Sains Malaysiana 49(11)(2020): 2699-2714

http://dx.doi.org/10.17576/jsm-2020-4911-09                                                                  

 

Enantiomeric Separation of Azole Antifungal Compounds using Chromatographic and Electrophoretic Techniques: A Mini Review

(Pemisahan Enantiomerik Sebatian Antikulat Azol menggunakan Teknik Kromatografi dan Elektroforetik: Suatu Ulasan Mini)

 

NURUL RAIHANA BINTI AZHARI1, BOON YIH HUI1, NUR NADHIRAH MOHAMAD ZAIN1, FAIZ BUKHARI MOHD SUAH2, SHARIFAH MOHAMAD3,4, NOORFATIMAH YAHAYA1* & MUGGUNDHA RAOOV3,4*

 

1Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Penang, Malaysia

 

2School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia

 

3Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

 

4University of Malaya Centre for Ionic Liquids, Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

 

Diserahkan: 19 Mac 2020/Diterima: 12 Mei 2020

 

Abstract

The separation of azole antifungal chiral compounds has gaining much interests since 1980’s as they possess different biological, pharmaceutical, and pharmacokinetic behaviors at various toxicity levels. Numerous studies have looked into this subject matter, and therefore, this review presents an overview on different chiral separation techniques that have been developed such as liquid chromatography (LC), gas chromatography (GC), supercritical fluid chromatography (SFC), and capillary electrophoresis (CE). This comprehensive review also discusses the mechanisms of the developed analytical methods such as preparation techniques and optimization parameters towards the enantioseparation performances as well as their advantages and drawbacks. Lastly, the conclusion and future directions of the chiral separation, particularly for azole compounds are provided accordingly.

 

Keywords: Antifungal compounds; azole; capillary electrophoresis; chiral separation; chromatographic analysis

 

Abstrak

Pemisahan sebatian kiral antikulat azol telah menarik perhatian sejak tahun 1980 kerana ia mempunyai sifat biologi, farmaseutik dan farmakokinetik pada tahap ketoksikan yang berbeza. Oleh itu, kajian ini membentangkan gambaran keseluruhan mengenai teknik pemisahan kiral yang berbeza seperti kromatografi cecair (LC), kromatografi gas (GC), kromatografi cecair supergentingan (SFC) dan elektroforesis rerambut (CE). Kajian komprehensif ini juga membincangkan mekanisme bagi kaedah analisis yang maju seperti teknik penyediaan dan parameter pengoptimuman ke arah persembahan pemisahan enantio serta kelebihan dan kekurangannya. Akhir sekali, kesimpulan dan hala tuju masa depan dalam pemisahan kiral, terutamanya bagi sebatian azol diberikan dengan sewajarnya.

 

Kata kunci: Analisis kromatografik; azol; pemisahan kiral; rerambut elektroforesis; sebatian antikulat

 

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