Sains Malaysiana 49(11)(2020): 2793-2800

http://dx.doi.org/10.17576/jsm-2020-4911-18

 

Green Tea (Camellia sinensis) Extract Reduces Peptic Ulcer Induced by Helicobacter pylori in Sprague Dawley Rats

(Ekstrak Teh Hijau (Camellia sinensis) Mengurangkan Ulser Peptik yang Diaruh oleh Helicobacter pylori pada Tikus Sprague Dawley)


MARHANIS SALIHAH OMAR2, NUR NADIANA ADNAN1, ENDANG KUMOLOSASI1*, NORAZRINA AZMI1, NOOR SAFWAH DAMANHURI1 & FHATAHEYA BUANG1

 

1Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

2The Center of Quality Management of Medicine, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

Diserahkan: 26 November 2019/Diterima: 16 Mei 2020

 

ABSTRACT

The study was conducted to evaluate the anti-peptic ulcer activity of ethanol extract of Camellia sinensis (green tea) induced by Helicobacter pylori in Sprague Dawley rats. The standard regimen of anti-peptic ulcer agents, clarithromycin 30 mg/kg, amoxicillin 30 mg/kg and esomeprazole 3 mg/kg used as the positive control. The rats divided into 6 groups (n = 6), normal control, vehicle control (2% of tragacanth suspension), positive control for preventive and treatment groups, preventive and treatment groups were given 50 mg/kg orally of green tea extract for 14 days before and after inoculation (as prevention and treatment, respectively) with 5×108 CFU/mL H. pylori suspension orally twice daily for 7 days. Anti-peptic ulcer activity was evaluated based on the pH of gastric juice, ulcer score and body weight of rats. The results demonstrated that the use of green tea for prevention or treatment showed a significant difference (p<0.001) in reducing the pH of gastric juice 3.88 ± 0.09 and 5.74 ± 0.08, respectively, compared to vehicle control 5.85 ± 0.12. Meanwhile, vehicle control rats had high significantly reduced body weight when compared with all groups treated. The preventive and the treatment groups showed significantly reduce in ulcer score to 0.50 ± 0.18 (p<0.01) and 0.83 ± 0.10 (p<0.05), respectively, compared to vehicle control 1.50 ± 0.22. Meanwhile, it was comparable to the positive control. In conclusion, H. pylori suspension 5×108 CFU/mL given orally twice daily for seven days able to induce ulcer in Sprague Dawley rats, and the green tea extract 50 mg/kg was comparable to the standard regimen as anti-peptic ulcer agent.

 

Keywords: Anti-peptic ulcer; Camellia sinensis; green tea extract; Helicobacter pylori; ulcer score

 

ABSTRAK

Kajian ini dijalankan untuk menilai aktiviti anti-ulser peptik ekstrak etanol Camellia sinensis (teh hijau) yang diaruhkan oleh Helicobacter pylori pada tikus Sprague Dawley. Regimen piawai agen anti-ulser peptik, klaritromisin 30 mg/kg, amoksisilin 30 mg/kg dan esomeprazol 3 mg/kg digunakan sebagai kawalan positif. Tikus dibahagikan kepada 6 kumpulan (n = 6), kawalan normal, kawalan pembawa (2% suspensi tragacanth), kawalan positif untuk kumpulan pencegahan dan rawatan, kumpulan pencegahan dan rawatan yang diberikan ekstrak teh hijau 50 mg/kg secara oral selama 14 hari sebelum atau selepas inokulasi (sebagai kumpulan pencegahan dan rawatan) berserta dengan pemberian ampaian 5×108 CFU/mL H. pylori secara oral sebanyak dua kali sehari selama 7 hari. Aktiviti anti-ulser peptik dinilai berdasarkan pH jus gastrik, skor ulser dan berat badan tikus. Keputusan menunjukkan bahawa penggunaan teh hijau untuk pencegahan atau rawatan menunjukkan perbezaan yang signifikan (p<0.001) dalam mengurangkan pH jus gastrik 3.88 ± 0.09 dan 5.74 ± 0.08, setiap satu, berbanding dengan kawalan pembawa 5.85 ± 0.12. Sementara itu, terdapat penurunan berat badan yang ketara dalam tikus kawalan pembawa apabila dibandingkan dengan semua kumpulan yang dirawat. Kumpulan pencegahan dan rawatan menunjukkan penurunan skor ulser menjadi 0.50 ± 0.18 (p<0.01) dan 0.83 ± 0.10 (p<0.05), berbanding kawalan pembawa 1.50 ± 0.22. Ia adalah setanding dengan kawalan positif. Sebagai kesimpulan, pemberian ampaian H. pylori 5×108 CFU/mL diberikan secara oral dua kali setiap hari selama tujuh hari dapat menyebabkan ulser pada tikus Sprague Dawley dan ekstrak teh hijau 50 mg/kg adalah setanding dengan regimen piawai sebagai agen anti-ulser peptik.

 

Kata kunci: Anti-ulser peptik; Camellia sinensis; ekstrak teh hijau; Helicobacter pylori; skor ulser

 

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*Pengarang untuk surat-menyurat; email: e_kumolosasi@ukm.edu.my

   

 

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