Sains Malaysiana 46(9)(2017): 1611–1616

http://dx.doi.org/10.17576/jsm-2017-4609-33

 

Kesan Ekstrak Akueus Rosel (Hibiscus sabdariffa Linn.) terhadap Sperma dan Testis Tikus Diadministrasi Nikotin

(Effects of Aqueous Extract of Roselle (Hibiscus sabdariffa Linn.) on Sperm and Testis in Rat-administered Nicotine)

 

IZATUS SHIMA TAIB, SITI BALKIS BUDIN*, MAIZATUL NADHIRAH ISMAIL,

SATIRAH ZAINALABIDIN & JAMALUDIN MOHAMED

 

Program Sains Bioperubatan, Pusat Pengajian Sains Diagnostik dan Kesihatan Gunaan

Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz

50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

Received: 14 July 2016/Accepted: 20 April 2017

 

ABSTRAK

Penghasilan radikal bebas oleh nikotin dikaitkan dengan kerosakan sistem pembiakan lelaki terutamanya sperma dan testis. Penggunaan rawatan yang berasaskan herba seperti Hibiscus sabdariffa Linn. (HSE) kian meningkat disebabkan kandungan antioksida semula jadi yang tinggi. Oleh itu, kajian ini dijalankan untuk mengkaji kesan ekstrak akueus HSE terhadap kualiti sperma dan tekanan oksidatif testis tikus yang diadministrasi nikotin. Sejumlah 21 ekor tikus jantan Sprague-Dawley dibahagikan secara rawak kepada tiga kumpulan iaitu kumpulan kawalan, nikotin dan nikotin+HSE. Nikotin disuntik secara intraperitoneum pada dos 0.6 mg/kg berat badan manakala HSE diberikan pada dos 100 mg/kg berat badan secara paksaan oral sebelum administrasi nikotin pada setiap hari selama 21 hari berturut-turut. Hasil kajian menunjukkan bilangan, motiliti dan viabiliti sperma lebih tinggi secara signifikan (p<0.05) manakala peratus ketaknormalan morfologi sperma lebih rendah secara signifikan (p<0.05) bagi pada kumpulan nikotin+HSE berbanding kumpulan nikotin. Sementara itu berlakunya penurunan aras malondialdehid (MDA) dan peningkatan aras glutation terturun (GSH) secara signifikan (p<0.05) bagi kumpulan nikotin+HSE berbanding kumpulan nikotin. Pemerhatian histologi mendapati HSE berpotensi melindungi morfologi testis tikus aruhan nikotin. Kesimpulannya, kajian ini menunjukkan bahawa pemberian suplemen ekstrak HSE berpotensi mencegah kerosakan sperma dan testis akibat administrasi nikotin.

 

Kata kunci: Antioksida; reproduktif; rokok; rosel; tekanan oksidatif

 

 

ABSTRACT

The generation of free radicals from nicotine is associated with reproductive damage especially on sperms and testis. The usage of treatment based on herbs such as Hibiscus sabdariffa Linn. (HSE) is gradually increased due to the high content of natural antioxidant. Therefore, this study was aimed to investigate the effects of extract aqueus HSE on sperms and testis in rat-administered nicotine. A total of 21 male Sprague Dawley rats were divided into three groups which were the control, nicotine and nicotine + HSE group. The nicotine was injected via intraperitoneum at the dose of 0.6 mg/kg body weight, while the HSE was given at the dose of 100 mg/kg body weight, orally before the nicotine administration for 21 censequtive days. The findings showed significantly (p<0.05) increased of sperm count, motility and viability while significantly (p< 0.05) decreased of percentage of abnormal sperm morphology in the nicotine + HSE group compared with the nicotine group. Furthermore, the level of malondialdehyde (MDA) reduced significantly (p<0.05) and the level of glutathione (GSH) increased significantly (p<0.05) in the nicotine + HSE group compared with the nicotine group. Histology study showed the potential of HSE in protecting the testis morphological alteration induced by nicotine. In conclusion, supplementation of HSE has the potential to prevent sperm and testis damage induced by nicotine.

 

Keywords: Antioxidant; oxidative stress; reproductive; roselle; cigarette

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*Corresponding author; email: balkis@ukm.edu.my