Sains Malaysiana 46(9)(2017): 14651469


Effect of Storage Time and Concentration of Used Cooking Oil on Polyhydroxyalkanoates (PHAs) Production by Cupriavidus necator H16

(Kesan Masa Penyimpanan dan Kepekatan Minyak Masak Terpakai Terhadap Pengeluaran

Polihidroksyalkanoat melalui Cupriavidus necator H16)




1Department of Molecular Biotechnology and Bioinformatics, Faculty of Science

Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand


2Center of Excellent for Genomics & Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand


Diserahkan: 31 Ogos 2016/Diterima: 17 Januari 2017



Polyhydroxyalkanoates (PHAs) can be used to replace petrochemical plastics in many applications. However, their production has limitation due to production cost. This research was conducted using alternative carbon source from waste to synthesize PHAs by Cupriavidus necator H16. In this study, PHAs were produced from used cooking oil (UCO) and compared with production PHAs from palm oil. Two UCO storage times (4 and 10 weeks) and three UCO concentrations (10, 20 and 30 g/L) were used to determine the most optimum condition for PHA synthesis. The best optimum condition for PHA synthesis was cultivated in the medium containing 30 g/L of UCO at 4 weeks storage. The cell dry weight (CDW) and PHA content were 5.260.61 g/L and 27.362.04 wt. %, respectively. These results were similar to cell cultivation using 20 g/L palm oil and 1% of fructose (5.930.33 g/L of CDW and 26.966.14 wt. % of PHA contents). In addition, PHA content from the culture with 10 g/L of UCO stored for 10 weeks was higher than PHA content from the culture with 20 and 30 g/L of UCO. Thus, it can be concluded that UCO could be used in PHA production.


Keywords: Bioplastics; Cupriavidus necator H16; polyhydroxyalkanoates (PHAs); used cooking oil (UCO)



Polihidroksialkanoat (PHA) boleh digunakan untuk menggantikan plastik petrokimia dalam kebanyakan aplikasi. Walau bagaimanapun, pengeluarannya mempunyai had kerana kos pengeluaran. Kajian ini dijalankan menggunakan sumber karbon alternatif daripada bahan buangan untuk mensintesis PHA menggunakan Cupriavidus necator H16. Dalam kajian ini, PHAs dihasilkan daripada minyak masak terpakai (UCO) dan dibandingkan dengan pengeluaran PHA daripada minyak sawit. Dua masa simpanan UCO (dua 4 dan 10 minggu) serta tiga kepekatan UCO (10, 20 dan 30 g/L) telah digunakan untuk menentukan syarat keadaan optimum untuk sintesis PHA. Keadaan optimum terbaik untuk sintesis PHA ialah dieram pada medium yang mengandungi 30 g/L UCO pada 4 minggu penyimpanan. Berat sel kering (CDW) dan kandungan PHA masing-masing adalah 5.260.61 g/L dan 27.362.04 % bt. Keputusan ini adalah sama dengan penanaman sel menggunakan 20 g/L minyak sawit dan 1% fruktosa (5.930.33 g/L CDW dan 26.966.14 % bt. kandungan PHA). Di samping itu, kandungan PHA daripada kultur dengan 10 g/L UCO yang disimpan selama 10 minggu adalah lebih tinggi daripada kandungan PHA yang dikultur dengan 20 dan 30 g/L UCO. Oleh itu, dapat disimpulkan bahawa UCO boleh digunakan dalam pengeluaran PHA.


Kata kunci: Bioplastik; minyak masak terpakai (UCO); Necator cupriavidus H16; polihidroksialkanoat (PHA)




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