Sains Malaysiana 45(1)(2016): 115–118

Studies on Separation and Properties of Lumbrokinase in Pheretima praepinguis

(Kajian ke atas Pemisahan dan Sifat Lumbrokinase dalam Pheretima praepinguis)

TANG MEI, LIU CAO, LIANG ZI, GONG MINGFU* & HU DIE

College of Life Science, Leshan Normal University, Leshan, Sichuan 614000, China

Received: 23 July 2014/Accepted: 21 November 2014

ABSTRACT

In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lumbrokinase activity was studied. The activity of lumbrokinase separated from Pheretima praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60° and wide range of temperature adaptation.

Keywords: Casein medium plate method; Lumbrokinase; Pheretima praepinguis; salting out method; separation

ABSTRAK

Pengasingan lumbrokinase dalam Pheretima praepinguis dan untuk mengkaji sifat enzim, dengan Pheretima praepinguis sebagai bahan, lumbrokinase telah dipisahkan dengan kaedah penggaraman keluar. Aktiviti lumbrokinase diukur dengan kaedah kasein plat sederhana. Kesan pH dan suhu aktiviti lumbrokinase telah dikaji. Aktiviti lumbrokinase dipisahkan dari Pheretima praepinguis adalah agak tinggi. Aktiviti Lumbrokinase dalam persekitaran neutral atau sedikit alkali adalah lebih tinggi. Lumbrokinase mempunyai keupayaan toleransi kepada suhu tinggi, dengan aktiviti yang sangat enzim bawah 60° dan pelbagai penyesuaian suhu.

Kata kunci: Kaedah kasein plat sederhana; kaedah penggaraman keluar; lumbrokinase; pengasingan; Pheretima praepinguis

REFERENCES

Ashraf, M.A., Ullah, S., Ahmad, I., Qureshi, A.K., Balkhair, K.S. & Rehman, M.A. 2013. Green biocides, a promising technology: Current and future applications. Journal of the Science of Food and Agriculture 94(3): 388-403.

Astrup, T. & Mullertz, S. 1952. The fibrin plate method for estimating fibrinolytic activity. Archives of Biochemistry and Biophysics 40: 346-351.

Batool, S., Khalid, A., Chowdury, A.J.K., Sarfraz, M., Balkhair, K.S. & Ashraf, M.A. 2015. Impacts of azo dye on ammonium oxidation process and ammonia oxidizing soil bacteria. RSC Advances 5: 34812-34820.

Butt, M.A., Ahmad, M., Fatima, A., Sultana, S., Zafar, M., Yaseen, G., Ashraf, M.A., Shinwari, Z.K. & Kayani, S. 2015. Ethnomedicinal uses of plants for the treatment of snake and scorpion bite in Northern Pakistan. Journal of Ethnopharmacology 1: 1-14.

Chi, Y.J., Tian, B. & Zhang, Y.H. 1999. Studies on properties and thrombolytic activity of fibrinolytic enzyme from earthworm. Journal of Northeast Agricultural University 30(4): 267-270.

Chu, W.P., Fu, Q. & Zhang, M.H. 2008. The effect of lumbrokinase treatment on hemorheology, glucose and lipids in type 2 diabetes mellitus. Chinese Journal of Diabetes 16(8): 489-490.

Hang, R. 2009. Observation of 100 treatment cases of diabetes combined microcirculation with lumbrokinase. China Practice Medicine 20(4): 153-154.

Khaskheli, A.A., Talpur, F.N., Ashraf, M.A., Cebeci, A., Jawaid, S. & Afridi, H.I. 2015. Monitoring the Rhizopus oryzae lipase catalyzed hydrolysis of castor oil by ATR-FTIR spectroscopy. Journal of Molecular Catalysis B: Enzymatic 113: 56-61.

Kiyani, S., Ahmad, M., Zafar, M.A., Sultana, S., Khan, M.P.Z., Ashraf, M.A., Hussain, J. & Yaseen, G. 2014. Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies-Abbottabad, Northern Pakistan. Journal of Ethnopharmacology 156: 47-60.

Li, Z.Y. 2009. Efficacy of Ischemic stroke prevention with lumbrokinase capsules. Capital Medicine 16: 58.

Li, Y.L. 1957. Large earthworms in Mount Emei. Bulletin of Biology (5): 20-22.

Lu, L., Xu, Y.N., Liu, J., Qian, M. & Wang, J. 2007. Clinical efficacy of injectable lumbrokinase. Acta Universitatis Medicinalis Nanjing (Natural Science) 27(11): 1326-1327.

Lukman, A.L., Nordin, N.F.H. & Kamarudin, K.R. 2014. Microbial population in the coelomic fluid of Stichopus chloronotus and Holothuria (Mertensiothuria) leucospilota collected from Malaysian waters. Sains Malaysiana 43(7): 1013-1021.

Mihara, H., Sumi, H. & Akazawa, T. 1983. Fibrinolytic enzyme extracted from the earthworm. Thromb Haemost 50: 258-263.

Naureen, R., Tariq, M., Yusoff, I., Choudhury, A.J.K. & Ashraf, M.A. 2014. Synthesis, spectroscopic and chromatographic studies of sunflower oil biodiesel using optimized base catalyzed methanolysis. Saudi Journal of Biological Sciences 22: 322-339.

Qian, C.M., Dang, H.L. & Jing, X. 2005. Drug analysis of treatment of lacunar brain and cerebral arteriosclerosis. Journal of Northwest Pharmaceutical 20(5): 234-235.

Surhio, M.A., Talpur, F.N., Nizamani, S.M., Amin, F., Bong, C.W., Lee, C.W., Ashraf, M.A. & Shahid, M.R. 2014. Complete degradation of dimethyl phthalate by biochemical cooperation of the Bacillus thuringiensis strain isolated from cotton field soil. RSC Advances 4: 55960-55966.

Wang, X.Y., Wu, X.B. & Xu, F.C. 2003. Purification and some properities of fibrinolytic enzyme from Pheretima. Pharmaceutical Biotechnology 10(2): 88-91.

Wei, H.X. 2010. Compare treatment of angina pectoris with lumbrokinase and aspirin. Capital Medicine 2: 47-48.

Wu, J.X. & Zhao, X.Y. 2004. Casein plate method for determination of earthworm fibrinolytic enzyme. Nature Magazine 26(3): 184-185.

Yang, J.S. & Ru, B.G. 1997. Purification and characterization of an sds-activated fibbrinolytic enzyme from Eisenia fetida. Comparative Biochemistry and Physiology 183(3): 623-631.

Yu, N.W., Liu, J., Wu, J.F., Xu, D.X., Yang, Y.S., Guo, F.Q., Xie, Y. & Sun, H. 2009. Clinical research of improve carotid atherosclerotic plaques in patients ischemic cerebrovascular disease with Lumbrokinase capsule. China Medicine 4(3): 169-170.

Zhou, H., Wang, C.W., Zhang, Y. & Liu, Y. 2011. Purification and characterization of lumbrokinas. Chinese Journal of Biochemical Pharmaceutics 32(3): 212-216.

Zhu, X.J., Tan, J.F., Zhu, Y., Ding, X., Jin, X.Y., He, H., Dong, P. & Yang, Y. 2009. Clinical studies of treatment of acute cerebral infarctionc with lumbrokinase capsule. Capital Medicine (16): 46-47.

*Corresponding author; email: zumingli2@gmail.com