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Sains Malaysiana 49(3)(2020): 603-611 http://dx.doi.org/10.17576/jsm-2020-4903-15
Effects of Plant Oligosaccharides Derived from Dragon Fruit on Gut Microbiota in Proximal and Distal Colon of Mice (Kesan Tumbuhan Oligosakarida yang Diambil daripada Buah Naga pada Mikrobiota Usus dalam Kolon
Proksi dan Distal
Tikus) SARANYA
PEERAKIETKHAJORN1,3*, NILOBON JEANMARD1,3,
PAPATSORN CHUENPANITKIT1,3, SAKENA K-DA1,2,3,
KANRAWEE BANNOB1,2 & PISSARED KHUITUAN2,3 1Department of
Biology, Faculty of Science, Prince of Songkla
University, Hat Yai, Songkhla,
90110, Thailand 2Department of
Physiology, Faculty of Science, Prince of Songkla
University, Hat Yai, Songkhla,
90110, Thailand 3Gut Biology and
Microbiota Research Unit, Prince of Songkla
University, Hat Yai, Songkhla,
90110, Thailand Diserahkan: 19 April 2019/Diterima: 18 November 2019 ABSTRACT Prebiotic oligosaccharides are used as supplements to
improve colon health. Oligosaccharides derived from dragon fruit (DFO) are a mixture of fructo-oligosaccharides (FOS), and have prebiotic properties
that increase beneficial bacteria in vitro. This study aimed to investigate changes in gut microbiota
in the colon of mice fed a diet supplemented with DFO. Treatment groups were fed 100,
500, and 1000 mg/kg of DFO, 1000 mg/kg FOS and distilled water. The results showed that DFO did
not change the body weight of mice, but altered microbiota in the
proximal and distal colon. Populations of Blautia, Parabacteroides, and Bacteroides were
among the highest
proportions of bacteria represented after all treatments. Lactobacillus was also found in the proximal and distal
colon. Moreover, qPCR results showed that Bifidobacteria
increased in the distal colon of mice treated with 100 and 1000
mg/kg DFO for 14 days, while Lactobacilli
increased in the proximal colon of mice treated with 500 mg/kg DFO for 7 days. In contrast, Enterococci decreased
in the proximal colon of mice that were given 100, 500, and 1000
mg/kg of DFO and 1000 mg/kg of FOS for 14 days. These results suggested that DFO is capable of increasing populations of beneficial bacteria while
decreasing populations of some other bacteria. Keywords: Colon; dragon fruit; gut microbiota; prebiotic oligosaccharides ABSTRAK Oligosakarida prebiotik digunakan sebagai suplemen untuk menambahbaik kesihatan kolon. Oligosakarida yang diambil daripada buah naga (DFO) adalah campuran frukto-oligosakarida (FOS) dan mempunyai sifat prebiotik yang meningkatkan bakteria berfaedah secara in vitro. Kajian ini bertujuan
untuk mengkaji
perubahan dalam mikrobiota usus pada kolon tikus
diberi makan
diet disuplementasi dengan DFO. Kumpulan rawatan diberi makan 100, 500 dan 1000 mg/kg DFO, 1000 mg/kg FOS dan air suling. Keputusan menunjukkan DFO tidak mengubah berat tikut tetapi meminda mikrobiota pada proksimal dan kolon
distal. Populasi Blautia, Parabacteroides dan Bacteroides adalah antara perkadaran
tertinggi bakteria selepas rawatan. Lactobacillus juga ditemui pada proksimal dan kolon
distal. Tambahan lagi, keputusan qPCR menunjukkan bahawaa Bifidobacteria meningkat dalam kolon distal tikus dirawat dengan 100 dan 1000 mg/kg DFO untuk 14 hari, manakala Lactobacilli meningkat dalam kolon distal tikus dirawat dengan 500 mg/kg DFO untuk 7 hari. Secara kontra, Enterococci menurun dalam kolon distal tikus yang diberi makan
100, 500 dan 1000 mg/kg DFO serta 1000 mg/kg FOS untuk 14 hari. Keputusan ini menunjukkan
bahawa DFO berupaya untuk meningkatkan
populasi bakteria
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39. *Pengarang untuk surat-menyurat; email: saranya.pe@psu.ac.th
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