Sains Malaysiana 47(11)(2018): 2731–2739

http://dx.doi.org/10.17576/jsm-2018-4711-16

 

The Effect of Palm Tocotrienol on Surface Osteoblast and Osteoclast in Excess Glucocorticoid Osteoporotic Rat Model

(Kesan Tokotrienol Sawit kepada Permukaan Osteoblas dan Osteoklas dalam Model Tikus Berlebihan Osteoporosis Glukokortikoid)

MOHAMAD FAIRUZ YAHAYA1*, AZIZI ZAINODIN1, REINUKAA PUPATHY1, EDWIN ONG HOCK MIN1, NURUL HUSNA ABU BAKAR1, NOR AZIMAH ZAMRI1, HALIM ISMAIL2 & ELVY SUHANA MOHD RAMLI1

 

1Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

2Department of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

 

Diserahkan: 20 Mac 2018/Diterima: 5 Julai 2018

 

ABSTRACT

A balanced interaction between osteoblast and osteoclast plays a major role in maintaining bone strength and structural integrity. Tocotrienol, a palm derivative with potent antioxidant properties showed an anti-osteoporotic effect but little is known about its mechanism of action. Hence, this research was conducted to determine the effects of palm tocotrienol on the surface osteoblast and osteoclast of the the glucocorticoid-induced osteoporotic bones. The study involved 40 male Sprague-Dawley rats weighing 250-300 g which were randomly divided into an equal number of Baseline, Sham, Adrx+Dexa (adrenalectomized with dexamethasone), Adrx+Dexa+ATF (adrenalectomized with dexamethasone supplemented with α-tocopherol) and Adrx+Dexa+PTT (adrenalectomized with dexamethasone supplemented with palm tocotrienol). Bloods were taken prior to sacrifice for serum biomarkers and either tibia or femur was harvested for bone biomarkers, gene expressions analysis and histological studies. A double-blinded experiment was performed to calculate the number of total cells (osteoblasts and osteoclast) with intact nuclei within five fields of view. While serum osteocalcin and gene expression did not show any changes, CTX level was increased in the Adrx+Dexa group but reduced after the supplementation of palm tocotrienol. Supplementation of palm tocotrienol also significantly decrease the osteoclast population (p<0.05) compared to Adrx+Dexa group. In contrast, osteoblast population showed no significant difference across the groups. The result showed that palm tocotrienol acts by modulating the osteoclasts but not osteoblast, which revert the dynamics of bone cells population in the osteoporotic bone to its pre-osteoporotic levels. Supplements of tocotrienol in daily diet may be beneficial in preventing osteoporosis.

 

Keywords: Antioxidant; osteoblast; osteoclast; osteoporosis; vitamin E

 

 

ABSTRAK

Interaksi seimbang antara osteoblas dan osteoklas memainkan peranan utama dalam mengekalkan kekuatan dan integriti struktur tulang. Tokotrienol, terbitan sawit dengan sifat antioksidan yang kuat telah menunjukkan kesan anti-osteoporosis tetapi amat sedikit diketahui tentang mekanisme tindakannya. Oleh yang demikian, kajian ini dijalankan untuk menentukan kesan tokotrienol sawit ke atas sel permukaan osteoblas dan osteoklas pada tulang osteoporosis aruhan glukokortikoid. Kajian ini melibatkan 40 ekor tikus Sprague-Dawley jantan dengan berat antara 250-300 g yang dibahagikan secara rawak kepada kumpulan Kawalan, Sham, Adrx+Dexa (adrenalektomi dengan deksamethason), Adrx+Dexa+ATF (adrenalektomi dengan deksamethason ditambah α-tokoferol) dan Adrx+Dexa+PTT (adrenalektomi dengan deksamethason ditambah tocotrienol sawit) secara oral selama dua bulan. Darah diambil daripada tikus sebelum dikorbankan dan tulang tibia atau femur kemudiannya diambil untuk kajian petanda biokimia dan histologi. Kajian ‘double blinded’ dilakukan dengan menghitung jumlah keseluruhan sel (osteoblas dan osteoklas) dengan nukleus dalam lima medan pandangan. Sementara serum osteokalsin dan ekspresi gen tidak menunjukkan sebarang perubahan, aras CTX meningkat pada kumpulan Adrx+Dexa tetapi menurun dengan suplementasi tocotrienol sawit. Suplementasi tocotrienol sawit juga menyebabkan penurunan ketara bilangan osteoklas (p<0.05) berbanding kumpulan Adrx+Dexa. Sebaliknya, populasi osteoblas tidak menunjukkan perbezaan yang signifikan pada kesemua kumpulan. Keputusan kajian menunjukkan bahawa tokotrienol sawit bertindak dengan memodulasi osteoklas tetapi bukan osteoblas yang mengembalikan dinamik osteoblas dan populasi osteoblas dalam tulang osteoporosis ke tahap pra-osteoporosis. Tambahan tokotrienol dalam diet harian mampu memberi manfaat dalam mencegah osteoporosis.

 

Kata kunci: Antioksidan; osteoblas; osteoklas; osteoporosis; vitamin E

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*Pengarang untuk surat-menyurat; email: mfairuzy@ukm.edu.my

 

 

 

 

 

 

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