Malaysian Journal of Analytical Sciences Vol 21 No 1 (2017): 82 - 94

DOI: http://dx.doi.org/10.17576/mjas-2017-2101-10

 

 

 

GROWTH EVALUATION OF MICROALGAE ISOLATED FROM PALM OIL MILL EFFLUENT IN SYNTHETIC MEDIA

 

(Penilaian Pertumbuhan Mikroalga Yang Telah Dipencil Dari Effluen Kilang Minyak Sawit Dalam Media Sintetik)

 

Sharifah Najiha Badar¹*, Zahira Yaakob¹, Sharifah Najiha Timmiati²

 

¹Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment

²Fuel Cell Institute

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: najihabadar@gmail.com

 

 

Received: 1 June 2016; Accepted: 8 December 2016

 

 

Abstract

Discharging waste without removing harmful compounds can lead to serious environmental and health issues. Despite of the high content of pollutants, wastewater environment can become an ideal condition for the growth of several microorganisms. Some species of microalgae have been found living in palm oil mill effluent (POME), making it suitable to be used in biological treatment. However, the ability of microalgae to grow and its composition characteristics in synthetic media are unknown. Therefore, this study evaluated the growth characteristics and biochemical productivity of Chlorella sorokiniana UKM3, Coelastrella sp. UKM4 and Chlorella sp. UMACC324, which had been previously isolated from POME. Bold’s Basal Media (BBM) was used as the medium for cultivation. The experimental works were performed in a lab-scale photobioreactor at room temperature under continuous illumination for 14 days. From this study, Coelastrella sp. UKM4 showed the highest value of growth rates (0.52±0.03 d^-1), biomass productivity (0.07±0.02 g.L^-1.d^-1), lipid content (66.42±2.87 mg.L^-1, 7.26%) and lipid productivity (452.42 mg.L^-1.d^-1), whereas the highest chlorophyll content was observed in Chlorella sorokiniana UKM3 (26.84±1.76 mg.L^-1).  

 

Keywords:  microalgae, growth, biomass, chlorophyll content, lipid content

 

Abstrak

Pembuangan sisa yang tidak dirawat terlebih dahulu boleh menyebabkan isu yang serius terhadap alam sekitar dan kesihatan. Walaupun kandungan bahan pencemar adalah tinggi, air sisa boleh menjadi habitat yang ideal bagi pertumbuhan sesetengah spesis mikroorganisma. Beberapa spesies mikroalga telah dijumpai hidup dalam effluen kilang minyak sawit (POME). Ini menjadikan spesis mikroalga tersebut sesuai digunakan dalam proses rawatan sisa secara biologikal. Walau bagaimanapun, keupayaan mikroalga untuk bertumbuh dan ciri-ciri komposisinya didalam media sintetik adalah tidak diketahui. Oleh itu, kajian ini dijalankan untuk menilai ciri-ciri pertumbuhan dan produktiviti biokimia bagi Chlorella sorokiniana UKM3, Coelastrella sp. UKM4 dan Chlorella sp. UMACC324, yang sebelum ini telah dipencil dari POME. Media Bold’s Basal (BBM) telah digunakan sebagai medium pertumbuhan. Kajian ini telah dilakukan dalam fotobioreaktor berskala-makmal pada suhu bilik dan dibawah pencahayaan yang berterusan selama 14 hari. Daripada hasil kajian ini, Coelastrella sp. UKM4 telah menunjukkan nilai yang tertinggi dalam kadar pertumbuhan (0.52±0.03 hari^-1), produktiviti biojisim (0.07±0.02 g.L^-1 hari^-1), kandungan lipid (66.42±2.87 mg.L^-1, 7.26%) dan produktiviti lipid (452.42 mg.L^-1.hari^-1). Kandungan klorofil yang paling tinggi pula dapat dilihat pada Chlorella sorokiniana UKM3 (26.84±1.76 mg.L^-1).

 

Kata kunci:  mikroalga, pertumbuhan, biojisim, kandungan klorofil, kandungan lipid

 

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