Sains Malaysiana 50(10)(2021): 3085-3094

http://doi.org/10.17576/jsm-2021-5010-21

Zingiber zerumbet (L.) Smith Hexane Crude Extract Caused DNA Damage on Leptospira spp.

(Ekstrak Mentah Heksana Zingiber zerumbet (L.) Smith Menyebabkan Kerosakan DNA pada Leptospira spp.)

FARAH WAHIDA IBRAHIM¹, NURUL AMIRAH ABDUL AZIZ¹, LATIFAH IBRAHIM², NURUL FARHANA JUFRI¹ & ASMAH HAMID¹*

¹Center for Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia

²Biotechnology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Federal Territory, Malaysia

Diserahkan: 25 Mac 2020/Diterima: 18 Februari 2021

ABSTRACT

Numerous attempts have been made to control leptospirosis by using chemoprophylaxis, but with limited success. The present study was done to investigate the antileptospiral potential of hexane, ethyl acetate and methanol extracts of Zingiber zerumbet rhizomes. The extracts were assayed for antileptospiral activity using broth microdilution method against Leptospira interrogans (serovar Batavie, Canicola, Australis) and Leptospira biflexa serovar Patoc. The Z. zerumbet hexane extract exhibited antileptospiral activity, with IC₅₀ values of 248 μg/mL against L. interrogans serovar Canicola, IC₅₀ of 125 μg/mL against L. interrogans serovar Australis, IC₅₀ of 15.63 μg/mL against L. interrogans serovar Batavie and IC₅₀ of 109 μg/mL against L. biflexa serovar Patoc. However, both ethyl acetate and methanol extracts did not show any distinct antileptospiral activity. Since the hexane extract of Z. zerumbet showed antileptospiral activity, the DNA-damaging properties of this extract were tested according to their IC₅₀ and IC₂₅ values that were specific to each serovars. The DNA-damaging properties were determined by treating the selected Leptospira spp. with the hexane extract and subjecting its DNA to electrophoresis and analysis on agarose gels. The results demonstrated that the hexane extract had DNA-damaging properties towards L. biflexa serovar Patoc and L. interrogans serovar Australis, as proven by the appearance of fragmented DNA on the gels. We conclude that the Z. zerumbet hexane extract could inhibit the growth of Leptospira spp. serovar Patoc and Australis through DNA-damaging activity and thus, could be a potential antileptospiral agent. Further studies are needed to investigate the potential of this hexane extract as an antileptospiral agent using in vivo rat models of leptospirosis.

Keywords: Antileptospiral; DNA damage; growth inhibition; hexane crude extract; Zingiber zerumbet

ABSTRAK

Banyak usaha telah dibuat untuk mengawal leptospirosis dengan menggunakan kemoprofilaksis namun kejayaannya terhad. Penyelidikan ini dijalankan untuk mengkaji potensi antileptospira bagi ekstrak kasar heksana, etil asetat dan metanol rizom Zingiber zerumbet. Ekstrak telah diuji untuk aktiviti antileptospira menggunakan kaedah mikropencairan kaldu ke atas Leptospira interogans (serovar Batavie, Canicola, Australis) dan Leptospira biflexa serovar Patoc. Ekstrak heksana Z. zerumbet menunjukkan aktivti antileptospira dengan nilai IC₅₀ pada 248 μg/mL terhadap L. interrogans serovar Canicola, IC₅₀ pada 125 μg/mL terhadap L. interrogans serovar Australis, IC₅₀ pada 15.63 μg/mL terhadap L. interrogans serovar Batavie dan IC₅₀ pada 109 μg/mL untuk L. biflexa serovar Patoc. Walau bagaimanapun, kedua-dua ekstrak etil asetat dan metanol tidak menunjukkan sebarang aktiviti antileptospira yang ketara. Oleh kerana ekstrak heksana Z. zerumbet menunjukkan aktiviti antileptospira, keupayaan merosakkan DNA bagi ekstrak ini diuji mengikut nilai IC₅₀ dan IC₂₅ khusus yang diperoleh untuk setiap serovar. Hasil kajian menunjukkan bahawa ekstrak heksana mempunyai keupayaan untuk merosakkan DNA L. biflexa serovar Patoc dan L. interrogans serovar Australis seperti yang terbukti dengan kehadiran DNA yang berfragmen pada gel. Sebagai kesimpulan, ekstrak heksana Z. zerumbet boleh menghalang pertumbuhan Leptospira spp. serovar Patoc dan Australis melalui aktiviti merosakkan DNA dan dengan itu, berpotensi sebagai agen antileptospira. Kajian lanjut diperlukan untuk mengkaji potensi ekstrak heksana sebagai agen antileptospira dalam model tikus leptospirosis secara in vivo.

Kata kunci: Antileptospira; ekstrak kasar heksana; kerosakan DNA; merencat pertumbuhan; Zingiber zerumbet

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*Pengarang untuk surat-menyurat; email: asmah0901@ukm.edu.my