High Performance Liquid Chromatography (HPLC)

Brand : Waters
Model : 2695 and 2795 with Photo-Diode-Array Detector

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

The sample mixture to be separated and analysed is introduced in a discrete small volume (typically microliters) into the stream of mobile phase percolating through the column. The components of the sample move through the column at different velocities, which are a function of specific physical interactions with the adsorbent (also called stationary phase). The velocity of each component depends on its chemical nature, the nature of the stationary phase (column) and the composition of the mobile phase. The time at which a specific analyte elutes (emerges from the column) is called its retention time. The retention time measured under particular conditions is an identifying characteristic of a given analyte.


High-performance liquid chromatography (HPLC) is one of the most powerful tools in analytical chemistry. It has become a very common laboratory instrument that in fact it is the third most common instrument found in laboratories, trailing behind the balance and pH meter. HPLC has the ability to separate, identify, and quantitate compounds over a wide range of polarity that are present in any sample that can be dissolved in a liquid. Today, compounds in trace concentrations as low as parts per trillion (ppt) may easily be identified with the appropriate detector.

HPLC can be applied to just about any sample, such as pharmaceuticals, environmental, forensic, food, nutraceuticals, and industrial chemicals.
• Environmental monitoring
• Food, beverage, flavour and fragrance analysis
• Forensic and criminal cases
• Biological and pesticides detections
• Astro chemistry and geo chemical research
• Medicine and pharmaceutical applications
• Petrochemical and hydrocarbons analysis
• Clinical toxicology
• Academic research
• Industrial applications
Sample Requirements
• Liquid - organic solvents (MeOH, ACN)
• High purity water
• Chloroform

Additional information


Diploma (UiTM) Science



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