• Food Science
• Molecular Biology
• Advance Material Science
• Chemical Industry
Brand : Bruker
Model : Avance III 600MHz Equipped with Cryoprobe and Triple Resonance Channel
Fourier Transform Nuclear Magnetic Resonance (FT-NMR) is an analytical technique used to analyse the structure of molecules range from a small organic molecule or metabolite, to a mid-sized peptide or a natural/synthetic product, all the way up to proteins of several tens of kDa in molecular weight.
Attached cryoprobe offers high sensitivity which allows measurement of minute amount of sample while triple resonance channel facilitate the NMR structure analysis of biological macromolecules.
NMR works based on magnetic properties which are called “Spin” in active nuclei such as Hydrogen and Carbon. A typical compound might consist of carbon, hydrogen and oxygen atoms.
In its simplest form, an NMR experiment consists of three steps:
1. Place the sample in a static magnetic field.
2. Excite nuclei in the sample with a radio frequency pulse.
3. Measure the frequency of the signals emitted by the sample.
4. From the emitted frequencies, the information about the bonding and arrangement of the atoms in the sample is recorded as Free Induction Decay or FID. By using mathematical calculation, Fourier Transform, the FID data is converted to spectra.
MOHD ZAHID MD YUSOFF