Sains Ma1aysiana 28: 119-126 (1999)                                                                          Sains Fizis dan Gunaan/

                                                                                                                                                Physical and Applied Science


The Use of Solid Phase Microextraction Technique for the

Extraction of Trihalomethanes in Drinking Water



Md. Pauzi Abdullah & Aiman M. Bobaker

Jabatan Kimia, Fakulti Sains Fizis dan Gunaan

Universiti Kebangsaan Malaysia

43600 UKM Bangi Selangor D.E. Malaysia





Solid phase micro-extraction technique is a fast, sensitive, inexpensive, portable and solvent free method for extracting organic compounds from aqueous samples. This technique involves exposing a fused silica fibre that has been coated with a stationary phase to an aqueous solution containing organic contaminants. The analytes partition into the stationary phase until an equilibrium has been reached, after which the fibre is removed from the solution and the analytes are thermally desorbed into the injector of a gas chromatograph. Optimization work were carried out for the time of absorption and desorption, and the equilibrium temperatures for the extraction of trihalomethanes in drinking water samples. Almost 100% recovery was achieved within three minutes of equilibration between 25 to 30°C with desorption time of two minutes. The samples of drinking water supplies were analyzed against calibrated standard solutions in deionized water. With 20.0 ml water sample, the method's detection limit MDL of 0.01 ppb was achieved for CHCl3 and CHBrCI2. For CHBr2Cl and CHBr3 the MDL were 0.04 and 0.06 ppb, respectively.




Teknik pengekstrakan mikro fasa pepejal ialah satu teknik pengekstrakan sebatian organik yang cepat, peka, murah dan bebas pelarut. Teknik ini melibatkan pencelupan gentian silika terlakur yang dilapisi dengan suatu fasa pegun ke dalam larutan yang mengandungi pencemar organik. Analit organik akan terjerap kepada gentian sehingga mencapai keseimbangan. Gentian yang mengandungi organik terjerap dikeluarkan daripada larutan dan kemudiannya dinyahjerap di dalam penyuntik alat kromatografi gas. Teknik ini telah dioptimumkan dari segi masa jerapan, masa nyahjerapan dan suhu keseimbangan. Hampir 100% pemulihan dicapai dengan masa keseimbangan selama 3 minit pada suhu antara 25°C ke 30°C dan dengan masa nyahjerap selama 2 minit. Analisis ke atas beberapa sampel air minum telah dilakukan menggunakan teknik ini. Bagi isipadu sampel sebanyak 20.0 ml, had pengesanan kaedah serendah 0.01 ppb telah dicapai bagi komponen CHCI3 dan CHBrCI2. Bagi CHBr2Cl dan CHBr3, had pengesanan kaedah masing-masingnya ialah 0.04 dan 0.06 ppb.





Arthur C, Pratt K., Motlagh S., & Pawliszyn J. 1992. Environmental analysis of organic compounds in water using solid phase micro extraction. J High Res Chromat. 15: 741-744.

Attas L., Contu A., Loizzo A., Massiglia M., Valente P. & Zapponi G. A. 1995. Trihalomethanes in drinking water and cancer: risk assessment and integrated evalution of available data, in animals and humans. Science of the Total Environment. 171: 61-68.

Boyd-Boaland A.A., Chai M., Luo Y. Z., Zhang Z., Yang M. J. & Pawliszyn J. 1994. New solvent-free sample preparation techniques based on fibre and polymer technologies, Environ. Sci. Technol. 28: A569-A574.

Cotruvo, J. A. & Wu C. 1981. Controlling organics: Why now? J. AWWA. 70 (11): 590-594. 

USEPA, 1998. Appendix A: National Primary Drinking Water Standards. United States Environmental Protection Agency. 

Louch D., Safa Motlagh and Pawliszyn, J. 1992. Dynamics of organic compound     extraction from water using liquid-coated fused silica fibers. Anal. Chem. 64: 1187-1199.

Oliver & Lawrence J. 1979. Haloforms in drinking water: A study of precursors and precursor removal. J. AWWA. 71 (3): 161-163.     

Otson R., Williams D.T. & Bigg D.C. 1982. Relationship between raw water quality, treatment and occurance of organics in Canadian potable water. Bull Environ. Contam. Toxicol. 28 : 396-403.

Peter, C.J. & Perry, R. 1980. The formation and control of trihalomethanes in water treatment processes. Water Sci .& Technol. 13 (1-4): 103-122.

Potter D. W. & Pawliszyn J. 1992. Detection of substituted benzenes in water at the pg/ml level using solid-phase microextraction and gas chromatography - ion trap mass spectrometry, J. Chromatogr. 625: 247

Rook, J. J. 1974. Formation of haloforms during chlorination of natural water. Water Treatment & Exam. 23: 234

Rook, J.J. 1976. Haloforms in drinking water. J. AWWA. 68: 168-172.

APHA 1992. Standard Methods for the Examination of Water and Wastewater. 18th ed. APHA, AWWA and WEF. Washington DC.

White, G.c. 1986. Handbook of Chlorination. 2nd. Ed. New York: Van Nostrand Reinhold Co.,

World Health Organization. 1984. Guidelines for Drinking Water Quality 2:    242-245.

Zhang, M.J. Yang & Pawliszyn J. 1994. Solid-phase microextraction. Anal. Chem. 66: A844-A853.