Sains Malaysiana 51(7)(2022): 2025-2032

http://doi.org/10.17576/jsm-2022-5107-07

 

Identification of Pathogenic Fungi Causing Leaf Spot of Urtica cannabina and Malus sieversii in the Wild Fruit Forest of Tianshan Mountain, Xinjiang, China

(Pengenalpastian Kulat Patogen Menyebabkan Bintik Daun Urtica cannabina dan Malus sieversii di Hutan Buah Liar Gunung Tianshan, Xinjiang, China)

 

JIESHI TANG1,2, YU LIU2, CHI ZHANG3, CAIXIA HAN1 & HUA SHAO1,4*

 

1State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

2College of Life Sciences, Sichuan University, Chengdu, 610065, China

3Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi 276000, China

4Research Center for Ecology and Environment of Central Asia, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

 

Diserahkan: 28 November 2020/ Diterima: 12 Januari 2022

 

Abstract

Degradation of the wild apple trees in the wild fruit forest of Tianshan mountain of Xinjiang Province, China, has attracted great attention in recent years, and pathogens are believed to be an important responsible factor. We observed that Malus sieversii and its understory plant, Urtica cannabina, exhibited similar symptoms of leaf spot disease, and we suspect that they are caused by the same pathogens. DNA sequencing using ITS1 and ITS4 primers was applied to identify the pathogenic fungi from diseased leaves of U. cannabina and M. sieversii, which led to the identification of Alternaria sp. and Fusarium sp. as active pathogens causing same symptoms on leaves of both species. Our results implied that these two plants shared the same pathogenic fungi that cause leaf spot disease, and infection of the understory species U. cannabina might provide a reservoir of the pathogens which can attack M. sieversii and contribute at least in part, to the degradation of M. sieversii.

 

Keywords: Leaf spot; Malus sieversii; pathogenic fungi; rDNA-ITS; Urtica cannabina

 

Abstrak

Kemerosotan pokok epal liar di hutan buah liar gunung Tianshan di wilayah Xinjiang, China, telah menarik perhatian sejak beberapa tahun kebelakangan ini dan patogen dipercayai menjadi faktor utama yang terlibat. Kami memerhatikan bahawa Malus sieversii dan tumbuhan bawahnya, Urtica cannabina, menunjukkan simptom penyakit tompok daun yang sama dan kami mengesyaki bahawa ia disebabkan oleh patogen yang sama. Penjujukan DNA menggunakan pencetus ITS1 dan ITS4 digunakan untuk mengenal pasti kulat patogen daripada daun U. cannabina dan M. sieversii yang berpenyakit. Ini membawa kepada pengenalpastian Alternaria sp. dan Fusarium sp. sebagai patogen aktif yang menyebabkan gejala yang sama pada daun kedua-dua spesies. Hasil menunjukkan bahawa kedua-dua tumbuhan ini berkongsi kulat patogen yang sama yang menyebabkan penyakit bintik daun, dan jangkitan spesies U. cannabina mungkin menyediakan takungan patogen yang boleh menyerang M. sieversii dan sekurang-kurangnya menyumbang sebahagian kepada kemerosotan M. sieversii.

 

Kata kunci: Kulat patogen; Malus sieversii; rDNA-ITS; tompok daun; Urtica cannabina

 

RUJUKAN

Al-Hammadi, M.S., Al-Shariqi, R. & Maharachchikumbura, S.S.N. 2018. Molecular identification of fungal pathogens associated with date palm root diseases in the United Arab Emirates. Journal of Plant Pathology 7: 4-10.

Allison, T.D., Moeller, R.E. & Davis, M.B. 1986. Pollen in laminated sediments provides evidence for a Mid‐ Holocene Forest pathogen outbreak. Ecology 67: 1101-1105.

Beckstead, J., Meyer, S.E., Connolly, B.M., Huck, M.B. & Street, L.E. 2010. Cheatgrass facilitates spillover of a seed bank pathogen onto native grass species. Journal of Ecology 98: 168-177.

Carlier, J., Zapater, M.F. & Lapeyre, F. 2000. Septoria leafspot of Banana: A newly discovered disease caused by Mycosphaerella eumusae (Anamorph Septoria eumusae). Phytopathology 90: 884.

Cui, D.F., Liao, W.B. & Yang, H.J. 2006. Studies on the floristic composition and genesis of the wild fruit forest in Tianshan Mountains in China. Journal of Forestry Research 19: 555-560.

Cui, X., Liu, D. & Sun, K. 2018. Expression profiles and functional characterization of two odorant-binding proteins from the apple buprestid beetle Agrilus mali (Coleoptera: Buprestidae). Journal of Economic Entomology 111: 1420-1432.

Ding, X.M. 2007. Protecting Tianshan wild fruit forest without delay. Forestry of Xinjiang 6: 42-44.

Dwyer, G., Dushoff, J. & Yee, S.H. 2004. The combined effects of pathogens and predators on insect outbreaks. Nature 430: 341-345.

Elad, Y. & Pertot, I. 2014. Climate change impacts on plant pathogens and plant diseases. Journal of Crop Improvement 28: 99-139.

Filajdic, N. & Sutton, T.B. 1991. Identification and distribution of Alternaria mali on apples in North Carolina and susceptibility of different varieties of apples to Alternaria blotch. Plant Disease 75: 1045–1048.

Fredericks, D.N. & Relman, D.A. 1996. Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates. Clinical Microbiology Reviews 9: 18-33.

Guo, Z.Y. & Dong, J.G. 1995. The occurrence characteristics and control techniques of apple leaf spot disease. Agriculture Science Technology Development 2: 23.

Harteveld, D.O.C., Akinsanmi, O.A., Becker, M.F. & Drenth, A. 2014. Comparative fitness of Alternaria species causing leaf blotch and fruit spot of apple in Australia. Australasian Plant Pathology 43: 495-501.

Harteveld, D.O.C., Akinsanmi, O.A. & Drenth, A. 2013. Multiple Alternaria species groups are associated with leaf blotch and fruit spot diseases of apple in Australia. Plant Pathology 62: 289-297.

Hayden, H.L., Carlier, J. & Aitken, E.A.B. 2010. Population differentiation in the banana leaf spot pathogen Mycosphaerella musicola, examined at a global scale. Plant Pathology 52: 713-719.

Johnson, P., Hartson, R. & Larson, D. 2010. Diversity and disease: community structure drives parasite transmission and host fitness. Ecology Letter 11: 1017-1026.

Kodsueb, R. & Lumyong, S. 2019. Diversity of Saprobic Fungi on Magnolia garrettii: Do Collecting Sites and Seasons Affect the Fungal Community? Sains Malaysiana 48: 2437-2449.

Le, D.P. & Gregson, A. 2019. Alternaria leaf spot of cotton seedlings grown in New South Wales, Australia is predominantly associated with Alternaria alternate. Australasian Plant Pathology 48: 209-216.

Ling, B., Zheng, X. & Yan, G.R. 1998. Study on phyto-diversity and conservation of the wild fruit forest of Tianshan Mts in Xinjiang. Arid Zone Research 15: 10-13.

Liu, Y., Wei, C.X., Zhou, S.X., Tang, J.S., Han, C.X. & Shao, H. 2019. Identification of pathogenic fungi causing leaf spot of Malus sieversii and Impatiens brachycentra growing in the wild fruit forest of Tianshan Mountain. Journal of Plant Protection 46: 345-351.

Liu, L., Zhang, L., Qiu, P.L., Wang, Y., Liu, Y.N., Li, Y., Gao, J. & Hsiang, T. 2020. Leaf spot of Polygonatum odoratum caused by Colletotrichum spaethianum. Journal of General Plant Pathology 86: 157-161.

Lively, C.M., Johnson, S.G., Delph, L.F. & Clay, K. 1995. Thinning reduces the effect of rust infection on jewelweed (Impatiens capensis). Ecology 76: 1859-1862.

Lu, S.J., Gong, Z.Y. & Luan, T.M. 1984. A preliminary report on the occurrence and control of apple leaf spotted leaf disease. China Fruits 1: 38-43.

Mansur, P., Silva, A.L., Salcedo, S.S., Barreto, R.W. & Colmán, A.A. 2020. Alternaria dauci causes leaf spots and leaf blight of coriander (Coriandrum sativum) in Brazil. Australasian Plant Disease Notes 15: 38.

Menzel, A., Hempel, S. & Klotz, S. 2016. Mycorrhizal status helps explain invasion success of alien plant species. Ecology 98: 92-102.

Poletto, T., Muniz, M.F.B., Fantinel, V.S., Martello, L.D.S., Savian, L.G., Harakava, R., Guatimosim, E., Poletto, I. & Stefenon, V.M. 2021. Characterization of the brown leaf spots pathosystem in Brazilian pecan orchards: pathogen morphology and molecular identification. Annals of Forest Research 64: 75-86.

Power, A.G. & Mitchell, C.E. 2004. Pathogen spillover in disease epidemics. American Naturalist 164: S79-S89.

Qi, X.L., He, J., Li, D.E. & Huang, L. 2021. First report of leaf spot on Elaeagnus pungens caused by Epicoccum latusicollum in China. Forest Pathology 51: e12716.

Rotondo, F., Collina, M. & Pryor, B.M. 2012. Comparison of Alternaria spp. collected in Italy from apple with A. mali and other AM-toxin producing strains. Phytopathology 102: 1130-1142.

Sawamura, K. 1962. Studies on spotted disease of apples. I. Causal agent of Alternaria blotch. Bulletin of the Tohoku National Agricultural Experiment Station 23: 163-175.

Shao, X.P., Zhong, X.G., Xue, Y.Y., Liu, J., Zhang, S.W., Wang, W.X. & Xu, B.L. 2014. Identification and its cross protection on pathogens of apple leaf spot in Gansu Province. Journal of Gansu Agricultural University 49: 78-84.

Shi, T.J., Long, Z.Z. & Miao, M. 2020. Glomus mosseae promotes Xanthium italicum invasion. Sains Malaysiana 49(10): 2425-2432.

Shi, X.J., Zeng, K.X., Wang, X.Y., Liang, Z.H. & Wu, X.H. 2021. Characterization of Alternaria species causing leaf spot on Chinese cabbage in Shanxi province of China. Journal of Plant Pathology 103: 283-293.

Simon, L. & Clapp, J.P. 2008. Single-strand conformational polymorphism (SSCP) for the identification of Endomycorrhizal fungi [M]// Species Diagnostics Protocols.

Song, L.S., Lin, W., Jiang, N., Zhang, Z.J., Tan, G.Y. & Wei, S.G. 2021. Anthracnose disease of Amomum villosum caused by Colletotrichum fructicola in China. Journal of General Plant Pathology 87: 259-263.

Song, S., Zhang, Y. & Liu, H. 2019. Isolation and characterization of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial spot in Physalis pubescens in Northeast China. Journal of Plant Pathology 101: 361-366.

Tang, J.S., Huang, L., Liu, L., Toshmatov, Z., Zhang, C. & Shao, H. 2020. Two phytotoxins isolated from the pathogenic fungus of the invasive weed Xanthium italicum. Chemistry & Biodiversity 17: 23-34.

Velho, A.C., Stadnik, M.J. & Casanova, L. 2014. First report of Colletotrichum karstii causing glomerella leaf spot on apple in Santa Catarina State, Brazil. Plant Disease 98: 157.

Woudenberg, J.H.C., Truter, M. & Crous, P.W. 2014. Alternaria section Alternaria: Species, formae speciales or pathotypes? Studies in Mycology 82: 1-21.

Yang, B., Jin, X.H., Feng, Q.C., Xiao, K.L., Zhang, H.Z., Tang, T.T., Shan, L.Y. & Guo, W. 2020. Colletotrichum species causing leaf spot diseases of Liriope cymbidiomorpha (ined.) in China. Australasian Plant Pathology 49: 137-139.

Zhang, Y., Tang, J.S., Ren, G., Zhao, K.X. & Wang, X.F. 2021. Global potential distribution prediction of Xanthium italicum based on Maxent model. Scientific Reports 11: 16545.


*Pengarang untuk surat-menyurat; email: shaohua@ms.xjb.ac.cn

 

 

   

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