Sains Malaysiana 51(7)(2022): 2129-2136

http://doi.org/10.17576/jsm-2022-5107-15

 

Interleukin-1b and Interferon-g are Associated with Malaria-Induced Hypoinsulinemic Hypoglycemia in Plasmodium berghei Anka-Infected Mice

(Interleukin-1b dan Interferon-g dikaitkan dengan Hipoglisemia Hipoinsulinemik Mengaruh Malaria pada Tikus yang Dijangkiti Plasmodium berghei Anka)

 

 RUJIKORN RATTANATHAM1,2 & VORAVUTH SOMSAK1,2,*

 

1School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand

2Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat 80160, Thailand

 

Diserahkan: 17 Mei 2021/Diterima: 30 November 2021

 

Abstract

Malaria-induced hypoglycemia is recognized as a serious complication of malaria and has one of the strongest associations with mortality in children. It has been speculated that oxidative stress and pro-inflammatory response during parasite infection were involved in its pathophysiology. Hence, this study aimed to investigate the development of malaria-induced hypoglycemia during Plasmodium berghei ANKA (PbANKA) infection with particular attention to the involvement of c-peptide, interleukin-1b (IL-1b), and interferon-g (IFN-g). ICR mice were infected with 1×107 parasitized erythrocytes of PbANKA, and parasitemia was monitored, and the development of hypoglycemia was assessed by measuring plasma glucose levels. The change of c-peptide level was evaluated. The pro-inflammatory response of IL-1b and IFN-g were also quantified in plasma. It was found that PbANKA infection resulted in hypoglycemia as indicated by a significantly (P < 0.05) decrease in plasma glucose levels on day 4 post-infection and associated with parasitemia. The c-peptide was slightly increased at day 2 post-infection, and then significantly (P < 0.05) decreased since day 4. Furthermore, we observed a significantly (P < 0.05) increased IL-1b, firstly responded, at day 2 post-infection followed by increasing the IFN-g level at day 4 in PbANKA-induced hypoglycemia. Our findings support the idea that hypoinsulinemic hypoglycemia in the PbANKA infected mice may be involved in the high IL-1b and IFN-g against the parasite infection.

 

Keywords: Hypoglycemia; IFN-g; IL-1b; malaria; Plasmodium berghei

 

Abstrak

Hipoglisemia yang disebabkan oleh malaria dikenali sebagai komplikasi malaria yang serius dan mempunyai salah satu kaitan paling kuat dengan kematian yang berlaku dalam kalangan kanak-kanak. Spekulasi bahawa tekanan oksidatif dan tindak balas pro-radang semasa jangkitan parasit terlibat dengan patofisiologinya. Oleh itu, kajian ini bertujuan untuk mengkaji perkembangan hipoglikemia yang disebabkan oleh malaria semasa jangkitan Plasmodium berghei ANKA (PbANKA) dengan perhatian khusus dilakukan terhadap penglibatan c-peptida, interleukin-1b (IL-1b) dan interferon-g (IFN-g). Tikus ICR telah dijangkiti dengan 1×107 eritrosit parasit PbANKA dan parasitemia dipantau dan perkembangan hipoglikemia dinilai dengan mengukur tahap glukosa pada plasma. Perubahan tahap c-peptida ini dinilai. Tindak balas pro-radangIL-1b dan IFN-g juga dikira pada plasma. Jangkitan PbANKA didapati telah mengakibatkan hipoglikemia dan berlakunya penurunan ketara (P <0.05) dalam paras glukosa plasma pada hari ke-4 selepas jangkitan dan dikaitkan dengan parasitemia. C-peptida meningkat sedikit pada hari ke-2 selepas jangkitan dan kemudian menurun dengan ketara (P <0.05) pada hari ke-4. Seterusnya peningkatan ketara (P <0.05) IL-1b berlaku, yang mula bertindak balas, pada hari ke-2 selepas jangkitan diikuti dengan meningkatkan tahapIFN-g pada hari ke-4 dalam hipoglikemia yang disebabkan oleh PbANKA. Penemuan kajian menyokong idea bahawa hipoglikemia hipoinsulinemia pada tikus yang dijangkiti PbANKA mungkin terlibat dalam peningkatanIL-1b dan IFN-g terhadap jangkitan parasit.

 

Kata kunci: Hipoglisemia; IFN-g; IL-1b; malaria; Plasmodium berghei

 

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*Pengarang untuk surat-menyurat; email: voravuth.so@wu.ac.th

 

 

   

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