Sains Malaysiana 44(11)(2015): 1593–1598


Influence of Benzyltriethylammonium Chloride on Biocorrosion Activity of Consortium Bacteria from Tropical Crude Oil

(Pengaruh Benziltrietilamonium Klorida terhadap Aktiviti Biokakisan Konsortium Bakteria daripada Minyak Mentah Tropika)



1School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor Darul Ehsan, Malaysia


2School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Pulau Pinang, Malaysia


3School of Environment and Natural Resources Science, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia


Diserahkan: 28 Mac 2015/Diterima: 3 Julai 2015



The performance of pipeline system used in petroleum industry is crucially declined by natural microbial activities and demanding extra operational cost. Requirement on high capability of functional substances is attracting worldwide research interest. The aim of this paper was to study the effectiveness of benzyltriethylammonium chloride (BTC) on reducing the activity of a consortium bacteria consisting of sulfate-reducing bacteria (C-SRB). C-SRB was isolated from tropical crude oil and enumeration of this consortium was measured by viable cell count technique. The effectiveness of BTC was calculated from potentiodynamic polarization method and biofilm analysis was performed by scanning electron microscope. The viable cell count technique indicated that the maximum growth of C-SRB was approximately 160 trillion CFU/mL at 7 days incubation period. BTC was capable of reducing biocorrosion activity due to adsorption process and mitigating SRB species. Biofilm analysis has proven that C-SRB activity is minimized due to less presence of bacterial growth, extracellular polymeric substances and corrosion product. In conclusion, BTC is capable to inhibit C-SRB activity on biocorrosion of carbon steel pipeline.


Keywords: Carbon steel; consortium bacteria; potentiodynamic polarization



Keupayaan sistem saluran paip yang digunakan dalam industri petroleum banyak mengalami penyusutan akibat aktiviti mikrob dan memerlukan kos pengoperasian yang tinggi. Keperluan keupayaan yang tinggi daripada sebatian berfungsi telah menarik minat penyelidikan seluruh dunia. Matlamat kajian ini adalah untuk mengkaji kecekapan benziltrietilamonium klorida (BTC) terhadap penurunan aktiviti konsortium bakteria yang mengandungi bakteria penurun sulfat (C-SRB). C-SRB telah dipencilkan daripada minyak mentah tropika dan pengangkaan konsortium ini telah dihitung melalui teknik kiraan sel boleh hidup. Kecekapan BTC ditentukan daripada kaedah kekutuban keupayaan dinamik dan analisis biofilem telah dijalankan dengan menggunakan mikroskop elektron imbasan. Teknik kiraan sel boleh hidup mendapati nilai pertumbuhan maksimum C-SRB dianggarkan berjumlah 160 trillion CFU/mL dalam tempoh 7 hari pengeraman. BTC didapati berupaya mengurangkan aktiviti biokakisan melalui proses penjerapan dan pengurangan spesies SRB. Analisis biofilem membuktikan bahawa aktiviti C-SRB adalah minimum dengan penggunaan BTC disebabkan kurangnya pertumbuhan bakteria, juga sebatian polimer ekstrasel dan hasil kakisan. Sebagai kesimpulan, BTC didapati berupaya menyekat aktiviti C-SRB dan proses biokakisan pada permukaan paip keluli karbon.


Kata kunci: Kekutuban keupayaan dinamik; keluli karbon; konsortium bakteria


Abdullah, A., Yahaya, N., Md Noor, N. & Mohd Rasol, R. 2014. Microbial corrosion of Api 5l X-70 carbon steel by Atcc 7757 and consortium of sulfate-reducing bacteria. Journal of Chemistry 2014(1): 1-7.

Ahamad, I., Prasad, R., Ebenso, E.E. & Quraishi, M.A. 2012. Electrochemical and quantum chemical study of Albendazole as corrosion inhibitor for mild steel in hydrochloric acid solution. International Journal of Electrochemical Science 7: 3436-3452.

Ahmad, Z. 2006. Principles of Corrosion Engineering and Corrosion Control. Oxford: Butterworth-Heinemann.

Aiad, I., El-Sukkary, M.M., Soliman, E.A., El-Awady, M.Y. & Shaban, S.M. 2014. Characterization, surface properties and biological activity of new prepared cationic surfactants. Journal of Industrial and Engineering Chemistry 20(4): 1633-1640.

Al-Amiery, A., Kadhum, A., Alobaidy, A.H., Mohamad, A. & Hoon, P. 2014. Novel corrosion inhibitor for mild steel in Hcl. Materials 7(2): 662-672.

Al-Jaroudi, S.S., Ul-Hamid, A. & Al-Gahtani, M.M. 2011. Failure of cruide oil pipeline due to microbiologically induced corrosion. Corrosion Engineering, Science and Technology 46(4): 568-579.

Al-Sabagh, A.M., Kandil, N.G., Ramadan, O., Amer, N.M., Mansour, R. & Khamis, E.A. 2011. Novel cationic surfactants from fatty acids and their corrosion inhibition efficiency for carbon steel pipelines in 1 M Hcl. Egyptian Journal of Petroleum 20(2): 47-57.

Alabbas, F.M., Spear, J.R., Kakpovbia, A., Balhareth, N.M., Olson, D.L. & Mishra, B. 2012. Bacterial attachment to metal substrate and its effects on microbiologically-influenced corrosion in transporting hydrocarbon pipeline. Journal of Pipeline Engineering 11(3): 63-72.

Badawi, A.M., Hegazy, M.A., El-Sawy, A.A., Ahmed, H.M. & Kamel, W.M. 2010. Novel quaternary ammonium hydroxide cationic surfactants as corrosion inhibitors for carbon steel and as biocides for sulfate reducing bacteria (Srb). Materials Chemistry and Physics 124(1): 458-465.

Dkhireche, N., Dahami, A., Rochdi, A., Hmimou, J., Touir, R., Ebn Touhami, M., El Bakri, M., El Hallaoui, A., Anouar, A. & Takenouti, H. 2013. Corrosion and scale inhibition of low carbon steel in cooling water system by 2-Propargyl- 5-O-Hydroxyphenyltetrazole. Journal of Industrial and Engineering Chemistry 19(6): 1996-2003.

Hegazy, M.A., Abdallah, M., Awad, M.K. & Rezk, M. 2014. Three novel di-quaternary ammonium salts as corrosion inhibitors for Api X65 steel pipeline in acidic solution. Part I: Experimental results. Corrosion Science 81: 54-64.

Idris, M.N., Daud, A.R. & Othman, N.K. 2015. Effectiveness of benzyl triethylammonium chloride for corrosion control on carbon steel. Journal of Applied Science and Agriculture 10(5): 52-57.

Idris, M.N., Daud, A.R., Othman, N.K. & Jalar, A. 2013. Corrosion control by benzyl triethylammonium chloride: Effects of temperture and its concentration. International Journal of Engineering & Technolgy 13(3): 47-51.

Kakooei, S., Mokhtar, C.I. & Ariwahjoedi, B. 2012. Mechanisms of microbiologically influenced corrosion: A review. World Applied Sciences Journal 17(4): 524-531.

Li, F., An, M., Liu, G. & Duan, D. 2009. Effects of sulfidation of passive film in the presence of Srb on the pitting corrosion behaviors of stainless steels. Materials Chemistry and Physics 113(2-3): 971-976.

Mahat, N.A., Othman, N.K., Sahrani, F.K. & Idris, M.N. 2015. Microbial corrosion of carbon steel by tropical environment consortium bacteria containing Srb. American-Eurasian Journal of Sustainable Agriculture 9(2): 29-34.

Mohamad, N., Jalar, A. & Othman, N.K. 2014. Surface morphology study on aluminum alloy after treated with silicate-based corrosion inhibitor from paddy residue. Sains Malaysiana 43(6): 935-940.

Moiseeva, L.S. & Kondrova, O.V. 2005. Biocorrosion of oil and gas field equipment and chemical methods for its suppression. I. Protection of Metals 41(4): 385-393.

Musa, A.Y., Kadhum, A.A.H., Mohamad, A.B., Takriff, M.S. & Chee, E.P. 2012. Inhibition of aluminum corrosion by phthalazinone and synergistic effect of halide ion in 1.0 M Hcl. Current Applied Physics 12(1): 325-330.

Negm, N.A., Kandile, N.G., Badr, E.A. & Mohammed, M.A. 2012. Gravimetric and electrochemical evaluation of environmentally friendly nonionic corrosion inhibitors for carbon steel in 1.0 M Hcl. Corrosion Science 65: 94- 103.

Ortega Morente, E., Fernández-Fuentes, M.A., Grande Burgos, M.J., Abriouel, H., Pérez Pulido, R. & Gálvez, A. 2013. Biocide tolerance in bacteria. International Journal of Food Microbiology 162(1): 13-25.

Sahrani, F.K., Ibrahim, Z., Yahya, A. & Aziz, M. 2008a. Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio Sp. and Citrobacter freundii from Pasir Gudang, Malaysia. Sains Malaysiana 37(4): 365-371.

Sahrani, F.K., Madzlan, A.A., Zaharah, I. & Adibah, Y. 2008b. Surface analysis of marine sulphate reducing bacteria exopolymers on steel during biocorrosion using X-Ray photoelectron spectroscopy. Sains Malaysiana 37(2): 131- 135.

Sheng, G-P., Yu, H-Q. & Li, X-Y. 2010. Extracellular polymeric substances (Eps) of microbial aggregates in biological wastewater treatment systems: A review. Biotechnology Advances 28(6): 882-894.

Yadav, M., Yadav, P.N. & Sharma, U. 2013. Substituted imidazoles as corrosion inhibitor for N80 steel in hydrochloric acid. Indian Journal of Chemical Technology 20: 363- 370.



*Pengarang untuk surat-menyurat; email: