Sains Malaysiana 44(12)(2015): 1739–1744

Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic Acid by Supercritical Carbon Dioxide Technology using Response Surface Methodology

(Pengoptimuman Kaedah Penyediaan Berterusan Arthrospira platensis γ Asid Linolenik oleh Teknologi Supergenting Karbon Dioksida yang menggunakan Kaedah Gerak Balas Permukaan)

YAO-CHING HSUEH^1,2, BE-JEN WANG¹, ZER-RAN YU³, CHENG-CHI WANG⁴ & MALCOLM KOO*⁵

¹Department of Food Science, National Chiayi University, Chiayi 60004, Taiwan, ROC

²Biotechnology Research Center, Far East University, Tainan 74448, Taiwan, ROC

³Superwell Biotechnology Corporation, Taichung 40876, Taiwan, ROC

⁴Graduate Institute of Precision Manufacturing, National Chin-Yi University of Technology, Taichung 41170, Taiwan, ROC

⁵Department of Medical Research, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi 62247, Taiwan, ROC

Diserahkan: 25 Ogos 2014/Diterima: 23 Jun 2015

ABSTRACT

γ-linolenic acid is an essential omega-6 unsaturated fatty acid made in the human body from linoleic acid. It can be metabolized to various important eicosanoids and it is also a precursor of prostaglandin E and several other active substances that are associated with anti-inflammatory properties. Arthrospira platensis is known to contain relatively large quantities of γ-linolenic acid. The aim of this study was to investigate the optimal parameters under a continuous preparation method of γ-linolenic acid from A. platensis using supercritical carbon dioxide technology. A Box-Behnken experimental design and response surface methodology were used to optimize combinations among pressure (10, 20 and 30 MPa), temperature (40, 50 and 60°C) and flow rate of A. platensis extract liquor (1, 2 and 3 mL/min) for yield of γ-linolenic acid. The results showed that the extraction of γ-linolenic acid from A. platensis was optimized at a temperature of 60°C, a pressure of 30 MPa and a flow rate of 3 mL/min. These parameters could be used as a basis for facilitating future scale-up industrial applications.

Keywords: Arthrospira platensis; Spirulina platensis; supercritical carbon dioxide fractionation; γ-linolenic acid

ABSTRAK

Asid linolenik γ adalah asid lemak tak tepu penting omega-6 yang dihasilkan dalam tubuh manusia daripada asid linolik. Ia boleh dimetabolismakan untuk pelbagai eicosanoid penting dan ia juga merupakan pelopor prostaglandin E dan beberapa bahan aktif lain yang berkaitan dengan sifat anti-radang. Arthrospira platensis diketahui mengandungi kuantiti asid Linolenik γ yang agak besar. Kajian ini bertujuan untuk mengkaji parameter optimum dengan kaedah penyediaan berterusan asid Linolenik γ daripada A. platensis menggunakan teknologi supergenting karbon dioksida. Satu reka bentuk eksperimen Box-Behnken dan kaedah gerak balas permukaan digunakan untuk mengoptimumkan gabungan antara tekanan (10, 20 dan 30 MPa), suhu (40, 50 dan 60°C) serta kadar aliran cecair ekstrak A. platensis (1, 2 dan 3 mL/min) untuk hasil asid Linolenik γ. Keputusan menunjukkan pengekstrakan asid linolenik γ daripada A. platensis dioptimumkan pada suhu 60°C, tekanan pada 30 MPa dan kadar aliran 3 mL/min. Parameter ini boleh digunakan sebagai asas bagi tujuan memberi kemudahan kepada aplikasi industri pada masa hadapan.

Kata kunci: Arthrospira platensis; Asid linolenik γ; pemeringkatan supergenting karbon dioksida; Spirulina platensis

RUJUKAN

Began, G., Goto, M., Kodama, A. & Hirose, T. 2000. Response surfaces of total oil yield of turmeric (Curcuma longa) in supercritical carbon dioxide. Food Research International 33(5): 341-345.

Box, G.E.P. & Benhnken, D.W. 1960. Some new three level design for the study of quantitative variable. Technometrics 2(4): 455-475.

Chamorro, G., Salazar, S., Favila-Castillo, L., Steele, C. & Salazar, M. 1997. Reproductive and peri- and postnatal evaluation of Spirulina maxima in mice. Journal of Applied Phycology 9(2): 107-112.

Cohen, Z., Reungjitchachawali, M., Siangdung, W. & Tanticharoen, M. 1993. Production and partial purification of γ-linolenic acid and some pigments from Spirulina platensis. Journal of Applied Phycology 5(1): 109-115.

Ishikawa, T., Fujiyama, Y., Igarashi, O., Morino, M., Tada, N., Kagami, A., Sakamoto, T., Nagano, M. & Nakamura, H. 1989. Effects of gammalinolenic acid on plasma lipoproteins and apolipoproteins. Atherosclerosis 75(2-3): 95-104.

Keen, H., Payan, J., Allawi, J., Walker, J., Jamal, G.A., Weir, A.I., Henderson, L.M., Bissessar, E.A., Watkins, P.J., Sampson, M., Gale, E.A.M., Scarpello, J., Boddie, H.G., Hardy, K.J., Thomas, P.K., Misra, P. & Halonen, J.P. 1993. Treatment of diabetic neuropathy with γ-linolenic acid. Diabetes Care 16(1): 8-15.

Marles, R.J., Barrett, M.L., Barnes, J., Chavez, M.L., Gardiner, P., Ko, R., Mahady, G.B., Low Dog, T., Sarma, N.D., Giancaspro, G.I., Sharaf, M. & Griffiths, J. 2011. United States pharmacopeia safety evaluation of spirulina. Critical Reviews in Food Science and Nutrition 51(7): 593-604.

Matsuno, T., Matsumoto, Y., Saito, M. & Morikawa, J. 1977. Isolation and characterization of cytotoxic diterpenoid isomers from propolis. Zeitschrift für Naturforschung C 52(9-10): 702-704.

Mendes, R.L., Reis, A.D. & Palavra, A.F. 2006. Supercritical CO2 extraction of γ-linolenic acid and other lipids from Arthrospira (Spirulina) maxima: Comparison with organic solvent extraction. Food Chemistry 99(1): 57-63.

Mendes, R.L., Reis, A.D., Pereira, A.P., Cardoso, M.T., Palavra, A.F. & Coelho, J.P. 2005. Supercritical CO2 extraction of γ-linolenic acid (GLA) from cyanobacterium Arthrospira (Spirulina) maxima: Experiments and modeling. Chemical Engineering Journal 105(3): 147-151.

Mendiola, J.A., Mart?nez, D.G., Ruperez, F.J., Mart?n-Alvarez, P.J., Reglero, G., Cifuentes, A., Barbas, C., Ibanez, E. & Senorans, F.J. 2008. Enrichment of vitamin E from Spirulina platensis microalga by SFE. Journal of Supercritical Fluids 43(3): 484-489.

Morse, P.F., Horrobin, D.F., Manku, M.S., Stewart, J.C., Allen, R., Littlewood, S.,

Puolakka, J., Makarainen, L., Viinikka, L. & Ylikorkala, O. 1985. Biochemical and clinical effects of treating the premenstrual syndrome with prostaglandin synthesis precursors. The Journal of Reproductive Medicine 30(3): 149-153.

Sajilata, M.G., Singhal, R.S. & Kamat, M.Y. 2008a. Supercritical CO2 extraction of γ-linolenic acid (GLA) from Spirulina platensis ARM 740 using response surface methodology. Journal of Food Engineering 84(2): 321-326.

Sajilata, M.G., Singhal, R.S. & Kamat, M.Y. 2008b. Fractionation of lipids and purification of γ-linolenic acid (GLA) from Spirulina platensis. Food Chemistry 109(3): 580-586.

Salazara, M., Mart?́neza, E., Madrigalb, E., Ruizc, L.E. & Chamorro, G.A. 1998. Subchronic toxicity study in mice fed Spirulina maxima. Journal of Ethnopharmacology 62(3): 235-241.

Tapiero, H., Ba, G.N., Couvreur, P. & Tew, K.D. 2002. Polyunsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies. Biomedicine and Pharmacotherapy 56(5): 215-222.

Vaddadi, K.S. 1992. Use of gamma-linolenic acid in the treatment of schizophrenia and tardive dyskinesia. Prostaglandins, Leukotrienes and Essential Fatty Acids 46(1): 67-70.

Ward, O.P. & Singh, A. 2005. Omega-3/6 fatty acids: Alternative sources of production. Process Biochemistry 40(12): 3627- 3652.

Yang, Y., Park, Y., Cassada, D.A., Snow, D.D., Rogers, D.G. & Lee, J. 2011. In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kützing and Spirulina plantensis. Food and Chemical Toxicology 49(7): 1560-1564.

Zurier, R.B., Rossetti, R.G., Jacobson, E.W., Demarco, D.M., Liu, N.Y., Temming, J.E., White, B.M. & Laposata, M. 1996. Gamma-linolenic acid treatment of rheumatoid arthritis: A randomized, placebo-controlled trial. Arthritis Rheumatism 39(11): 1808-1817.

*Pengarang untuk surat-menyurat; email: m.koo@utoronto.ca