Sains Malaysiana 47(5)(2018): 1045–1050

http://dx.doi.org/10.17576/jsm-2018-4705-22

 

Assessing Diet of the Rufous-Winged Philentoma (Philentoma pyrhoptera) in Lowland Tropical Forest using Next-Generation Sequencing

(Penilaian Diet Filentoma Sayap Merah (Philentoma pyrhoptera) di Hutan Tropika Tanah Rendah menggunakan Penjujukan Generasi Seterusnya)

 

MOHAMMAD SAIFUL MANSOR^1*, SHUKOR MD. NOR¹ & ROSLI RAMLI²

 

¹School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

²Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

 

Diserahkan: 15 September 2017/Diterima: 19 Disember 2017

 

ABSTRACT

Dietary study provides understanding in predator-prey relationships, yet diet of tropical forest birds is poorly understood. In this study, a non-invasive method, next-generation sequencing (Illumina MiSeq platform) was used to identify prey in the faecal samples of the Rufous-winged Philentoma (Philentoma pyrhoptera). Dietary samples were collected in lowland tropical forest of central Peninsular Malaysia. A general invertebrate primer pair was used for the first time to assess diet of tropical birds. The USEARCH was used to cluster the COI mtDNA sequences into Operational Taxonomic Unit (OTU). OTU sequences were aligned and queried through the GenBank or Biodiversity of Life Database (BOLD). We identified 26 distinct arthropod taxa from 31 OTUs. Of all OTUs, there was three that could be identified up to species level, 20 to genus level, three to family level and five could not assigned to any taxa (the BLAST hits were poor). All sequences were identified to class Insecta belonging to 18 families from four orders, where Lepidoptera representing major insect order consumed by study bird species. This non-invasive molecular approach provides a practical and rapid technique to understand of how energy flows across ecosystems. This technique could be very useful to screen for possible particular pest insects consumed by insectivores (e.g. birds and bats) in crop plantation. A comprehensive arthropod studies and local reference sequen

 

Keywords: Dietary ecology; MiSeq; next-generation sequencing (NGS); Philentoma pyrhoptera; tropical birds

ABSTRAK

Kajian diet memberi pemahaman tentang hubungan antara pemangsa-mangsa, namun diet burung di hutan tropika kurang difahami. Dalam kajian ini, satu kaedah yang tidak invasif, penjujukan generasi akan datang (platform Illumina MiSeq) digunakan untuk mengenal pasti mangsa dalam sampel najis Filentoma Sayap Merah (Philentoma pyrhoptera). Sampel makanan diambil di hutan tropika tanah pamah di Semenanjung Malaysia. Set primer umum untuk invertebrata digunakan pertama kalinya untuk menilai diet burung tropika. Pautan USEARCH digunakan untuk mengkelompok jujukan mtDNA COI kepada Unit Operasi Taksonomi (OTU). Jujukan OTU telah disunting menggunakan perisian BioEdit dan ditentukan menerusi Pangkalan Data GenBank atau Biodiversity of Life Database (BOLD). Kami mengenal pasti 26 taksonomi arthropoda yang unik daripada 31 OTUs. Daripada semua OTUs, terdapat tiga yang boleh dikenal pasti hingga ke tahap spesies, 20 hingga genus, tiga hingga famili dan lima tidak dapat ditaksirkan (kadar BLAST yang rendah). Semua jujukan dikenal pasti sebagai kelas Insecta yang terdiri daripada 18 famili daripada empat order dengan Lepidoptera mewakili order serangga yang utama dimakan oleh spesies burung kajian. Pendekatan molekul yang tidak invasif ini menyediakan teknik yang praktikal dan cepat untuk memahami bagaimana tenaga mengalir merentasi ekosistem. Teknik ini juga sangat berguna untuk melihat kemungkinan serangga perosak yang tertentu dimakan oleh insektivor (contohnya, burung dan kelawar) di ladang tanaman. Kajian artropoda yang komprehensif dan jujukan rujukan tempatan perlu ditambah ke pangkalan data untuk meningkatkan peratusan jujukan yang boleh dikenal pasti.

 

Kata kunci: Burung tropika; ekologi pemakanan; MiSeq; penjujukan generasi akan datang (NGS); Philentoma pyrhoptera

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*Pengarang untuk surat-menyurat; email: msaifulmansor@ukm.edu.my