Sains Malaysiana 45(4)(2016): 601–608

Treatment of Wastewater Originating from Aquaculture and Biomass Production in Laboratory Algae Bioreactor using Different Carbon Sources

(Rawatan Air Sisa daripada Pengeluaran Akuakultur dan Biojisim di Makmal Alga Bioreaktor Menggunakan Punca Karbon yang Berbeza)

 

KATYA N. VELICHKOVA^1*, IVAYLO N. SIRAKOV¹, GEORGI G. BEEV², STEFAN A. DENEV² & DIMITAR H. PAVLOV³

 

¹Department of Biology and Aquaculture, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

 

²Department of Biochemistry, Microbiology and Physics, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

 

³Department of Plant Production, Faculty of Agriculture, Trakia University, 6000 Stara Zagora

Bulgaria

 

Received: 31 May 2015/Accepted: 6 October 2015

 

ABSTRACT

The aim of present study was to explore the effect of different carbon sources on biomass accumulation in microalgae Nannochloropsis oculata and Tetraselmis chuii and their ability to remove N and P compounds during their cultivation in aquaculture wastewater. Microalgae cultivation was performed in laboratory bioreactor consisted from 500 mL Erlenmeyer flasks, containing 250 mL wastewater from semi closed recirculation aquaculture system. The cultures were maintained at room temperature (25-27ºC) on a fluorescent light with a light: dark photoperiod of 15 h: 9 h. The microalgae species were cultivated in wastewater with different carbon sources: glucose, lactose and saccharose. The growth of strains was checked for 96 h period. In the present study, N. oculata and T. chuii showed better growth in wastewater from aquaculture with saccharose carbon source during the experiment. The most effective reduce of nitrate and total nitrogen was proved in N. oculata cultivated in wastewater with glucose as carbon source. T. chuii cultivated in wastewater containing glucose showed 8.27% better cleaning effect in ammonium compared with N. oculata. T. chuii grew in wastewater with glucose as carbon source showed 19.5% better removal effect in phosphate compared with N. oculata strain.

 

Keywords: Biomass; Nannochloropsis oculata; Tetraselmis chuii; wastewater

 

ABSTRAK

Tujuan kajian ini dijalankan adalah untuk mengkaji kesan punca karbon yang berbeza terhadap pengumpulan biojisim pada mikroalga Nannochloropsis oculata dan Tetraselmis chuii serta keupayaan mereka untuk mengeluarkan sebatian N dan P semasa penanaman di dalam akuakultur air sisa. Penanaman mikroalga dijalankan dalam bioreaktor makmal yang terdiri daripada 500 termos mL Erlenmeyer, yang mengandungi 250 mL air sisa daripada sistem edaran semula akuakultur separuh tertutup. Kultur dikekalkan pada suhu bilik (25-27ºC dengan cahaya lampu neon: fotokala gelap 15 h: 9 h. Spesies mikroalga telah ditanam dalam air sisa dengan punca karbon berbeza: glukosa, laktosa dan sakarosa. Pertumbuhan strain telah dipantau untuk tempoh 96 jam. Dalam kajian ini N. oculata dan T. chuii menunjukkan pertumbuhan yang lebih baik dalam air sisa oleh akuakultur, dengan punca karbon sakarosa semasa eksperimen. Paling berkesan mengurangkan nitrat dan jumlah nitrogen telah dibuktikan dalam N. oculata yang ditanam di dalam air sisa dengan glukosa sebagai punca karbon. T. chuii yang ditanam dalam air sisa mengandungi glukosa menunjukkan kesan pembersihan 8.27% lebih baik dalam ammonium berbanding dengan N. oculata. T. chuii yang membesar dalam air sisa dengan menggunakan glukosa sebagai punca karbon menunjukkan kesan penyingkiran 19.5% lebih baik dalam fosfat berbanding dengan strain N. oculata.

 

Kata kunci: Air sisa; biojisim; Nannochloropsis oculata; Tetraselmis chuii

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*Corresponding author; email: genova@abv.bg