Sains Malaysiana 50(8)(2021): 2319-2328

http://doi.org/10.17576/jsm-2021-5008-15

 

Comparing the Effects of Alpha-Tocopherol and Tocotrienol Isomers on Osteoblasts hFOB 1.19 Cultured on Bovine Bone Scaffold

(Perbandingan Kesan Isomer Alfa-Tokoferol dan Tokotrienol pada Osteoblas hFOB 1.19 yang Dikultur atas Perancah Tulang Bovin)

 

NUR FARHANA MOHD FOZI1, JAMES JAM JOLLY1, CHUA KIEN HUI2, EKRAM ALIAS3, CHIN KOK YONG1 & IMA NIRWANA SOELAIMAN1*

 

1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

2Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

3Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

Diserahkan: 3 April 2020/Diterima: 9 Disember 2020

 

ABSTRACT

Tocotrienol mixtures have been shown to exert anabolic actions on the skeletal system in animal studies, but it is unclear which tocotrienol isomer shows the most prominent effects. This study aims to investigate the most active tocotrienol isomers using hFOB 1.19 human osteoblasts cultured on a bovine bone scaffold. The bovine trabecular bone was sectioned, demineralised and freeze-dried to form the scaffold. hFOB 1.19 osteoblasts were cultured on the bone scaffolds in humidified condition at 37 °C and 5% carbon dioxide with vitamin E isomers (alpha-, beta-, gamma-, delta-tocotrienol and alpha-tocopherol). The cell differentiation capacity of tocotrienol isomers was investigated through morphological observation, alkaline phosphatase (ALP) activity and osteocalcin expression. Changes in the bone scaffolds were determined using histomorphometry methods. Osteoblast culture treated with gamma- and delta-tocotrienols showed a significant increase in ALP activity and osteocalcin expression. Bone structural histomorphometry analysis showed that bone scaffolds treated with gamma- and delta-tocotrienol showed significant increases in bone volume and trabecular thickness. In conclusion, gamma- and delta-tocotrienol show the most prominent bone anabolic effects by increasing osteoblast differentiation and enhancing bone microstructure.

Keywords: Bone; osteoblast; osteoporosis; tocopherol; tocotrienol

 

ABSTRAK

Campuran tokotrienol telah terbukti memberi kesan anabolik kepada sistem rangka di dalam kajian haiwan, tetapi isomer tokotrienol yang paling berkesan belum dikenal pasti. Kajian ini bertujuan mengkaji isomer tokotrienol yang paling aktif dengan menggunakan sel osteoblas manusia hFOB 1.19 yang dikultur atas tulang perancah lembu. Tulang trabekular lembu dipotong dan dikering-beku untuk membentuk perancah. Sel osteoblas hFOB 1.19 dikulturkan di atas perancah tulang dalam kelembapan 37 °C dan 5% karbon dioksida bersama isomer vitamin E (alfa-tokotrienol, beta-tokotrienol, gamma-tokotrienol, delta-tocotrienol dan alfa-tokoferol). Kapasiti pembezaan sel isomer tokotrienol dikaji melalui pemerhatian morfologi, aktiviti alkalin phosphatase (ALP) dan ekspresi osteokalsin. Perubahan struktur tulang ditentukan dengan kaedah histomorfometri. Sel osteoblas yag dirawat dengan gamma- dan delta-tokotrienol menunjukkan peningkatan aktiviti ALP dan ekspresi osteokalsin yang ketara. Tulang perancah yang dirawat dengan gamma-tokotrienol dan delta-tokotrienol mempunyai peningkatan isi padu serta ketebalan trabekular yang ketara melalui analisis histomorfometri. Secara kesimpulannya, gamma- dan delta-tokotrienol menunjukkan kesan anabolik tulang yang paling ketara dengan meningkatkan pembezaan osteoblas dan struktur seni tulang.

Kata kunci: Osteoblas; osteoporosis; tokoferol; tokotrienol; tulang

 

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*Pengarang untuk surat-menyurat; email: imasoel@ppukm.ukm.edu.my

 

       

 

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