Sains Malaysiana 40(10)(2011): 1049–1052

 

Histopathology of Marine and Freshwater Fish Lymphocystis

Disease Virus (LCDV)

(Histopatologi Virus Penyakit Limfosistis (LCDV) pada Ikan Marin dan Ikan Air Tawar)

 

Mosharrof Hossain

Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

 

Myung-Joo Oh

Division of Food Science and Aqualife Medicine, Chonnam National University,

Chonnam-550-749, Korea

 

Diserahkan: 7 September 2010/Diterima: 31 Januari 2011

 

ABSTRACT

Lymphocystis disease (LCD) in fishes is caused by the agent called lymphocystis disease virus (LCDV). LCDV is a chronic and benign virus. The disease affects 96 species of marine and fresh water fishes ranged among 34 families in the world. Affected fish with LCD has a typical external symptom with clusters consisted of enormously hypertrophied dermal cells on the skin and fins. The hypertrophied cells, generally named lymphocystis cells, have a thick hyaline capsule, an enlarged nucleus and prominent basophilic cytoplasmic inclusions. Among the four species of fishes, olive flounder Paralichthys olivaceus, and rockfish Sebastes schlegeli were marine cultured fish, and gouramiTrichogaster leeri and painted glassfish Channa baculis were freshwater ornamental fish. Although LCD causes low mortality, the disfigurement of infected fish can make them unsellable. Thus LCD has resulted in an important economic loss in the aquaculture industry. This study of histopathology may be adequate for a presumptive diagnosis of lymphocystis diseases both in marine and freshwater fish species.

 

Keywords: Fish; histopathology; LCDV; lymphocystis disease; virus

 

ABSTRAK

Penyakit limfosistis (LCD) pada ikan disebabkan oleh satu agen yang disebut virus penyakit limfosistis (LDCV). LCDV merupakan satu virus kronik dan kurang bahaya. Penyakit ini memberi kesan kepada 96 spesies ikan marin dan ikan air tawar di dunia yang tergolong dalam 34 famili. Ikan yang terjangkit LCD mempunyai simptom luaran yang tipikal dengan kelompok sel dermis hipertrofi pada kulit dan sirip. Sel hipertrofi juga dikenali sebagai sel limfosistis yang mempunyai kapsul hialin tebal, nukleus yang besar dan sitoplasma basofilik. Antara empat spesies ikan kajian, Paralichthys olivaceus dan Sebastes schlegeli adalah ikan marin kultur manakalaTrichogaster leeri dan Channa baculis adalah ikan air tawar hiasan. Walaupun LCD membawa kepada mortaliti yang rendah, tetapi ikan terjangkit mempunyai bentuk cacat yang menyebabkan ia tidak dapat dijual. Oleh itu, LCD telah membawa kerugian ekonomi dalam industri akua-kultur. Kajian histopatologi ini mungkin sudah cukup untuk diagnosis presumptif ke atas penyakit limfosistis pada kedua-dua ikan marin dan air tawar.

 

Kata kunci: Histopalogi, ikan LCDV; limposistis; virus

RUJUKAN

Alonso, M.C., Cano, I., Garcia-Rosado, E., Castro, D., Lamas, J., Barja, J.L. & Borrego, J.J. 2005. Isolation of lymphocystis disease virus (LCDV) from sole (Solea senegalensis) and black spot sea bream (Pagellus bogaraveo). J. Fish Dis. 28: 221-228.

Cano, I., Alonso, M.C., Garcia-Rosado, E., Rodriguez Saint-Jean, S., Castro, D. & Borrego, J.J. 2006. Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head sea bream (Sparus aurataL.) using an immunoblot technique. Vet. Microbiol. 113: 137-141.

Dunbar, C.E. & Wolf, K. 1966. The cytological course of experimental lymphocystis in the bluegill. J. Infec. Dis. 116: 466-472.

Heppel, J. & Berthiaume, L. 1992. Ultra-structure of lymphocystis disease virus (LCDV) as compared to frog virus 3(FV3) and chilo iridescent virus (CIV): effects of enzymatic digestions and detergent degradations. Arch. Virol. 125: 215-226.

Hyatt, A.D., Gould, A.R., Zupanovic, Z., Cunnigham, A.A., Hengstberger, S., Whittngton, R.J., Kattenbelt, J. & Coupar, B.E.H. 2000. Comparative studies of piscine and amphibian iridoviruses. Arch. Virol. 145: 301-331.

Iwamoto, R., Hasegawa, O., LaPatra, S.E. & Yosumizu, M. 2002. Isolation and characterization of the Japanese flounder (Paralichthys olivaceus) lymphocystis disease virus. J. Aquat. Animal Health, 14: 114-123.

Midlege, F.H. & Malsberger, R.C. 1968. In vitro morphology and maturation of lymphocystis virus. J. Virol. 2: 830-835.

Mosharrof Hossain, Jung, S.J., Kim, W.S., Kim, S.R. & Oh, M.J. 2009. Comparison of lymphocystis disease virus proteins between marine and freshwater fish. J. Fish Pathol. 22(2): 49-53.

Oh, M.J., Kim, W.S., Kitamura, S.I., Lee, H., Son, B.W., Jung, T.S. & Jung, S.J. 2006. Change of pathogenecity on olive flounder, Paralichthys olivaceus by co-infection of Vibrio harveyi, Edwardsiella tardaand marine birnavirus. Aquaculture, 257:156-160.

Perez-Prieto, S.I., Rodriguez-Saint-Jean, S., Garsia-Rosado, E., Castro, D., Alvarez, M.C. & Borrego, J.J. 1999. Virus susceptibility of the fish cell line SAF-1 derived from gilt-head seabream. Dis. Aquat. Org. 35: 149-153.

Qin, W.Q., Gin, H.Y.H., Lee, L.Y., Gedaria, A.I. & Zhang, S. 2006. Development of cytometry based method for rapid and sensitive detection of a novel marine fish iridovirus in cell culture. J. Virol. Method. 125: 49-54.

Sheng, X.Z. & Zhan, W.B. 2006. Histopathological studies on the target organs of lymphocystis disease virus of fish. J. Ocean Univ. China 36: 749-753.

Tanaka, M., Yoshimizu, M., Kusakari, M. & Kimura, T. 1984. Lymphocystis disease in kurosoi Sebastes schlegeliand hirami Paralichthys olivaceusin Hokkaido, Japan. Bull. Japanese Soc. Sci. Fish. 50: 37-42.

Walker, D.P. & Hill, B.J. 1980. Studies on the culture assay of infectivity and some in vitro properties of lymphocystis virus. J. General Virol. 51: 385-395.

Wolf, K. 1966. The fish viruses. Adv. Virus Res. 12:36-101.

Wolf, K. 1988. Lymphocystis disease. In Fish Viruses and Fish Viral Diseases, edited by Wolf K., Ithaca: Cornell University Press.

Zhang, Q.Y., Ruan, H.M., Li, Z.Q., Yuan, X.P. & Gui, J.F. 2003. Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceusin grass carp cell lines. Dis. Aquat. Org. 57: 27-34.

Zwillenberg, L.D. & Wolf, K. 1968. Ultrastructure of lymphocystis virus. J. Virol. 2: 393-399.

 

 

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