Sains Malaysiana 46(12)(2017): 2273–2280

http://dx.doi.org/10.17576/jsm-2017-4612-03

 

Ovarian Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea Fed with Two Diets

(Peringkat Kematangan Ovari bagi Ketam Lumpur Liar dan Kurungan Scylla olivacea Diberi Makan dengan Dua Diet)

 

AZMIE GHAZALI1, NOORDIYANA MAT NOORDIN2, AMBOK BOLONG ABOL-MUNAFI1,

MOHAMAD N. AZRA1 & MHD IKHWANUDDIN1

 

 

1Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

 

2School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu,

21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

 

Received: 25 July 2016/Accepted: 25 May 2017

 

ABSTRACT

This study was aimed to determine the ovarian maturation stages of wild and captive orange mud crab, Scylla olivacea fed with different diets via gonadosomatic status, oocyte diameter and histological examinations. Captive crabs were fed with blood cockle, Anadara granosa, or fish, Decapterus spp. Through the histological examinations, ovarian maturation stages of wild and captive S. olivaceawas classified into four stages: Immature (Stage 1), Early maturing (Stage 2), Pre-maturing (Stage 3) and Fully matured (Stage 4). Gonadosomatic Index of wild and captive crabs remained low during immature and 2 but increased significantly (p<0.05) in pre-maturing and 4 ovaries. Oocytes size were significantly different (p<0.05) in all ovarian maturation stages of wild and captive crabs. Follicle cells surround the oocyte of immature ovary and small yolk globules start to appear in early maturing ovary with large nucleus size. Oocyte size increased significantly (p<0.05) and yolk globule obviously appeared in pre-maturing ovary. Large and fused yolk globules appeared in the oocytes of fully matured ovary with nucleus was barely visible. The present study revealed that, ovarian maturation stages of S. olivacea are closely related to its morphological appearance and cellular development.

 

Keywords: Aquatic science; fisheries; invertebrate; nutrition; reproductive biology

 

ABSTRAK

Kajian ini bertujuan untuk menentukan tahap pematangan ovari bagi ketah lumpur liar dan kurangan jenisScylla olivacea yang diberi makan dua diet yang berlainan dengan melalui status gonadosomatik, diameter oosit dan pemeriksaan histologi. Ketah lumpur telah diberi makanan seperti kerang darah seperti Anadara granosa, atau ikan seperti Decapterus spp. Peringkat pematangan ovari bagiS. olivacea liar dan kurungan telah dibahagi kepada empat peringkat melalui pemeriksaan histologi: Tidak matang (Tahap 1), Matang awal (Tahap 2), Pra-matang (Tahap 3) dan Matang sepenuh (Tahap 4). Indeks Gonadosomatik ketam lumpur liar dan kurungan adalah rendah pada tahap tidak matang dan 2 ovari tetapi meningkat dengan ketara (p<0.05) bagi pra-matang dan 4 ovari. Saiz oosit adalah berbeza dengan ketara (p<0.05) bagi semua peringkat pematangan ovari bagi ketam lumpur liar dan kurangan. Sel-sel folikel mengelilingi oosit ovuri yang tidak matang dan kuning telur yang kecil mula berlaku pada tahap matang awal ovari dengan saiz nukleus besar. Saiz oosit meningkat secara ketara (p<0.05) dan globul telur kuning jelas kelihatan pada tahap pra-matang ovari.

 

Kata kunci: Biologi pembakan; invertebrat; nutrisi; perikanan; sains akuatik

REFERENCES

 

Alava, V.R., Quinitio, E.T., Pedro, J.B.D., Orosco, Z.G.A. & Wille, M. 2007. Reproductive performance, lipids and fatty acids of mud crab Scylla serrata(Forsskal) fed dietary lipid levels. Aquaculture Research 38: 1442-1451.

Azra, M.N. & Ikhwanuddin, M. 2016. A review of maturation diets for mud crab genus Scylla broodstock: Present research, problems and future perspective. Saudi Journal of Biological Sciences 23: 257-267.

Chen, Y.N., Tseng, D.Y., Ho, P.Y. & Kuo, C.M. 1999. Site of vitellogenin synthesis determined from a cDNA encoding a vitellogenin fragment in the freshwater giant prawn. Macrobrachium rosenbergii. Molecular Reproduction & Development 54: 215-222.

Hagedorn, H.H. & Kunkel, J.G. 1979. Vitellogenin and vitellin in insect. Annual Review of Entomology 24: 475-505.

Ikhwanuddin, M., Mohamed, S., Rahim, A.I.A., Azra, M.N., Jaaman, S.A., Bolong, A.M.A. & Noordin, N.M. 2015. Observation on the effect of natural diets on ovarian rematuration in blue swimming crab Portunus pelagicus(Linnaeus, 1758). Indian Journal of Fisheries 62: 124-127.

Ikhwanuddin, M., Azmie, G., Juariah, H.M., Zakaria, M.Z. & Ambak, M.A. 2011. Biological information and population features of mud crab, genus Scylla from mangrove areas of Sarawak, Malaysia. Fisheries Research 108: 299-306.

Iromo, H., Zairin, M. Jr., Agus, S.M. & Manalu, W. 2015. Effectivy of thyroxine hormone suplementation in the ovarian maturation of females mud crab (Scylla serrata). Pakistan Journal of Biotechnology 11: 79-86.

Islam, M.S., Kodama, K. & Kurokura, H. 2010a. Ovarian development of mud crab, Scylla olivacea in Pak Phanang mangrove swamps, Thailand. Marine Biology Research 6: 503-510.

Islam, M.S., Kodama, K. & Kurokura, H. 2010b. Ovarian development of mud crab, Scylla paramamosain in tropical mangrove swamps, Thailand. Journal of Scientific Research 2: 380-389.

Keenan, C.P., Davie, P.J.F. & Mann, D.L. 1998. A revision of the genus Scylla De Haan, 1833 (Crustacea: Decapoda: Brachyura: Portunidae). The Raffles Bulletin of Zoology 46: 217-245.

Kodama, K., Shimizu, T., Yamakawa, T. & Aoki, I. 2004. Reproductive biology of the female Japanese mantis shrimp Oratosquilla oratoria(Stomatopoda) in relation to changes in the seasonal pattern of larval occurrence in Tokyo Bay, Japan. Fisheries Science 70: 734-745.

Pattiasina, B.J., Jamal, E. & Pattinasarany, A.Y. 2012. The effect of different feed on the spawning performance of mud crab Scylla serratabroodstock. Jurnal Akuakultur Indonesia 11: 153-161.

Quinitio, E.T., Pedro, J.D. & Parado-Estepa, F.D. 2007. Ovarian maturation stages of mud crab Scylla serrata. Aquaculture Research 38: 1434-1441.

Ravid, T., Tietz, A., Khayat, M., Boehm, E., Michelis, R. & Lubzens, E. 1999. Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus(De Haan). Journal of Experimental Biology 202: 1819-1829.

Semenkova, B., Bayunova, L.V., Webb, M.A.H., Kolmakov, N.N., Romanov, A.G. & Barranikova, I.A. 2006. Effect of progentins on germinal vesicle breakdown in sturgeon follicle in vitro. Journal of Applied Ichthyology 22: 353-357.

Tantikitti, C., Kaonoona, R. & Pongmaneerat, J. 2015. Fatty acid profiles and carotenoids accumulation in hepatopancreas and ovary of wild female mud crab (Scylla paramamosain, Estampador, 1949). Songklanakarin Journal of Science 7 Technology 37: 609-616.

Viswanathan, C. & Raffi, S.M. 2015. The natural diet of the mud crab Scylla olivacea(Herbst, 1896) in Pichavaram mangroves, India. Saudi Journal of Biological Sciences 22: 698-705.

Yamada, R., Kodama, K., Yamakawa, T., Horiguchi, T. & Aoki, I. 2007. Growth and reproductive biology of the small penaeid shrimp Trachysalambria curvirostrisin Tokyo Bay. Marine Biology 151: 961-971.

Yano, I. & Chinzei, Y. 1987. Ovary is the site of vitellogenin synthesis in kuruma prawn, Penaeus japonicus. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 86: 213-218.

 

*Corresponding author; email: ikhwanuddin@umt.edu.my

 

 

 

previous