Malaysian Journal of Analytical Sciences Vol 19 No 1 (2015): 194 – 201

 

 

 

CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF HEDYCHIUM MALAYANUM ESSENTIAL OILS

 

(Komposisi Kimia dan Aktiviti Anti-agen-pengoksidaan Minyak Pati Hedychium malayanum)

Mohammed Yahya Abdo¹, Wan Yaacob Wan Ahmad¹*, Laily Bin Din¹, Nazlina Ibrahim²

 

¹School of Chemical Sciences and Food Technology,

²School of Biosciences and Biotechnology,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

 

*Corresponding author: wanyaa@ukm.edu.my

 

 

Abstract

The leaves and stems of Hedychium malayanum were hydrodistilled using a Clevenger-type apparatus to give 0.038 and 0.009% oils, respectively. Sixteen and seven compounds which made up 98.4 and 100% of the respective oils were identified using capillary GC and GC-MS with a DB-5 column. These oils contained high amount of monoterpene hydrocarbons with β-pinene being the main compound found in the leaves (39.1%) and the stems (46.7%) followed by α-pinene (22.3%) and β-caryophyllene (13.7%) which was found in the leaf oil and 1,8-cineole (17.7%) and α-pinene (16.9%) in the stem oil. Antioxidant test by 1,1-diphenyl-2-picrylhydrazyl (DPPH) showed that the leaf oil was a stronger antioxidant (31.1%) than Trolox (28.4%), whereas the stem oil was the weakest antioxidant (23.9%). β-Carotene bleaching (BCB) in linoleic acid showed that both  oils were weaker antioxidants than butylated hydroxytoulene (96.8% inhibition). However, the stem oil (79.8%) showed a good inhibition whereas the leaf oil possessed a moderate capacity (54.7%) to prevent the oxidation by linoleic acid.

 

Keywords: Hedychium malayanum, essential oil, chemical composition, GC-GC/MS, antioxidant activity

 

Abstrak

Daun dan batang Hedychium malayanum telah dihidrosuling menggunakan radas jenis-Clevenger bagi menghasilkan 0.038 dan 0.009% minyak, masing-masingnya. Enam-belas dan tujuh sebatian yang melibatkan 98.4 and 100% bagi masing-masing minyak telah dikenal pasti menggunakan KG rerambut dan KG-SJ dengan turus DB-5. Minyak-minyak ini mengandungi amaun hidrokarbon monoterpena yang tinggi dengan β-pinena merupakan sebatian utama yang ditemui dalam daun (39.1%) dan batang (46.7%) diikuti dengan α-pinena (22.3%) dan β-kariofilena (13.7%) yang ditemui dalam minyak daun dan 1,8-sineol (17.7%) and α-pinena (16.9%) dalam minyak batang. Ujian anti-agen-pengoksidaan dengan 1,1-difenil-2-pikrilhidrazil (DPPH) menunjukkan bahawa minyak daun adalah anti-agen-pengoksidaan yang lebih kuat (31.1%) berbanding Troloks (28.4%), manakala minyak batang adalah anti-agen-pengoksidaan yang paling lemah (23.9%). Pelunturan β-karotena (PBK) dalam asid linoleik menunjukkan kedua-dua minyak adalah anti-agen-pengoksidaan yang lebih lemah daripada hydroksitoulena terbutil (96.8% perencatan). Walau bagaimanapun, minyak batang (79.8%) menunjukkan perencatan yang baik manakala minyak daun memperlihatkan kapasiti sederhana (54.7%) untuk menghalang pengoksidaan oleh  asid linoleik.

 

Kata kunci: Hedychium malayanum, minyak pati, komposisi kimia, GC-GC/MS, aktiviti anti-agen-pengoksidaan

 

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