Sains Malaysiana 44(11)(2015): 1593–1598

Influence of Benzyltriethylammonium Chloride on Biocorrosion Activity of Consortium Bacteria from Tropical Crude Oil

(Pengaruh Benziltrietilamonium Klorida terhadap Aktiviti Biokakisan Konsortium Bakteria daripada Minyak Mentah Tropika)

MOHD NAZRI IDRIS^1,2*, ABDUL RAZAK DAUD¹, NUR AKMA MAHAT¹, MOHD HAFIZUDDIN AB GHANI¹, FATHUL KARIM SAHRANI² & NORINSAN KAMIL OTHMAN¹

¹School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor Darul Ehsan, Malaysia

²School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Pulau Pinang, Malaysia

³School of Environment and Natural Resources Science, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia

Received: 28 March 2015/Accepted: 3 July 2015

ABSTRACT

The performance of pipeline system used in petroleum industry is crucially declined by natural microbial activities and demanding extra operational cost. Requirement on high capability of functional substances is attracting worldwide research interest. The aim of this paper was to study the effectiveness of benzyltriethylammonium chloride (BTC) on reducing the activity of a consortium bacteria consisting of sulfate-reducing bacteria (C-SRB). C-SRB was isolated from tropical crude oil and enumeration of this consortium was measured by viable cell count technique. The effectiveness of BTC was calculated from potentiodynamic polarization method and biofilm analysis was performed by scanning electron microscope. The viable cell count technique indicated that the maximum growth of C-SRB was approximately 160 trillion CFU/mL at 7 days incubation period. BTC was capable of reducing biocorrosion activity due to adsorption process and mitigating SRB species. Biofilm analysis has proven that C-SRB activity is minimized due to less presence of bacterial growth, extracellular polymeric substances and corrosion product. In conclusion, BTC is capable to inhibit C-SRB activity on biocorrosion of carbon steel pipeline.

Keywords: Carbon steel; consortium bacteria; potentiodynamic polarization

ABSTRAK

Keupayaan sistem saluran paip yang digunakan dalam industri petroleum banyak mengalami penyusutan akibat aktiviti mikrob dan memerlukan kos pengoperasian yang tinggi. Keperluan keupayaan yang tinggi daripada sebatian berfungsi telah menarik minat penyelidikan seluruh dunia. Matlamat kajian ini adalah untuk mengkaji kecekapan benziltrietilamonium klorida (BTC) terhadap penurunan aktiviti konsortium bakteria yang mengandungi bakteria penurun sulfat (C-SRB). C-SRB telah dipencilkan daripada minyak mentah tropika dan pengangkaan konsortium ini telah dihitung melalui teknik kiraan sel boleh hidup. Kecekapan BTC ditentukan daripada kaedah kekutuban keupayaan dinamik dan analisis biofilem telah dijalankan dengan menggunakan mikroskop elektron imbasan. Teknik kiraan sel boleh hidup mendapati nilai pertumbuhan maksimum C-SRB dianggarkan berjumlah 160 trillion CFU/mL dalam tempoh 7 hari pengeraman. BTC didapati berupaya mengurangkan aktiviti biokakisan melalui proses penjerapan dan pengurangan spesies SRB. Analisis biofilem membuktikan bahawa aktiviti C-SRB adalah minimum dengan penggunaan BTC disebabkan kurangnya pertumbuhan bakteria, juga sebatian polimer ekstrasel dan hasil kakisan. Sebagai kesimpulan, BTC didapati berupaya menyekat aktiviti C-SRB dan proses biokakisan pada permukaan paip keluli karbon.

Kata kunci: Kekutuban keupayaan dinamik; keluli karbon; konsortium bakteria

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*Corresponding author; email: nazriselama@gmail.com