Sains Malaysiana 49(11)(2020): 2735-2744

http://dx.doi.org/10.17576/jsm-2020-4911-12

 

A Tyrosine-Based Nanosensor for Rapid Sensitive Detection of Copper (II) Ions

(Pengesan Nano Berasaskan Tirosina untuk Pengesanan Sensitif Pantas Ion Tembaga (II))

 

JIAQI LIAN1,2, PANDENG MIAO2,3, NA LI2,3, ABDUL JAMIL KHAN2, XIANG JI1* & FENG ZHANG1,2,4

 

1School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, P. R. China

 

2Biomedical Nanocenter, School of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, P. R. China

 

3Terahertz Technology Innovation Research Institute, Shanghai Key Laboratory of Modern Optical System, Terahertz Science Cooperative Innovation Center, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, P. R. China

 

4Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital, Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, P. R. China

 

Diserahkan: 17 Januari 2020/Diterima: 8 Jun 2020

 

ABSTRACT

Most of the chromophores of fluorescent peptides contain aromatic amino acids with conjugated double bonds, among which tyrosine (Y) has become the focus of researches due to its unique physicochemical (optical, redox, and metal chelation) properties. However, there are few studies on the self-assembly and polymerisation of single. This study shows that the phenol group of Y can be oxidized into benzoquinone group in alkaline conditions and then undergoes polymerisation and further self-assembles into nanoparticles (NPs). The product of pYoxNPs have a strong fluorescence emission peak at 463 nm, and Cu2+ can spontaneously bind to it and dramatically quench their fluorescence. Based on these findings, we developed a rapid, sensitive and specific nanosensor for detecting Cu2+. When the concentration of Cu2+ is within the range of 40 μM - 1 mM, we can obtain a good linear correlation between the fluorescence intensity of pYoxNPs and the concentration of copper ions, and the limit of detection (LOD) is determined to 37.26 μM. In comparison to other modern methods for sensing Cu2+, this method has advantages of simplicity of material synthesis, low cost, robust and rapid in sensing reaction, so we envision a good prospect for Cu2+ detection applications in both bulk and harsh environments.

 

Keywords: Copper ion; fluorescence quenching; oxidation; polymerisation; tyrosine

 

ABSTRAK

Kebanyakan kromofor peptida pendarfluor mengandungi asid amino aromatik dengan ikatan berganda konjugasi, antaranya tyrosin (Y) telah menjadi tumpuan penyelidikan kerana ciri unik fizikokimianya (optik, redoks dan pengkelatan logam). Walau bagaimanapun, terdapat beberapa kajian mengenai pemasangan diri dan pempolimeran tunggal Y. Dalam kajian ini, kumpulan fenol Y boleh dioksidakan kepada kumpulan benzoquinon dalam keadaan alkali dan kemudian menjalani pempolimeran dan seterusnya menyambung diri ke nanozarah (NPs). pYoxNPs produk mempunyai puncak pelepasan pendarfluor yang kuat pada 463 nm dan Cu2+ secara spontan dapat mengikat mereka dan secara mendadak memadamkan pendarfluor mereka. Berdasarkan penemuan ini, kami membangunkan pengesan nano pesat, sensitif dan khusus untuk mengesan Cu2+. Apabila kepekatan Cu2+ berada dalam julat 40 μM - 1 mM, kita boleh mendapatkan korelasi linear yang baik antara keamatan pendarfluorpYoxNPs dan kepekatan ion tembaga, dan had pengesanan (LOD) ditentukan kepada 37.26 μM. Berbanding dengan kaedah moden yang lain untuk mengesan Cu2+, ini memperlihatkan kelebihan dalam kesederhanaan sintesis bahan, kos rendah dan mudah diperoleh, cepat dan pantas dalam reaksi penderiaan, jadi kami membayangkan prospek yang baik untuk aplikasi pengesanan Cu2+ dalam persekitaran pukal dan mencabar.

 

Kata kunci: Ion tembaga; pelindapkejutan pendarfluor; pengoksidaan; pempolimeran; tirosina

 

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*Pengarang untuk surat-menyurat; email: jixiang@imust.cn

   

 

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