Sains Malaysiana 51(8)(2022): 2415-2424

http://doi.org/10.17576/jsm-2022-5108-06

 

Hot Water Extract from Saccharomyces cerevisiae Scavenges DPPH and Reduces Senescence Associated b-Galactosidase (Sa-b-Gal) in Human Dermal Fibroblasts

(Ekstrak Air Panas daripada Saccharomyces cerevisiae Menghimpun DPPH dan Mengurangkan b-Galaktosidase (Sa-b-Gal) Berkait Senesensdalam Fibroblas Dermal Manusia)

 

KHAIZURIN TAJUL ARIFIN*, NOOR IKHWAN SHANSUDDIN, NORWAHIDAH ABDUL KARIM & SUZANA MAKPOL

 

 Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, 56000 Kuala Lumpur, Federal Territory, Malaysia

 

Received: 27 August 2020/Accepted: 25 February 2022

 

Abstract

Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HDF) cell culture. We chronicled the development of the extraction method and the subsequent biochemical assays. We used two extraction methods which were hot water extraction and rapid spin. The optimum duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which also proved that the extracts exhibited antioxidant activity. Hot water extract showed a higher antioxidant activity, and not toxic to HDF. When subjected to senescence-associated β-galactosidase (SA-β-Gal) assay, the hot water extract significantly reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 < population doubling < 40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot water extract possessed antioxidant activity by scavenging DPPH, and anti-senescent activity by reducing the expression of SA-β-Gal in pre-senescent and senescent HDF.

 

Keywords: Ageing; DPPH; Saccharomyces cerevisiae; senescence

 

Abstrak

Ekstrak daripada Saccharomyces cerevisiae digabungkan dalam banyak produk kosmetik di pasaran. Ia dikatakan mempunyai kesan yang baik pada kulit manusia, tetapi masih kurang laporan saintifik tentang keberkesanan ekstraknya. Kami telah menguji kesan ketoksikan dan aktiviti anti-penuaan bagi ekstrak mentah daripada S. cerevisiae pada model in vitro, kultur sel fibroblas dermal manusia (HDF). Kami melaporkan pembangunan kaedah pengekstrakan dan asai biokimia seterusnya. Kami menggunakan dua kaedah pengekstrakan iaitu pengekstrakan air panas dan putaran cepat. Tempoh dan fasa pertumbuhan optimum untuk menuai S. cerevisiae ditentukan dengan asai 2,2-difenil-1-pikrilhidrazil (DPPH), yang juga membuktikan bahawa ekstrak mentah menunjukkan aktiviti antioksidan. Ekstrak air panas menunjukkan aktiviti antioksidan yang lebih tinggi dan tidak toksik kepada HDF. Apabila dilakukan asai β-galaktosidase berkait penuaan (SA-β-Gal), ekstrak air panas mengurangkan ekspresi SA-β-Gal dalam HDF pra-senesens (pasaj 20, 30 < populasi berganda < 40) dan senesens (pasaj 30, populasi berganda > 50). Kesimpulannya, ekstrak air panas S. cerevisiae mempunyai aktiviti antioksidan dengan menghimpun DPPH dan aktiviti anti-senesens dengan mengurangkan ekspresi SA-β-Gal dalam HDF pra-senesens dan senesens.

 

Kata kunci: DPPH; penuaan; Saccharomyces cerevisiae; senses

 

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*Corresponding author; email: khaizurin.tajul.arifin@ppukm.ukm.edu.my

 

 

 

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