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Sains Malaysiana 51(7)(2022):
http://doi.org/10.17576/jsm-2022-5107-07
Identification of Pathogenic Fungi
Causing Leaf Spot of Urtica cannabina and Malus sieversii in the Wild Fruit
Forest of Tianshan Mountain, Xinjiang, China
(Pengenalpastian Kulat Patogen
Menyebabkan Bintik Daun Urtica cannabina dan Malus sieversii di Hutan Buah Liar Gunung Tianshan,
Xinjiang, China)
JIESHI
TANG1,2, YU LIU2, CHI ZHANG3, CAIXIA HAN1 & HUA SHAO1,4*
1State Key Laboratory of Desert
and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy
of Sciences, Urumqi, 830011, China
2College of Life Sciences,
Sichuan University, Chengdu, 610065, China
3Shandong Provincial Key
Laboratory of Water and Soil Conservation and Environmental Protection, College
of Resources and Environment, Linyi University, Linyi 276000, China
4Research Center for Ecology
and Environment of Central Asia, Xinjiang Institute of Ecology and Geography,
Chinese Academy of Sciences, Urumqi, 830011, China
Received: 28 November
2020/ Accepted: 12 January 2022
Abstract
Degradation of the wild apple trees in the wild fruit forest of
Tianshan mountain of Xinjiang Province, China, has attracted great attention in
recent years, and pathogens are believed to be an important responsible factor.
We observed that Malus sieversii and
its understory plant, Urtica cannabina,
exhibited similar symptoms of leaf spot disease, and we suspect that they are
caused by the same pathogens. DNA sequencing using ITS1 and ITS4 primers was applied
to identify the pathogenic fungi from diseased leaves of U. cannabina and M. sieversii, which led to the
identification of Alternaria sp. and Fusarium sp. as active pathogens causing same
symptoms on leaves of both species. Our results implied that these two plants
shared the same pathogenic fungi that cause leaf spot disease, and infection of
the understory species U. cannabina might provide a reservoir of the pathogens which can attack M. sieversii and contribute at least in
part, to the degradation of M. sieversii.
Keywords: Leaf
spot; Malus sieversii; pathogenic fungi; rDNA-ITS; Urtica cannabina
Abstrak
Kemerosotan pokok epal liar di hutan buah liar gunung Tianshan di
wilayah Xinjiang, China, telah menarik perhatian sejak beberapa tahun
kebelakangan ini dan patogen dipercayai menjadi faktor utama yang terlibat.
Kami memerhatikan bahawa Malus sieversii dan tumbuhan bawahnya, Urtica
cannabina, menunjukkan simptom penyakit tompok daun yang sama dan kami
mengesyaki bahawa ia disebabkan oleh patogen yang sama. Penjujukan DNA
menggunakan pencetus ITS1 dan ITS4 digunakan untuk mengenal pasti kulat patogen
daripada daun U. cannabina dan M. sieversii yang berpenyakit. Ini
membawa kepada pengenalpastian Alternaria sp. dan Fusarium sp.
sebagai patogen aktif yang menyebabkan gejala yang sama pada daun kedua-dua
spesies. Hasil menunjukkan bahawa kedua-dua tumbuhan ini berkongsi kulat
patogen yang sama yang menyebabkan penyakit bintik daun, dan jangkitan spesies U.
cannabina mungkin menyediakan takungan patogen yang boleh menyerang M.
sieversii dan sekurang-kurangnya menyumbang sebahagian kepada kemerosotan M.
sieversii.
Kata kunci: Kulat patogen; Malus sieversii; rDNA-ITS; tompok
daun; Urtica cannabina
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