Sains Malaysiana 47(11)(2018): 2647–2655

http://dx.doi.org/10.17576/jsm-2018-4711-07

 

The Effects of Temperature on Callus Induction and Regeneration in Selected Malaysian Rice Cultivar Indica

(Kesan Suhu ke atas Induksi Kalus dan Pertumbuhan Semula dalam Kultivar Indica Padi Malaysia Terpilih)

SURAIYA BINTE MOSTAFIZ, ALINA WAGIRAN*, NUR SYAFIQOH JOHAN, NUR SALIHA ABDLLAH ZULKIFLI & NG JA MING

 

Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Darul Takzim, Malaysia

 

Diserahkan: 3 Januari 2018/Diterima: 8 Julai 2018

 

ABSTRACT

The development of an efficient tissue culture protocol for somatic embryo would facilitate the genetic modification in breeding program. The present study describes the reproducible protocols for three wetland Malaysian rice cultivars (MR232, MR220 and MR220-CL2) and upland rice (Bario) via somatic embryogenesis. In the present study, four pre-heat treatments (35, 40, 45 and 50°C) were applied to mature seeds with different imbibition periods (3, 5 and 7 days) prior to culture on MS media with 3 mg/L 2,4-D. The results showed that the cultivars exhibited the highest callus induction percentage from 45°C pre-heated seeds and 3 days imbibition (100%, 96%, 100% and 95% for MR232, MR220, MR220-CL2 and Bario, respectively). Callus was induced early ranging from 3 to 12 days compared to without pre-heat treatment. The regeneration efficiency for MR220 and MR220-CL2 cultivars was significantly higher compared to the control treatment. However, both 45°C and 25°C (control) treatments produced higher plantlet regeneration for MR232 and Bario. This study observed that pre-heat treated seeds prior to callus induction did promote callusing and hence regeneration. These findings can be used to establish a suitable protocol for the in vitro regeneration system for several genetic improvements in the numerous stress tolerances of Malaysian rice.

 

Keywords: Bario; callus; MR220-Cl2; pre-heat treatment; regeneration; rice

 

ABSTRAK

Pembangunan protokol kultur tisu untuk penghasilan embrio somatik dapat membantu dalam pengubahsuaian genetik bagi rancangan pembiakbakaan. Kajian ini melaporkan protokol boleh ulang bagi tiga kultivar padi bendang Malaysia (MR232, MR220 dan MR220-CL2) dan padi bukit (Bario) melalui embriogenesis somatik. Dalam kajian ini, biji benih matang didedahkan kepada empat rawatan pra-pemanasan (35, 40, 45 dan 50°C) dengan masa pedapan yang berbeza (3, 5 dan 7 hari) sebelum dikultur pada medium MS yang mengandungi 3 mg/L 2,4-D. Keputusan kajian ini menunjukkan semua kultivar menghasilkan peratusan induksi kalus tertinggi daripada rawatan pra-pemanasan benih pada suhu 45°C dan 3 hari pedapan (100%, 96%, 100% dan 95% untuk MR232, MR220, MR220-CL2 dan Bario). Kalus diinduksi lebih awal pada kadar 3 ke 12 hari dibandingkan dengan tanpa rawatan pra-pemanasan. Keberkesanan penjanaan semula adalah lebih tinggi untuk kultivar MR220 dan MR220-CL2 dibandingkan dengan kawalan manakala kedua-dua rawatan pra-pemanasan 45°C dan kawalan mempunyai penjanaan semula anak pokok yang tinggi untuk MR232 dan Bario. Kajian ini menunjukkan bahawa rawatan pra-pemanasan ke atas biji benih sebelum penginduksian kalus menggalakkan pertumbuhan kalus dan juga penjanaan semula. Penemuan ini boleh digunakan untuk menghasilkan protokol yang sesuai untuk sistem penjanaan semula in vitro sebagai penambahbaikan kepada pelbagai jenis daya tahan padi Malaysia.

 

Kata kunci: Bario; kalus; MR220-CL2; padi; penjanaan semula; rawatan pra-pemanasan

RUJUKAN

Abdollahi, M.R., Darbandi, M., Hamidvand, Y. & Majdi, M. 2015. The influence of phytohormones, wheat ovary co-culture, and temperature stress on anther culture response of watermelon (Citrullus lanatus L.). Brazilian Journal of Botany 38(3): 447-456.

Abiri, R., Maziah, M., Shaharuddin, N.A., Yusof, Z.N.B., Atabaki, N., Hanafi, M.M., Sahebi, M., Azizi, P., Kalhori, N. & Valdiani, A. 2017. Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol. International Journal of Environmental Science and Technology 14(5): 1091-1108.

Afele, J.C., Kannenberg, L.W., Keats, R., Sohota, S. & Swanson, E.B. 1992. Increased induction of microspore embryos following manipulation of donor plant environment and culture temperature in corn (Zea mays L.). Plant Cell, Tissue and Organ Culture 28(1): 87-90.

Azizi, P., Rafii, M.Y., Mahmood, M., Abdullah, S.N.A., Hanafi, M.M., Latif, M.A., Sahebi, M. & Ashkani, S. 2017. Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae. Biotechnology & Biotechnological Equipment 31(1): 75-84.

Binte Mostafiz, S. & Wagiran, A. 2018. Efficient callus induction and regeneration in selected indica rice. Agronomy 8(5): 77.

Biswas, A. & Mandal, A. 2007. Plant regeneration in different genotypes of indica rice. Indian Journal of Biotechnology 6: 532–540.

Chaar, M., Pinker, I., Grieger, P. & Boehme, M. 2012. The effect of flower bud size and pre-treatment on callus induction and plant regeneration in anther culture of petunia “purple wave.” In VII International Symposium on in vitro Culture and Horticultural Breeding, edited by Geelen, D. Leuven: Int. Soc. Horticultural Science: 1: 375-382.

Chawla, H.S. 2002. Introduction to Plant Biotechnology. Enfield, NH, USA: Science Publishers, Inc.

Dewi, I., Purwoko, B., Aswidinnoor, H., Somantri, I. & Chozin, M. 2009. Plant regeneration from anther cultures of several genotypes of indica rice tolerant to aluminium toxicity. Indian Journal of Agricultural Science 2: 1-5.

Fageria, N.K. & Baligar, V.C. 2003. Upland rice and allelopathy. Communications in Soil Science and Plant Analysis 34(9- 10): 1311-1329.

FAOSTAT. Food crop production. 2015. Food and agriculture organization (FAO) Statistics Division. http://faostat.fao. org/site/567/default.aspx#ancor. Accessed on 5 May 2015.

Haq, I.U., Chang-Xing, Z., Mukhtar, Z., Jaleel, C.A. & Azooz, M.M. 2009. Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati. Journal of Plant Physiology 166(14): 1568-1575.

Haque, M., Islam, S.M.S. & Subramaniam, S. 2017. Effects of salt and heat pre-treatment factors on efficient regeneration in barley (Hordeum vulgare L.). 3 Biotech 7(1): 63.

Hirano, M., Sato, N. & Abe, K. 1999. Characterization of Ribes rubrum callus cultured under low temperature. Journal of Bioscience and Bioengineering 88(4): 416-420.

Htwe, N.N., Maziah, M., Ling, H.C., Zaman, F.Q. & Zain, A.M. 2011. Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress. African Journal of Biotechnology 10(3): 350-362.

Kadhimi, A.A., Zain, C.R.C.M., Alhasnawi, A.N., Isahak, A., Ashraf, M.F., Mohamad, A., Doni, F., Yusoff, W.M.W., Ibrahim, K., Badri, K.H., Jumali, M.H.H., Noorani, M.S.M., Ibrahim, N., Rasol, N.H.A. & Yaacob, W.Z.W. 2016. Impact of gamma rays exposure and growth regulators on Oryza sativa L. c.v MR269 callus induction. AIP Conference Proceedings 1784(1): 020006.

Kiviharju, E. & Pehu, E. 1998. The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis. Plant Cell, Tissue and Organ Culture 54(2): 97-104.

Kumar, A., Sood, A., Palni, L.M.S. & Gupta, A.K. 2015. In vitro propagation of Gladiolus hybridus hort.: Synergistic effect of heat shock and sucrose on morphogenesis. Plant Cell, Tissue and Organ Culture 57(2): 105-112.

Li, H., Qureshi, J.A. & Kartha, K.K. 1988. The influence of different temperature treatments on anther culture response of spring wheat (Triticum aestivum L.). Plant Science 57(1): 55-61.

Libin, A., King, P.J.H., Ong, K.H., Chubo, J.K. & Sipen, P. 2012. Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris. African Journal of Agricultural Research 7(30): 4260-4265.

Mohd Din, A.R.J., Iliyas Ahmad, F., Wagiran, A., Abd Samad, A., Rahmat, Z. & Sarmidi, M.R. 2016. Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi Journal of Biological Sciences 23(1): 69-77.

Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.

Naik, N., Rout, P., Umakanta, N., Verma, R.L., Katara, J.L., Sahoo, K.K., Singh, O.N. & Samantaray, S. 2017. Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell, Tissue and Organ Culture 128(3): 679-689.

Nicholas, D., Hazila, K.K., Chua, H.P. & Rosniyana, A. 2014. Nutritional value and glycemic index of Bario rice varieties. Journal of Tropical Agriculture and Food Science 42(1): 1-8.

Rahman, Z.A., Roowi, S., Wan Zaliha, W.S. & Subramaniam, S. 2010. Regeneration of Malaysian indica rice (Oryza sativa) variety MR232 via optimised somatic embryogenesis system. Journal of Phytology 2(3): 30-38.

Reddy, V.S., Leelavathi, S. & Sen, S.K. 1985. Influence of genotype and culture medium on microspore callus induction and green plant regeneration in anthers of Oryza sativa. Physiologia Plantarum 63(3): 309-314.

Silva, T.D. 2010. Indica rice anther culture: Can the impasse be surpassed? Plant Cell Tissue and Organ Culture 100(1): 1-11.

Touraev, A., Indrianto, A., Wratschko, I., Vicente, O. & Heberle- Bors, E. 1996. Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature. Sexual Plant Reproduction 9(4): 209-215.

Visarada, K.B.R.S., Sailaja, M. & Sarma, N.P. 2002. Effect of callus induction media on morphology of embryogenic calli in rice genotypes. Biologia Plantarum 45(4): 495-502.

Wang, X., Zhou, S., Chen, L., Quatrano, R.S. & He, Y. 2014. Phospho-proteomic analysis of developmental reprogramming in the moss Physcomitrella patens. Journal of Proteomics 108: 284-294.

Wong, S.C., Yiu, P.H., Bong, S.T.W., Lee, H.H., Neoh, P.N.P. & Rajan, A. 2009. Analysis of Sarawak Bario rice diversity using microsatellite markers. American Journal of Agricultural and Biological Sciences 4(4): 298-304.

Zuraida, A.R., Naziah, B., Zamri, Z. & Sreeramanan, S. 2011. Efficient plant regeneration of Malaysian indica rice MR 219 and 232 via somatic embryogenesis system. Acta Physiologiae Plantarum 33(5): 1913-1921.

 

*Pengarang untuk surat-menyurat; email: alina@utm.my

 

 

 

 

 

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