Sains Malaysiana 44(11)(2015): 1587–1591

Inhibition of Consortium Sulfate Reducing Bacteria from Crude Oil for Carbon Steel Protection

(Perencatan Konsortium Bakteria Penurun Sulfat daripada Minyak Mentah sebagai Pelindung Keluli Karbon)

NUR AKMA MAHAT¹*, NORINSAN KAMIL OTHMAN¹, FATHUL KARIM SAHRANI² & MOHD NAZRI IDRIS^1,3

¹School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor Darul Ehsan. Malaysia

²School of Environmental and Natural Resource Sciences, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia

³School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Pulau Pinang, Malaysia

Received: 28 March 2015/Accepted: 29 June 2015

ABSTRACT

The efficiency of cetyltrimethylammonium bromide (CTAB) to reduce the activity of consortium bacteria consisting of sulphate-reducing bacteria (C-SRB) has been investigated on variable concentration by weight loss test, potentiodynamic polarization and diffusion disk methods. C-SRB was isolated from tropical crude oil of Malaysian offshore. Biofilm analysis was also evaluated by variable pressure scanning electron microscopy (VPSEM). Weight loss and potentiodynamic polarization analyses showed that CTAB is able to inhibit the biocorrosion process and their inhibition efficiency had reached to 85 and 65% at 300 ppm CTAB, respectively. Increasing of CTAB efficiency as a function of concentration was also supported by diffusion disk analysis. Biofilm analysis showed that less of C-SRB and their metabolic by-product had been observed. It was concluded that CTAB was able to reduce the C-SRB activity and prevent biocorrosion process on carbon steel surface.

Keywords: Carbon steel; consortium SRB; potentiodynamic polarization

ABSTRAK

Kecekapan Cetriltrimetilammonium bromida (CTAB) untuk mengurangkan aktiviti bagi konsortium bakteria yang mengandungi bakteria penurun sulfat (C-SRB) telah dikaji pada kepekatan yang berbeza dengan menggunakan kaedah kehilangan berat, pengutuban potensiodinamik dan resapan cakera. SRB telah diasingkan daripada minyak mentah tropika luar pesisir Malaysia. Analisis biofilem juga dinilai dengan mikroskopi pengimbas elektron pemboleh ubah tekanan (VPSEM). Analisis kehilangan berat dan pengutuban potensiodinamik mendedahkan bahawa CTAB mampu untuk menghalang berlakunya proses biokakisan dan kecekapan perencat yang ditunjukkan bagi kedua-dua kaedah telah mencapai kepada 85 dan 65% pada 300 ppm CTAB. Peningkatan kecekapan CTAB sebagai fungsi kecekapan juga disokong oleh analisis resapan cakera. Analisis biofilem menunjukkan bahawa terdapatnya pengurangan daripada sel C-SRB dan juga hasil produk metabolik bakteria telah diperhatikan. Kesimpulannya, CTAB mampu untuk mengurangkan aktiviti C-SRB dan juga mencegah daripada berlakunya proses biokakisan terhadap keluli karbon.

Kata kunci: Keluli karbon; konsortium SRB; pengutuban potensiodinamik

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*Corresponding author; email: nurakmamahat@yahoo.com