Sains Malaysiana 50(7)(2021): 1959-1970

http://doi.org/10.17576/jsm-2021-5007-11

 

Potential of Hibiscus sabdariffa Linn. Polyphenol-Rich Extract in Improving Diabetes-Induced Vascular Functional and Structural Abnormalities in Rats

(Potensi Ekstrak Hibiscus sabdariffa Linn. Kaya Polifenol Membaikpulih Ketaknormalan Fungsi dan Struktur Vaskular Aruhan Diabetis pada Tikus)

 

FATIN FARHANA JUBAIDI1, SATIRAH ZAINALABIDIN2, LAI YING1, NUR LIYANA MOHAMMED YUSOF1 & SITI BALKIS BUDIN1*

 

1Center for Diagnostic, Therapeutic and Investigative Studies (CODTIS), Faculty of Health Science, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

2Centre for Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur, Federal Territory, Malaysia

 

Diserahkan: 14 September 2020/Diterima: 2 December 2020

 

ABSTRACT

Hibiscus sabdariffa Linn. contains a high concentration of polyphenolic compounds and shows potentials in reducing vascular complications in diabetes mellitus (DM) patients. This study was aimed to determine the ability of H. sabdariffa Linn. polyphenol-rich extract (HPE) in improving vascular endothelial dysfunction and attenuating oxidative stress in type 1 DM. DM was induced in adult male rats and the rats were then divided into three groups; untreated DM (DM); DM with HPE supplementation (DM+HPE); and DM with metformin (DM+MET). Another group of non-diabetic rats served as the normal control group. These rats were left untreated for four weeks before being subjected to the supplementations for another four weeks. The thoracic descending aorta was isolated from the treated and untreated rats to measure the vascular reactivity, oxidative stress, and morphological alterations. The results showed that HPE supplementation significantly reduced the systolic blood pressure (SBP) and mean arterial pressure (MAP) in the DM+HPE group (p<0.05). HPE also showed a tendency to improve endothelium-dependent vasorelaxation compared to the untreated diabetic rats. Rats treated with HPE exhibited a considerable improvement in the activities of antioxidants and significantly attenuated oxidative damage (p<0.05). Histological findings showed that HPE supplementation improved morphological changes in the aorta. In conclusion, HPE supplementation reduces vascular abnormalities in DM condition probably via amelioration of oxidative stress.

 

Keywords: Antioxidants; contractile response; oxidative stress; relaxation response; roselle

 

ABSTRAK

Hibiscus sabdariffa Linn. mengandungi sebatian polifenol yang tinggi dan menunjukkan potensi dalam mengurangkan komplikasi vaskular dalam keadaan diabetes melitus (DM). Kajian ini bertujuan untuk mengenal pasti sama ada ekstrak kaya polifenol H. sabdariffa Linn. (HPE) berupaya untuk meningkatkan fungsi vaskular serta membaikpulih tekanan oksidatif dalam DM jenis 1. DM diaruh pada tikus jantan dewasa dan kemudiannya tikus-tikus ini dibahagikan kepada tiga kumpulan: DM tanpa rawatan (DM); DM dengan suplementasi HPE (DM+HPE); dan DM dengan rawatan metformin (DM+MET). Satu kumpulan tikus normal dijadikan kumpulan kawalan normal. Kesemua tikus ini dibiarkan tanpa apa-apa rawatan selama empat minggu sebelum rawatan mula diberi kepada setiap kumpulan selama empat minggu berikutnya. Aorta toraks menurun diasingkan untuk pengukuran reaktiviti vaskular, tekanan oksidatif dan perubahan morfologi. Hasil kajian mendapati suplementasi HPE didapati menurunkan tekanan darah sistolik (SBP) dan purata tekanan arteri (MAP) secara signifikan (p<0.05) dan menunjukkan kecenderungan untuk memulihkan vasorelaksasi endotelium berbanding tikus DM. Suplementasi HPE turut menunjukkan peningkatan pada aras aktiviti antioksidan di samping memulihkan tekanan oksidatif secara signifikan (p<0.05). Perubahan morfologi aorta juga dipulihkan dengan suplementasi HPE. Kesimpulannya, suplementasi HPE didapati berupaya mengurangkan disfungsi ketaknormalan dalam keadaan DM mungkin melalui pemulihan tekanan oksidatif.

 

Kata kunci: Antioksidan; rosel; tekanan oksidatif; tindak balas mengecut; tindak balas relaksasi

 

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