Sains Malaysiana 44(4)(2015): 483–489

 

Growth and Anatomical Responses in Xanthostemon chrysanthus as Influenced by Paclobutrazol and Potassium Nitrate

(Tindak Balas Tumbesaran dan Anatomi Xanthostemon chrysanthus yang Dipengaruhi oleh Paklobutrazol

dan Kalium Nitrat)

 

 

M.R. AHMAD NAZARUDIN¹*, F.Y. TSAN², O. NORMANIZA³ & Y. ADZMI²

 

¹Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan

Malaysia

 

²Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA (UiTM)

40450 Shah Alam, Selangor Darul Ehsan, Malaysia

 

³Institute of Biological Sciences, Faculty of Science, Universiti Malaya (UM)

50603 Kuala Lumpur, Malaysia

 

Diserahkan: 26 September 2013/Diterima: 14 Disember 2014

 

ABSTRACT

A study was conducted to determine the effects of a plant growth regulator (paclobutrazol, PBZ) and commercial fertilizer (Krista-K Plus) as a source of potassium nitrate (KNO₃) on the growth of Xanthostemon chrysantus. It was also attempted to investigate the anatomical changes in the leaf and stem after the treatment. Nine treatments, i.e. control (no PBZ and Krista-K Plus application), 0 PBZ gL^-1 + 100 g Krista-K Plus, 0 PBZ gL^-1 + 200 g Krista-K Plus, 0.125 PBZ gL^-1 + 0 g Krista-K Plus, 0.125 PBZ gL^-1 + 100 g Krista-K Plus, 0.125 PBZ gL^-1 + 200 g Krista-K Plus, 0.25 PBZ gL^-1 + 0 g Krista-K Plus, 0.25 PBZ gL^-1 + 100 g Krista-K Plus and 0.25 PBZ gL^-1 + 200 g Krista-K Plus, were tested. PBZ was soil drenched at the commencement of the study while Krista-K Plus was applied at three-month intervals. Plant growth performances such as tree height, diameter at breast height, canopy diameter and leaf area were recorded monthly throughout the study period. Stem and leaf samples were collected before the application of treatments and after six months of treatments for anatomical observation by using electron microscope. Plant height, diameter at breast height, crown diameter and leaf area were significantly reduced with the application of PBZ. Palisade parenchyma thickness was increased by 33.83% with 0.25 PBZ gL^-1 + 200 g Krista-K Plus, while only 2.44% increment recorded in the control tree. Xylem thickness in the stem was reduced by 21.81% after treated with the highest dosage of PBZ, while the control tree only had 1.78% increment. Spongy parenchyma thickness in the leaf was unaffected. However, palisade parenchyma was found the thickest after combined treatment with 0.25 PBZ gL^-1 + 200 g Krista-K Plus. Micrograph images of the cross-section of leaf lamina and stem showed that the cells were tightly arranged in response to the application of PBZ.

 

Keywords: Growth inhibition; ornamental plant; plant anatomy; plant growth regulator; scanning electron microscope

 

ABSTRAK

Suatu kajian telah dijalankan untuk mengenal pasti kesan pengawal atur pertumbuhan pokok (paklobutrazol, PBZ) dan baja dagangan (Krista-K Plus) sebagai punca kalium nitrat (KNO₃) terhadap tumbesaran pokok Xanthostemon chrysanthus. Kajian ini juga bertujuan untuk mengkaji perubahan anatomi di dalam daun dan batang pokok selepas rawatan. Sembilan rawatan, iaitu kawalan (tanpa PBZ dan Krista-K Plus), 0 PBZ gL-1 + 100 g Krista-K Plus, 0 PBZ gL^-1 + 200 g Krista-K Plus, 0.125 PBZ gL^-1 + 0 g Krista-K Plus, 0.125 PBZ gL-1 + 100 g Krista-K Plus, 0.125 PBZ gL^-1 + 200 g Krista-K Plus, 0.25 PBZ gL^-1 + 0 g Krista-K Plus, 0.25 PBZ gL^-1 + 100 g Krista-K Plus dan 0.25 PBZ gL^-1 + 200 g Krista-K Plus, telah diuji. Aplikasi PBZ dilakukan secara siraman tanah pada permulaan kajian, manakala Krista-K Plus diberikan setiap tiga bulan. Tindak balas tumbesaran pokok seperti ketinggian pokok, garis pusat batang, garis pusat silara dan keluasan daun direkodkan setiap bulan sepanjang tempoh kajian. Sampel daun dan batang diambil sebelum rawatan dan selepas enam bulan rawatan untuk menilai perubahan anatominya menggunakan mikroskop elektron imbasan. Ketinggian pokok, garis pusat batang, garis pusat silara dan keluasan daun didapati berkurangan selepas rawatan PBZ. Ketebalan sel palisad parenkima dalam daun didapati meningkat sebanyak 33.83% selepas rawatan 0.25 PBZ gL^-1 + 200 g Krista-K Plus, manakala hanya 2.44% peningkatan direkodkan pada pokok kawalan. Ketebalan xilem di dalam batang berkurangan sebanyak 21.81% selepas dirawat dengan PBZ pada kepekatan yang paling tinggi, manakala pokok rawatan hanya meningkat sebanyak 1.78%. Ketebalan sel parenkima span di dalam daun pula tidak terjejas. Walau bagaimanapun, sel palisad parenkima didapati paling tebal selepas dirawat dengan 0.25 PBZ gL^-1 + 200 g Krista-K Plus. Imej mikrograf keratan rentas lamina daun dan batang pokok menunjukkan sel tersebut tersusun dengan padat akibat tindak balas terhadap rawatan PBZ.

 

Kata kunci: Anatomi pokok; mikroskop elektron imbasan; pengawal atur pertumbuhan pokok; perencatan pertumbuhan; pokok hiasan

RUJUKAN

Ahmad Nazarudin, M.R. 2012. Effects of paclobutrazol, uniconazole and flurprimidol on growth and flowering responses in potted Hibiscus rosa-sinensis L. Journal of Tropical Plant Physiology 4: 28-39.

Ahmad Nazarudin, M.R., Tsan, F.Y. & Mohd Fauzi, R. 2012. Morphological and physiological response of Syzygium myrtifolium (Roxb.) Walp. to paclobutrazol. Sains Malaysiana 41(10): 1187-1192.

Ahmad Nazarudin, M.R., Mohd Fauzi, R. & Tsan, F.Y. 2007. Effects of paclobutrazol on the growth and anatomy of stems and leaves of Syzygium campanulatum. Journal of Tropical Forest Science 19(2): 86-91.

Akhtar, M.E., Khan, M.Z., Ahmad, S., Ashraf, M. & Sardar, A. 2009. Effect of potassium on micromorphological and chemical composition of three cotton (Gossypium hirsutum L.) genotypes. African Journal of Biotechnology 8(15): 3511-3518.

Bai, S., Chaney, W. & Qi, Y. 2004. Response of cambial and shoot growth in trees treated with paclobutrazol. Journal of Arboriculture 30(3): 137-145.

El-Quesni, F.E.M., Kandil, M.M. & Mahgoub, M.H. 2007. Some studies on the effect of putrescine and paclobutrazol on the growth and chemical composition of Bougainvillea glabra L. at Nubaria. American-Eurasian Journal of Agricultural and Environmental Science 2(5): 552-558.

Fletcher, R.A., Gilley, A., Sankhla, N. & Davis, T.D. 2000. Triazoles as plant growth regulators and stress protectants. Horticultural Reviews 24: 55-138.

Francescangeli, N., Marinangeli, P. & Curvetto, N. 2007. Paclobutrazol for height control of two Lilium L.A. hybrids grown in pots. Spanish Journal of Agricultural Research 5(3): 425-430.

Gao, J., Hofstra, G. & Fletcher, R.A. 2011. Anatomical changes induced by triazoles in wheat seedlings. Canadian Journal of Botany 66(6): 1178-1185.

Gent, M.P. 2004. Efficacy and persistence of paclobutrazol applied to rooted cuttings before transplant. HortScience 39: 105-109.

Gopi, R., Jaleel, C.A. & Panneerselvam, R. 2008. Leaf anatomical responses of Amorphophallus campanulatus to triazoles fungicides. EurAsian Journal of BioSciences 2: 46-52.

Jaleel, C.A., Gopi, R., Azooz, M.M. & Panneerselvam, R. 2009. Leaf anatomical modifications in Catharanthus roseus as affected by plant growth promoters and retardants. Global Journal of Molecular Sciences 4(1): 1-5.

Jenks, M.A., Andersen, L., Teusink, R.S. & Williams, M.H. 2001. Leaf cuticular waxes of potted rose cultivars as affected by plant development, drought and paclobutrazol treatments. Plant Physiology 112: 62-70.

Karaguzel, O. & Ortacesme, V. 2002. Influence of paclobutrazol on the growth and flowering of Bougainvillea glabra 'Sanderiana'. Ziraat Fakultesi Dergisi, Akdeniz Universitesi 15(1): 79-84.

Karaguzel, O., Baktir, I., Cakmakci, S. & Ortacesme, V. 2004. Growth and flowering responses of Lupinus varius L. to paclobutrazol. HortScience 39(7): 1659-1663.

Kishorekumar, A., Jaleel, C.A., Manivannan, P., Sankar, B., Sridharan, R., Somasundaram, R. & Panneerselvam, R. 2006. Differential effects of hexaconazole and paclobutrazol on the foliage characteristics of Chinese potato (Solenostemon rotundifolius Poir., J.K. Morton). Acta Biologica Szegediensis 50(3-4): 127-129.

Kumar, S., Ghatty, S., Satyanarayana, J., Guha, A., Chaitanya, B.S.K. & Reddy, A.R. 2012. Paclobutrazol treatment as a potential strategy for higher seed and oil yield in field-grown Camelina sativa L. Crantz. BMC Research Notes 5: 137.

Mansuroglu, S., Karaguzel, O., Ortacesme, V. & Sayan, M.S. 2009. Effect of paclobutrazol on flowering, leaf and flower colour of Consolida orientalis. Pakistan Journal of Botany 41(5): 2323-2332.

Matsoukis, A.S., Sereli-Chronopoulou, A., Dimopoulus, I.D. & Kamoutsis, A. 2001. Responses of Lantana camara subsp. camara to paclobutrazol and shading. Canadian Journal of Plant Science 81(4): 761-764.

Mengel, S. 2007. Potassium. In Handbook of Plant Nutrition, edited by Barker, A.V. & Pilbean, D.J. New York: CRC Taylor and Francis. pp. 395-402.

Nie, L., Liu, H.X. & Chen, L.G. 2001. Effects of uniconazole on growth, photosynthesis and yield of Longan. ISHS Acta Hort. 558: I International Symposium on Litchi and Longan. http://www.actahort.org/books/558/558-46.htm. Retrieved March 13, 2004.

Pinto, A.C.R., Rodrigues, T.J.D., Leite, I.C. & Barbosa, J.C. 2005. Growth retardants on development and ornamental quality of potted 'lilliput' Zinnia elegans Jacq. Scientia Agricola 62(4): 337-345.

Rademacher, E. 2000. Growth retardants: Effects on gibberellin biosynthesis and other metabolic pathway. Annual Review of Plant Physiology and Plant Molecular Biology 51: 501-531.

Sebastian, B., Alberto, G., Emilio, A.C., Jose, A.F. & Juan, A.F. 2002. Growth, development and color response of potted Dianthus caryophyllus cv. Mondrian to paclobutrazol treatment. Scientia Horticulturae 1767: 1-7.

Sosef, M.S.M., Hong, L.T. & Prawirohatmodjo, S. 1998. Plant Resources of South-East Asia No. 5(3). Timber Trees: Lesser-known Timbers. Leiden: Back-huys Publishers.

Taiz, L. & Zeiger, E. 2006. Plant Physiology. 4th ed. Sunderland: Sinauer Associates, Inc., Publishers.

Tekalign, T., Hammes, P.S. & Robbertse, J. 2005. Paclobutrazol-induced leaf, stem, and root anatomical modifications in potato. HortScience 40(5): 1343-1346.

Tripathi, K.M. & Dhakal, D.D. 2005. Effect of paclobutrazol on off-season flower induction in acid lime (Citrus aurantifolia Swingle) landraces under Chitwan condition. Journal of Institute of Agriculture and Animal Science 26: 87-92.

Williams, D.R., Potts, B.M. & Smethurst, P.J. 2003. Promotion of flowering in Eucalyptus nitens by paclobutrazol was enhanced by nitrogen fertilizer. Canadian Journal of Forest Research 13: 74-81.

Yeshitela, T., Robbertse, P.J. & Stassen, P.J.C. 2004. Paclobutrazol suppressed vegetative growth and improved yield as well as fruit quality of 'Tommy Atkins' mango (Mangifera indica) in Ethiopia. New Zealand Journal of Crop and Horticultural Science 32: 281-293.

Zakaria, W. & Razak, A.R. 1999. SEM study of the morphology of leaves of four desert banana cultivars in Malaysia. Journal of Tropical Agriculture and Food Science 27: 151-158.

 

 

*Pengarang untuk surat-menyurat; email: nazarudin@frim.gov.my