Malaysian Journal of Analytical Sciences Vol 22 No 6 (2018): 1090 - 1101

DOI: 10.17576/mjas-2018-2206-20

 

 

 

EXTRACTION OF SUCCINIC ACID FROM REAL FERMENTATION BROTH BY USING EMULSION LIQUID MEMBRANE PROCESS

 

(Pengekstrakan Asid Suksinik daripada Larutan Penapaian Sebenar dengan Menggunakan Proses Membran Cecair Emulsi)

 

Norasikin Othman^1,2*, Norela Jusoh¹, Mariah Syafiqah Mohar¹, Muhammad Bukhari Rosly¹, Norul Fatiha Mohamed Noah¹

 

¹Department of Chemical Engineering, Faculty of Chemical and Energy Engineering

²Centre of Lipids Engineering and Applied Research, Ibnu Sina Institute for Scientific and Industrial Research

Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

 

*Corresponding author:  norasikin@cheme.utm.my

 

 

Received: 2 April 2018; Accepted: 31 July 2018

 

 

Abstract

Succinic acid is listed as one of the twelve building block chemicals based on the ease of production through a biotechnological approach and potential to derive various chemicals. The application of bio-based succinic acid is still limited due to high downstream processing costs. One of the potential methods to recover succinic acid is emulsion liquid membrane (ELM). The ELM system consists of three main liquid phases; external feed, membrane, and internal. In this study, the membrane phase was prepared by dissolving Amberlite LA2 as a carrier, sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) as surfactants in commercial grade palm oil, while the internal phase comprised of sodium carbonate solution, Na₂CO₃. The influence of emulsifying time, agitation speed, and agitation time on the water-in-oil-in-water (W/O/W) emulsion stability were studied. The most stable condition was implemented on various external phase concentrations to study the extraction and recovery performances. The results showed that the most stable emulsion was obtained at 5 minutes of emulsifying time, 300 rpm of agitation speed and 3 minutes of agitation time. The emulsion produced was able to provide a balanced result between stability and ELM performance as it was able to extract almost 100% of succinic acid with 98% recovery. The finding of this study showed ELM process as the potential technology to extract succinic acid produced from fermentation.

 

Keywords:  succinic acid, fermentation broth, emulsion liquid membrane, stability, extraction

 

Abstrak

Asid suksinik telah disenaraikan sebagai salah satu daripada dua belas bahan kimia asas berdasarkan kepada kemudahan pengeluaran melalui pendekatan bioteknologi dan berpotensi untuk menerbitkan pelbagai bahan kimia. Penggunaan asid bio-suksinik masih terhad disebabkan oleh kos pemprosesan hiliran yang tinggi. Salah satu daripada kaedah yang berpotensi untuk memperoleh asid suksinik ialah proses membran cecair emulsi (ELM). Sistem ELM terdiri daripada tiga fasa cecair utama; suapan luaran, membran, dan dalaman. Dalam kajian ini, fasa membran telah disediakan dengan melarutkan pembawa Amberlite LA2, surfaktan Span 80 dan Tween 80 di dalam minyak kelapa sawit komersial, manakala fasa dalaman terdiri daripada larutan natrium karbonat, Na₂CO₃. Kesan masa pengemulsian, kelajuan pengadukan, dan masa pengadukan ke atas kestabilan air-dalam-minyak-dalam air (W/O/W) telah dikaji. Keadaan emulsi yang paling stabil telah digunakan pada pelbagai kepekatan fasa luaran untuk mengkaji prestasi pengekstrakan dan perolehan. Keputusan menunjukkan keadaan emulsi yang paling stabil didapati pada 5 minit masa pengemulsian, pada 300 rpm kelajuan pengadukan, dan 3 minit masa pengadukan. Emulsi yang dihasilkan dapat memberikan hasil yang seimbang di antara kestabilan dan prestasi ELM kerana ia dapat mengekstrak hampir 100% asid suksinik dengan perolehan sebanyak 98%. Hasil daripada kajian ini menunjukkan bahawa proses ELM adalah teknologi yang berpotensi untuk mengekstrak asid suksinik yang dihasilkan daripada proses penapaian.

 

Kata kunci:  asid suksinik, larutan penapaian, membran cecair emulsi, kestabilan, pengekstrakan

 

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