Sains Malaysiana 46(9)(2017): 1471Ė1477

http://dx.doi.org/10.17576/jsm-2017-4609-16

 

Gynura procumbens Leaf Improves Blood Glucose Level, Restores Fertility and Libido of Diabetic-Induced Male Rats

(Daun Gynura procumbens Meningkatkan Aras Glukosa Darah serta Memulihkan Kesuburan dan Libido Tikus Jantan Aruhan Diabetik)

 

KHAIDATUL AKMAR KAMARUZAMAN & MAHANEM MAT NOOR*

 

School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 31 Ogos 2016/Diterima: 1 April 2017

 

 

ABSTRACT

Gynura procumbens (GP) has been widely used in traditional medicine due to its ability in treating various types of diseases. This study was carried out to determine the effects of GP on blood glucose level, fertility and libido of streptozotocin-induced male rats. A total of 42 male rats were randomly sorted into six groups; normal, negative and positive controls and three treated groups with different dosages of GP aqueous extract (GPAE); 150, 300 and 450 mg/kg. Each group was treated via oral feeding for 14 consecutive days. The rats were sacrificed on day 15 for further analysis. Administration of GPAE significantly lowered the fasting blood glucose (FBG) level and increased the plasma testosterone level of diabetic-induced rats compared to the positive and negative control groups. There was also a significant increase in the sperm quality as well as the fertility of GPAE treated groups compared to the negative and positive control groups. Sexual behaviour analysis demonstrated that GPAE is able to facilitate the expression of sexual behaviour significantly; increased mounting frequency and reduced mounting latency. These lines of evidence suggested that GPAE can act as antihyperglycaemic, profertility and libido agents. However, its mechanism(s) remains unresolved.

 

Keywords: Diabetic; fertility; Gynura procumbens; sexual behaviour; testosterone

 

ABSTRAK

Gynura procumbens (GP) telah digunakan secara meluas dalam perubatan tradisional kerana keupayaannya dalam merawat pelbagai jenis penyakit. Kajian ini telah dijalankan untuk menentukan kesan GP ke atas aras glukosa darah, kesuburan dan libido tikus jantan yang diaruh streptozotocin. Sejumlah 42 ekor tikus jantan telah diasingkan secara rawak kepada enam kumpulan; kawalan normal, negatif dan positif serta tiga kumpulan yang dirawat dengan dos berbeza ekstrak akues GP (GPAE); 150, 300 dan 450 mg/kg. Setiap kumpulan diberi perlakuan secara oral selama 14 hari berturut-turut. Tikus dikorbankan pada hari ke-15 untuk analisis lanjut. Perlakuan GPAE dengan signifikan telah menurunkan tahap glukosa darah berpuasa (FBG) dan meningkatkan aras testosteron plasma tikus diaruh diabetes berbanding dengan kumpulan kawalan positif dan negatif. Terdapat juga peningkatan signifikan dalam kualiti sperma serta kesuburan kumpulan rawatan GPAE berbanding dengan kumpulan kawalan negatif dan positif. Kajian kelakuan seksual menunjukkan bahawa GPAE ini dapat meningkatkan kelakuan seksual dengan signifikan; meningkatkan kekerapan pemanjatan dan mengurangkan tempoh pendam pemanjatan. Bukti ini mencadangkan bahawa GPAE boleh bertindak sebagai agen anti hiperglisemik, prokesuburan dan libido. Walau bagaimanapun mekanisme ini masih belum diketahui.

Kata kunci: Diabetis melitus; Gynura procumbens; kelakuan seksual; kesuburan; testosteron

RUJUKAN

Adaikan, P.G. 2011. Abstract 17. Decline in testosterone: Relationship to libido and sexual dysfunction. Journal of Menís Health 8(Suppl. 1): S103.

Agmo, A. 1997. Protocol male rat sexual behaviour. Brain Research Protocols 1: 203-209.

Akowuah, G.A., Sadikun, A. & Mariam, A. 2002. Flavonoid identification and hypo-glycemic studies of butanol fraction from Gynura procumbens. Pharmaceutical Biology 40: 405-410.

Akbarzadeh, A., Norouzian, D., Mehrabi, M.R., Jamshidi, Sh., Farhangi, A., Allah Verdi, A., Mofidian, S.M.A. & Lame Rad, B. 2007. Induction of diabetes by Streptozotocin in rats. Indian Journal of Clinical Biochemistry 22(2): 60-64.

Algariri, K., Meng, K.Y., Atangwho, I.J., Asmawi, M.Z., Sadikun, A., Murugiyah, V. & Ismail, N. 2013. Hypoglycemic and anti-hyperglycemic study of Gynura procumbens leaf extracts. Asian Pacific Journal of Tropical Biomedicine 3(5): 358-366.

Alvarez, J.G. & Storey, B.T. 1995. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Molecular Reproduction and Development 42: 334-346.

Alvez, M.G., Martins, A.D., Vaz, C.V., Correia, S., Moreira, P.I., Oliveira, P.F. & Socorro, S. 2014. Metformin and male reproduction: Effects on Sertoli cells metabolism. British Journal of Pharmacology 171: 1032-1042

Alvez, M.G., Martins, A.D., Caraco, J.E., Socorro, S. & Oliveira, P.F. 2013a. Molecular mechanisms beyond glucose transport in diabetes-related male infertility. Biochimica et Biophysica Acta 1832: 626-635.

Alvez, M.G., Martins, A.D., Caraco, J.E., Socorro, S. & Oliveira, P.F. 2013b. Diabetes, insulin mediated glucose metabolism and Sertoli/blood-testes barrier function. Tissue Barriers 1: 1-12.

Alvez, M.G., Rato, L., Carvaiho, R.A., Moreira, P.I., Socorro, S. & Oliveira, P.F. 2013c. Hormonal control of Sertoli cells metabolism regulates spermatogenesis. Cellular and Molecular Life Sciences 70: 777-793.

Biggers, J.D., Whitten, W.K. & Whittingham, D.G. 1971. The culture of mouse embryos in vitro. In Methods in Mammalian Embryology, edited by Daniel Jr., J.C. San Francisco: W.H. Freeman. pp. 86-116.

Chauhan, A. & Agarwal, M. 2009. Assessment of the contraceptive efficacy of the aqueous extract of Aegel marmelos Corr. leaves in the male albino rats. Human Fertility 12: 107-118.

Butchi Akondi, R., Kumar, P., Annapurna, A. & Pujari, M. 2011. Protective effect of rutin and naringin on sperm quality in streptozotocin (STZ) induced type I diabetic rats. Iranian Journal of Pharmaceutical Research 10(3): 585-596.

Gaochao, Z., Myers, R., Ying, L., Chen, Y., Xiaolan, S., Judy, F.M., Margaret, W., John, V., Thomas, D., Nobuharu, F., Nicalso, M., Michael, F.H., Laurie, J.G. & David, E.M. 2001. Role of AMP-activated protein kinase in mechanism of metformin action. The Journal of Clinical Investigation 108: 1167-1174.

Gupta, A.K., Bindal, M.C., Gupta, S.K., Prakash, D.B. & Vedpal. 2013. Aphrodisiac activity of Semecarpus anacordium nut. International Research Journal of Pharmacy 4(4): 202-204.

Gupta, R.S., Kachhawa, J.B.S., Khushalani, V., Tanuar, K. & Joshi, Y.C. 2006. Effect of Cressa cretica methanol extract on testicular function of albino rats. Pharmaceuticals Biology 44: 382-388.

Khaki, A., Nouri, M., Fathiazad, F., Ahmadi-Ashtiani, H.R., Rastgar, H. & Rezazadeh, S. 2009. Protective effects of quercetin on spermatogenesis in streptozotocin-induced diabetic rats. Journal of Medicinal Plants 5(8): 57-64.

Ladachart, T., Prakong, T., Noppadon, K. & Suchinda, M. 2008. Stimulating effects of quercetin on sperm quality and reproductive organs in adult male rats. Asian Journal of Andrology 10: 249-258.

Mahanem Mat Noor & Nani Rahayu. 2012. Anti-hyperglycemic effect of Gynura procumbens methanolic extract on fertility and libido of induced diabetic male rats. Sains Malaysiana 41(12): 1549-1556.

Mita, M. & Hall, F. 1982. Metabolism of round spermatids from rats: Lactate as the preferred substrate. Biology of Reproduction 26(3): 445-455.

Oonk, R.B. & Grootegoed, J.A. 1987. Identification of insulin receptors on rat Sertoli cells. Molecular and Cellular Endocrinology 1(49): 51-62.

Perry, L.M. 1980. Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses. Cambridge: The MIT Press. p. 94.

Pusparanee, H., Halimah A.S. & Mahanem, M.N. 2008. Effects of Gynura procumbens extract and glibenclamide on sperm quality and specific activity of testicular lactate dehydrogenase in streptozotocin-induced diabetic rats. Malaysian Journal of Biochemistry and Molecular Biology 16(2): 10-14.

Butchi Akondi, R., Kumar, P., Annapurna, A. & Pujari, M. 2011. Protective effect of rutin and naringin on sperm quality in streptozotocin (STZ) induced type I diabetic rats. Iranian Journal of Pharmaceutical Research 10(3): 585-596.

Rosidah, Mun, F.Y., Amirin, S., Mariam, A., Gabriel, A.A. & Mohd Zaini Asmawi. 2009. Toxicology evaluation of standardized methanol extract of Gynura procumbens. Journal of Ethnopharmacology 123: 244-249.

Shrilata, B. & Muralidhara. 2007. Early oxidative stress in testes and epidydimal sperm in streptozotocin-induced diabetic mice: Its progression and genotoxic consequences. J. Reproductive Toxicology 23(4): 578-587.

Tartarin, P., Moison, D., Guilbert, E., Dupont, J., Hebert, R., Rouiller-Fabre, V., Frydman, N., Pozzi, S., Frydman, R., Lecureuil, C. & Froment, P. 2012. Metformin exposure effects human and mouse fetal testicular cells. Human Reproduction 27(11): 3304-3314.

Turk, G., Sonmez, M., Aydin, M., Yuce, A. & Aksoy, H. 2008. Effects of promegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clinical Nutrition 27: 289-296.

William, G. & Pickup, J. 2004. Handbook of Diabetes. Massachusettes: Blakewell Publishing.

World Health Organisation. 2010. Laboratory Manual for the Examination and Processing of Human Semen. Geneva: WHO Press.

Zurina Hassan, Mun Fei Yam, Mariam Ahmad & Ahmad Pauzi M. Yusof. 2010. Antidiabteic properties and mechanisms of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules 15: 9008-9023.

 

 

*Pengarang untuk surat-menyurat; email: mahanem@ukm.edu.my

 

 

 

 

 

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