The Malaysian Journal of Analytical Sciences Vol 12 No 3
(2008): 624 - 632
INFLUENCE OF SURFACTANT TYPES ON CORRELATION
OF
RETENTION FACTOR AND HYDROPHOBICITY OF
SELECTED
TRIAZOLE FUNGICIDES IN MICELLAR
ELECTROKINETIC
CHROMATOGRAPHY
Wan Aini Wan Ibrahim*, Dadan Hermawan,
Mohamed Noor Hasan
and Mohd Marsin Sanagi
Separation Science Research Group (SSRG),
Department of Chemistry,
Faculty of Science, Universiti Teknologi
Malaysia,
81310 UTM Skudai, Johor, Malaysia
*Corresponding author:
wanaini@kimia.fs.utm.my
Abstract
Application of micellar electrokinetic
chromatography (MEKC) in quantitative structure-retention relationship (QSRR)
has been studied for selected triazole fungicides. Effect of different
surfactant types and concentrations of bile salts and sodium dodecyl sulfate on
the correlation between logarithm of retention factor (log k) in MEKC and
logarithm of octanol-water partition coefficient (log Pow ) was
investigated. Five standard fungicides
(cyproconazole, bromuconazole,
epoxiconazole, bitertanol and difenoconazole) with known log P values from 2.9 to 4.3 were used for
constructing the calibration curve of log Pow against the MEKC retention factor, log k.
High correlations were observed between hydrophobicity (log Pow )
and log
k in MEKC using two bile salt surfactants viz. sodium cholate and sodium
deoxycholate and mixed bile salt systems, with squared correlation coefficient
of linear regression greater than 0.98, due to the similar hydrogen bonding
interaction patterns between bile salts MEKC systems and the octanol-water
system.
Keywords
: Surfactans, micellar electrokinetic chromatography, retention factor,
hydrophobicity, triazole fungicides,
quantitative structure-retention relationship.
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