan kandungan fitokimia dan aktiviti antioksida pada ekstrak metanol 80% bagi tiga spesies buah Artocarpus yang terpilih iaitu Artocarpus odoratissimus (tarap), Artocarpus kemando (pudu) dan Artocarpus integer (cempedak). Jumlah kandungan fenolik, flavonoid dan karotenoid bahagian buah (isi, kulit dan biji) telah dianalisis dengan menggunakan spektrofotometri. Aktiviti antioksida ditentukan melalui kaedah DPPH, FRAP dan ABTS. Kesemua bahagian sampel menunjukkan jumlah kandungan fenolik berjulat daripada 3.53 hingga 42.38 mg GAE/g sampel kering. Jumlah kandungan flavonoid pula berjulat daripada 0.82 hingga 36.78 mg CE/g sampel kering manakala jumlah kandungan karotenoid berjulat dari 0.67 hingga 3.30 mg ß-karotene/g sampel kering. Bahagian kulit dan biji menunjukkan kandungan fitokimia yang tinggi (berbanding isi) dan merupakan penghapus radikal dan agen penurunan yang cekap. Jumlah kandungan fenolik dan flavonoid berkorelasi secara signifikan dengan aktiviti antioksida. Walau bagaimanapun, jumlah kandungan karotenoid berkorelasi secara lemah dengan aktiviti antioksida. Berdasarkan hasil kajian, kompaun fitokimia merupakan penyumbang utama kepada aktiviti antioksida. A. odoratissimus berpotensi untuk dijadikan agen antioksida disebabkan oleh kandungan fitokimia dan aktiviti antioksida yang tinggi terhadap semua kaedah yang dijalankan.


Kata kunci: Aktiviti antioksida; kandungan fitokimia; spesies Artocarpus


Abu Bakar, M.F., Mohamad, M., Rahmat, A., Burr, S.A. & Fry, J.R. 2010. Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan). Food and Chemical Toxicology 48: 1688-1697.

Abu Bakar, M.F., Mohamed, M., Rahmat, A. & Fry, J.R. 2009. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chemistry 113: 479-483.

Andarwulan, N., Fardiaz, D., Wattimena, G.A. & Shetty, K. 1999. Antioxidant activity associated with lipid and phenolic mobilization during seed germination of Pangium edule Reinw. Journal of Agricultural and Food Chemistry 47: 3158-3163.

Benzie, I.F. & Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP Assay. Analytical Biochemistry 239: 70-76.

Chong, K.Y., Tan, H.T.W. & Corlett, R.T. 2009. A Checklist of the Total Vascular Plant Flora of Singapore: Native, Naturalised and Cultivated Species. Singapore: Raffles Museum of Biodiversity Research, National University of Singapore, Singapore.

Dueñas, M., Hernández, T., Estrella, I. & Fernández, D. 2009. Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.). Food Chemistry 117: 599-607.

Ee, G.C.L., Teo, S.H., Lim, C.K., Lim, Y.M. & Boong, C.F.J. 2012. Free radical scavenging effect of Artocarpus kemando and Artocarpus odoratissimus: Structure-activity relationship of flavonoid derivatives. Asian Journal of Chemistry 24(1): 231-234.

Ee, G.C.L., Teo, S.H., Rahmani, M., Lim, Y.M. & Boong, C.F.J. 2010. Artosimmin-a potential anti-cancer lead compound from Artocarpus odoratissimus. Letters in Organic Chemistry 7(3): 240-244.

Hakim, E.H., Juliawaty, L.D., Syah, Y.M. & Achmad, S.A. 2005. Molecular diversity of Artocarpus integer (Moraceae): A species endemic to Indonesia. Molecular Diversity 9: 149- 158.

Hashim, N.M., Rahmani, M., Shamaun, S.S., Ee, G.C.L., Sukari, M.A., Ali, A.M. & Go, R. 2011. Dipeptide and xanthones from Artocarpus kemando Miq. Journal of Medicinal Plant Research 5(17): 4224-4230.

Heim, K.E., Tagliafero, A.R. & Bobilya, D.J. 2002. Flavonoid antioxidants: Chemistry metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry 13: 572-584.

Hess, D., Keller, H.E., Oberlin, B., Bonfanti, R. & Schüep, W. 1991. Simultaneous determination of retinol, tocopherols, carotenes and lycopene in plasma by higher performance liquid chromatography on reversed phase. International Journal of Vitamin and Nutrition Research 61(3): 232-238.

Howitt, C.A. & Pogson, B.J. 2006. Carotenoid accumulation and function in seeds and non-green tissues. Plant, Cell and Environment 29(3): 435-445.

Jagtap, U.B. & Bapat, V.A. 2010. Artocarpus: A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology 129: 142-166.

Kubola, J. & Siriamompun, S. 2011. Phytochemicals and antioxidant activity of different fruits fractions (peel, pulp, aril and seed) of Thai gac (Momordica cochinchinensis Spreng). Food Chemistry 127: 1138-1145.

Ladislav, F., Vera, P., Karel, S. & Karel, V. 2005. Reliability of carotenoid analyses: A review. Current Analytical Chemistry 1: 93-102.

Liu, R.H. 2004. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. Journal of Nutrition 134(12): 3479-3485.

Mensor, L.I., Menezes, F.S., Leitao, G.G., Reis, A.S., Santos, T.D., Coube, C.S. & Leitao, S.G. 2001. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotheraphy Research 15: 127-130.

Osorio-Esquivel, O., Moreno, A.O. & Alvarez, V.B. 2011. Phenolics, betacyanins and antioxidant activity in Opuntia jaconostle fruits. Food Research International 44: 2160- 2168.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radical Biology & Medicine 26: 231-1237.

Remorini, D., Tavarini, S., Degl'Innocenti, E., Loreti, F., Massai, R. & Guidi, L. 2008. Effects of rootstocks and harvesting time on the nutritional quality of peel and flesh of peach fruits. Food Chemistry 110(2): 361-367.

Rice-Evans, C.A., Miller, N.J. & Paganga, G. 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science 2(4): 152-159.

Soepadmo, E. & Saw, L.G. 2000. Tree Flora of Sabah and Sarawak. Vol 3. Malaysia: Government of Malaysia.

Soong, Y. & Barlow, P.J. 2004. Antioxidant activity and phenolic content of selected fruit seeds. Food Chemistry 88: 411-417.

Spitaler, R., Schlorhaufer, P.D., Ellmerer, E.P., Merfort, I., Bortenschlager, S., Stuppner, H. & Zidorn, C. 2006. Altitudinal variation of secondary metabolites profiles in flowering heads of Arnica Montana cv. ARBO. Phytochemistry 67: 409-417.

Syah, Y.M., Juliawaty, L.D., Achmad, A.S., Hakim, E.H. & Ghisalberti, E.L. 2006. Cytotoxic prenylated flavones from Artocarpus integer. Journal of Natural Medicines 60: 308- 312.

Velioglu, Y.S., Mazza, G., Gao, L. & Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. Journal of Agricultural and Food Chemistry 46: 4113-4117.

Wang, W.J., Li, X.Y. & Zu, Y.G. 2005. Dynamic feature of flavonoid content in different organs of larch (Larix gmelinii). Journal of Forestry Research 16(2): 89-92.

Zhishen, J., Mencheng, T. & Jianming, W. 1999. The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chemistry 64: 555-559.



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