Malaysian Journal of Analytical Sciences Vol 19 No 1 (2015): 179 – 183

 

 

 

EFFECT OF TITANIUM DIOXIDE NANOPARTICLE ADDITION TO THE SURFACE CHARGE AND STRUCTURE OF DPPC VESICLES

 

(Kesan Penambahan Nanozarah Titanium Oksida ke atas Cas Permukaan dan Struktur Vesikel DPPC)

Hur Munawar Kabir Mohd, Irman Abdul Rahman*, Ainee Fatimah Ahmad, Shahidan Radiman, Faizal Mohamed, Muhamad Samudi Yasir

 

School of Applied Physics,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

 

*Corresponding author: irman@ukm.edu.my

 

 

Abstract

Titanium dioxide nanoparticle dispersions interaction with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles is reported in this paper. DPPC- TiO₂ interactions were investigated using dynamic light scattering (DLS) and transmission electron microscopy (TEM) by focusing on the effect of the interactions on the surface charge, size and localisation of TiO₂ in DPPC- TiO₂ vesicles. It was observed that surface charge of DPPC- TiO₂ vesicles were increased from -26.0 to 12.1 mV as the TiO₂ nanoparticle concentrations increased from 0.01 to 100 mg/ml. The binding of positively charged TiO₂ nanoparticle to the zwitterionic DPPC vesicles caused the surface charge to become more positive. The size distribution, localisation and positioning of TiO₂ nanoparticles in DPPC vesicles were confirmed through TEM analysis.

 

Keywords: DPPC, vesicles, titanium dioxide, DLS, TEM

 

Abstrak

Interaksi penyerakan nanozarah titanium oksida keatas vesikel 1,2-dipalmitoyl-sn-glycero-3-phosphokolina (DPPC) dilaporkan di dalam jurnal ini. Interaksi DPPC- TiO₂ telah dikaji menggunakan kaedah penyerakan cahaya dinamik (DLS) dan mikroskop transmisi electron (TEM) dengan menumpukan kepada kesan interaksi keatas cas permukaan, saiz dan penempatan nanozarah TiO₂ di dalam vesikel DPPC- TiO_2. Didapati bahawa cas permukaan vesikel DPPC- TiO₂ meningkat daripada -26.0 kepada 12.1 mV apabila kepekatan nanozarah TiO₂ meningkat daripada 0.01 kepada 100 mg/ml. Ikatan antara nanozarah TiO₂ yang bercas positif keatas vesikel DPPC yang bersifat zwiterionik menyebabkan cas permukaan menjadi lebih positif. Taburan saiz, penempatan dan kedudukan nanozarah TiO₂ di dalam vesikel DPPC telah disahkan menngunakan kaedah analisis TEM.

 

Kata kunci: DPPC, vesikel, titanium oksida, DLS, TEM

 

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