Sains Malaysiana 48(1)(2019): 69–73

http://dx.doi.org/10.17576/jsm-2019-4801-08

Short-Term Cytotoxicity of Zinc Oxide Nanoparticles on Chlorella vulgaris

(Ketoksikan Jangka Pendek Zarah Nano Zink Oksida ke atas Chlorella vulgaris)

SINOUVASSANE DJEARAMANE¹, LING SHING WONG², YANG MOOI LIM³ & POH FOONG LEE^1*

¹Department of Mechatronics & Biomedical Engineering, Lee Kong Chian Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 43000 Bandar Sungai Long, Selangor Darul Ehsan, Malaysia

²Faculty of Health and Life Sciences, INTI International University, 71800 Nilai, Negeri Sembilan, Malaysia

³Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, 43000 Bandar Sungai Long, Selangor Darul Ehsan, Malaysia

Diserahkan: 26 April 2017/Diterima: 12 Februari 2018

ABSTRACT

Zinc oxide nanoparticles (ZnO NPs) are widely used in industrial and personal care products. The use of these nanoparticles (NPs) has created residues that contaminate the environment, thus cytotoxicity studies of the NPs in biological system is required. Most of the recent cytotoxicity studies has however focused on long-term exposure of the NPs to the biological system. In this study, the cytotoxicity effects of short-term exposure of ZnO NPs to Chlorella vulgaris are reported. The algal cells were exposed to 10, 50, 100, 150, and 200 mg/L of ZnO NPs for 12 h. The toxicity effects of ZnO NPs were then determined through the changes in fluorescence emission of chlorophyll, algal biomass and the viable cell count. The results showed a decrease in the chlorophyll content, algal biomass and cell viability after treatment with ZnO NPs as compared with control. Through this study, the effects of ZnO NPs to C. vulgaris were confirmed. The significant responses of the algal cells to ZnO NPs in a short duration of exposure reflect the potential of the algal cells to be used as bioindicators of ZnO NPs in the aquatic environment.

Keywords: Algal cells; Chlorella vulgaris; short-term cytotoxicity; zinc oxide nanoparticles

ABSTRACT

Zarah nano zink oksida (ZnO NP) digunakan secara meluas dalam produk industri dan penjagaan peribadi. Penggunaan zarah nano tersebut telah menghasilkan residu yang membawa pencemaran kepada alam sekitar. Oleh itu, kajian kesan kesitotoksikan ZnO NP terhadap sistem biologi amat diperlukan. Namun, kajian kesan kesitotoksikan pada masa ini lebih tertumpu kepada kesan jangka panjang terhadap sistem biologi. Oleh itu, dalam kajian ini, kesan kesitotoksikan jangka masa pendek ZnO NP terhadap Chlorella vulgaris telah dilaporkan. Sel alga tersebut telah didedahkan kepada ZnO NP dengan kepekatan yang berbeza (10, 50, 100, 150 dan 200 mg/L) untuk jangka masa sehingga 12 jam. Kesan kesitotoksikan ZnO NP pada sel alga telah dipantau melalui perubahan pendarfluoran klorofil, perubahan biojisim dan kemandirian sel alga. Pendedahan sel alga kepada ZnO NP telah mengakibatkan pengurangan kandungan klorofil, biojisim dan kemandirian sel alga. Melalui kajian ini, kesan ZnO NP terhadap C. vulgaris telah dikenal pasti. Keupayaan C. vulgaris bertindak balas dengan ZnO NP dalam jangka masa yang pendek menampilkan potensi alga tersebut digunakan sebagai petunjuk biologi untuk penilaian ketoksikan ZnO NP dalam persekitaran akuatik.

Kata kunci: Chlorella vulgaris; kesitotoksikan jangka pendek; sel alga; zarah nano zink oksida

RUJUKAN

Adams, L., Lyon, D., McIntosh, A. & Alvarez, P. 2006. Comparative toxicity of nano-scale TiO2, SiO2 and ZnO water suspensions. Water Science and Technology 54(11- 12): 327-334.

Aruoja, V., Dubourguier, H.C., Kasemets, K. & Kahru, A. 2009. Toxicity of nanoparticles of CuO, ZnO and TiO 2 to microalgae Pseudokirchneriella subcapitata. Science of the Total Environment 407(4): 1461-1468.

Barhoumi, L. & Dewez, D. 2013. Toxicity of superparamagnetic iron oxide nanoparticles on green alga Chlorella vulgaris. BioMed Research International 2013: 647974.

Bhuvaneshwari, M., Iswarya, V., Archanaa, S., Madhu, G.M., Suraish Kumar, G.K., Nagarajan, R., Chandrasekaran, N. & Mukherjee, A. 2015. Cytotoxicity of ZnO NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C, visible and dark conditions. Aquatic Toxicology 162: 29-38.

Brayner, R., Dahoumane, S.A., Yéprémian, C., Djediat, C., Meyer, M., Couté, A. & Fiévet, F. 2010. ZnO nanoparticles: synthesis, characterization, and ecotoxicological studies. Langmuir 26(9): 6522-6528.

Chen, P., Powell, B.A., Mortimer, M. & Ke, P.C. 2012. Adaptive interactions between zinc oxide nanoparticles and Chlorella sp. Environmental Science & Technology 46(21): 12178- 12185.

Dastjerdi, R. & Montazer, M. 2010. A review on the application of inorganic nano-structured materials in the modification of textiles: Focus on anti-microbial properties. Colloids and Surfaces B: Biointerfaces 79(1): 5-18.

Gong, N., Shao, K., Feng, W., Lin, Z., Liang, C. & Sun, Y. 2011. Biotoxicity of nickel oxide nanoparticles and bio-remediation by microalgae Chlorella vulgaris. Chemosphere 83(4): 510-516.

Hazeem, L.J., Bououdina, M., Rashdan, S., Brunet, L., Slomianny, C. & Boukherroub, R. 2016. Cumulative effect of zinc oxide and titanium oxide nanoparticles on growth and chlorophyll a content of Picochlorum sp. Environmental Science and Pollution Research 23(3): 2821-2830.

Iswarya, V., Bhuvaneshwari, M., Alex, S.A., Iyer, S., Chaudhuri, G., Chandrasekaran, P.T., Bhalerao, G.M., Chakravarty, S, Raichur, A.M., Chandrasekaran, N. & Mukherjee, A. 2015. Combined toxicity of two crystalline phases (anatase and rutile) of Titania nanoparticles towards freshwater microalgae: Chlorella sp. Aquatic Toxicology 161: 154-169.

Ji, J., Long, Z. & Lin, D. 2011. Toxicity of oxide nanoparticles to the green algae Chlorella sp. Chemical Engineering Journal 170(2): 525-530.

Li, X., Zhou, S. & Fan, W. 2016. Effect of nano-Al2O3 on the toxicity and oxidative stress of copper towards Scenedesmus obliquus. International Journal of Environmental Research and Public Health 13(6): 575.

Miao, A.J., Schwehr, K.A., Xu, C., Zhang, S.J., Luo, Z., Quigg, A. & Santschi, P.H. 2009. The algal toxicity of silver engineered nanoparticles and detoxification by exopolymeric substances. Environmental Pollution 157(11): 3034-3041.

Moore, M. 2006. Do nanoparticles present ecotoxicological risks for the health of the aquatic environment? Environment International 32(8): 967-976.

Osmond, M.J. & Mccall, M.J. 2010. Zinc oxide nanoparticles in modern sunscreens: An analysis of potential exposure and hazard. Nanotoxicology 4(1): 15-41.

Popov, A., Priezzhev, A., Lademann, J. & Myllylä, R. 2005. TiO2 nanoparticles as an effective UV-B radiation skin-protective compound in sunscreens. Journal of Physics D: Applied Physics 38(15): 2564.

Sadiq, I.M., Pakrashi, S., Chandrasekaran, N. & Mukherjee, A. 2011. Studies on toxicity of aluminum oxide (Al2O3) nanoparticles to microalgae species: Scenedesmus sp. and Chlorella sp. Journal of Nanoparticle Research 13(8): 3287-3299.

Shing, W.L., Heng, L.Y. & Surif, S. 2012. The fluorometric response of cyanobateria to short exposure of heavy metal. Advances in Environmental Biology 6(1): 103-108.

Song, W., Zhang, J., Guo, J., Zhang, J., Ding, F., Li, L. & Sun, Z. 2010. Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicology Letters 199(3): 389-397.

Suman, T., Rajasree, S.R. & Kirubagaran, R. 2015. Evaluation of zinc oxide nanoparticles toxicity on marine algae Chlorella vulgaris through flow cytometric, cytotoxicity and oxidative stress analysis. Ecotoxicology and Environmental Safety 113: 23-30.

Tang, Y., Li, S., Qiao, J., Wang, H. & Li, L. 2013. Synergistic effects of nano-sized titanium dioxide and zinc on the photosynthetic capacity and survival of Anabaena sp. International Journal of Molecular Sciences 14(7): 14395- 14407.

Wiesner, M.R., Lowry, G.V., Alvarez, P., Dionysiou, D. & Biswas, P. 2006. Assessing the risks of manufactured nanomaterials. Environmental Science & Technology 40(14): 4336-4345.

Xiaoxiao, C., Xing, Z., Rui, L., Hanchao, Y., Zhisong, L. & Xu, Y. 2012. Photosynthetic toxicity and oxidative damage induced by nano-Fe3O4 on Chlorella vulgaris in aquatic environment. Open Journal of Ecology 2(1): 21-28.

Zhao, J. & Castranova, V. 2011. Toxicology of nanomaterials used in nanomedicine. Journal of Toxicology and Environmental Health, Part B 14(8): 593-632.

Zhou, H., Wang, X., Zhou, Y., Yao, H. & Ahmad, F. 2014. Evaluation of the toxicity of ZnO nanoparticles to Chlorella vulgaris by use of the chiral perturbation approach. Analytical and Bioanalytical Chemistry 406(15): 3689-3695.

*Pengarang untuk surat-menyurat; email: leepf@utar.edu.my