Sains Malaysiana 51(9)(2022): 2775-2788

http://doi.org/10.17576/jsm-2022-5109-04

 

Variability of White Spot Syndrome Virus (WSSV) Envelope Protein VP28 from Diseased Shrimp (Litopenaeus vannamei) in Indonesia

(KepelbagaianVirus Sindrom Bintik Putih (WSSV) Protein Sampul VP28 daripada Udang (Litopenaeus vannamei) Berpenyakit di Indonesia)

 

A.C.M.A.R. TASAKKA1,*, G. LATAMA1, A.A. HIDAYANI1, A. PARENRENGI2, A. TENRIULO2, A.N.R. RELATAMI3, A.M. MOORE4 & A. SHAHEEN5

 

1Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, South Sulawesi, Indonesia

2Research Center for Fishery, National Research and Innovation Agency, Indonesia

3Veterinary Medicine Department, Faculty of Medicine, Hasanuddin University, Makassar,

South Sulawesi, Indonesia

4Graduate School, Hasanuddin University, Makassar, South Sulawesi, Indonesia

5National Institute of Oceanography, Karachi, Pakistan

 

Diserahkan: 13 Disember 2021/Diterima: 25 April 2022

 

Abstract

White Spot Disease (WSD) is a viral disease affecting crustaceans. Caused by the White Spot Syndrome Virus (WSSV), this disease has caused significant mortality in commercially cultivated marine shrimp species with severe impacts on the shrimp farming industry and may be a threat to wild shrimp stocks. Thorough studies on the molecular biology of this pathogen are urgently needed to improve understanding of the virus at a molecular level, including variation in key viral protein (VP) components of the WSSV virion. This study aimed to isolate and characterize WSSV VP28 gene encoding envelope proteins from Indonesian Pacific white shrimp (Litopenaeus vannamei) isolates. Infected juvenile shrimp were collected from Pangkep, Barru, and Pinrang Districts in South Sulawesi, Indonesia. Genomic DNA was isolated from infected shrimp muscle tissue using a DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl trimethyl ammonium bromide) DNA extraction procedure. The WSSV VP28 DNA sequences from Pangkep, Barru, and Pinrang isolates were 640-680 bp in length. Homology of Pangkep isolates with isolates from Barru and Pinrang was 97-99%. BLAST-N (Basic Local Alignment Search Tool-Nucleotide) analysis showed isolates from all three sites clustered with WSSV VP28 accessions from China, Indonesia, Japan, South Carolina and Vietnam. These results increase the geographic spread and host taxon coverage of WSSV VP28 sequence data for Indonesia.

 

Keywords: Litopenaeus vannamei; South Sulawesi; VP28; viral protein variability; WSSV

 

Abstrak

Penyakit Bintik Putih (WSD) adalah penyakit virus yang menjejaskan krustasea. Disebabkan oleh Virus Sindrom Bintik Putih (WSSV), penyakit ini telah menyebabkan kematian yang ketara dalam spesies udang marin yang diusahakan secara komersial dengan kesan yang teruk terhadap industri penternakan udang dan mungkin menjadi ancaman kepada stok udang liar. Kajian menyeluruh tentang biologi molekul patogen ini amat diperlukan untuk meningkatkan pemahaman virus pada tahap molekul, termasuk variasi dalam komponen protein virus (VP) utama virion WSSV. Kajian ini bertujuan untuk mengasingkan dan mencirikan gen WSSV VP28 pengekodan protein sampul surat daripada pencilan udang putih Pasifik Indonesia (Litopenaeus vannamei). Udang juvana yang dijangkiti dikumpul dari Daerah Pangkep, Barru dan Pinrang di Sulawesi Selatan, Indonesia. DNA genom telah diasingkan daripada tisu otot udang yang dijangkiti menggunakan prosedur pengekstrakan DNA DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl trimethyl ammonium bromide). Urutan DNA WSSV VP28 daripada pencilan Pangkep, Barru dan Pinrang adalah 640-680 bp panjang. Homologi pencilan Pangkep dengan pencilan Barru dan Pinrang adalah 97-99%. Analisis BLAST-N (Alat Carian Penjajaran Tempatan Asas-Nukleotida) menunjukkan pencilan daripada ketiga-tiga tapak berkelompok dengan aksesi WSSV VP28 dari China, Indonesia, Jepun, Carolina Selatan dan Vietnam. Hasil ini meningkatkan sebaran geografi dan liputan takson hos bagi data jujukan WSSV VP28 untuk Indonesia.

 

Kata kunci: Kebolehubahan protein virus; Litopenaeus vannamei; Sulawesi Selatan; VP28; WSSV

 

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