UKM Medical Molecular Biology Institute

 Leading Institute in Molecular Medicine

CRISPR Genome Editing Workshop 2022

  •  

    Facilitators

    Dr Saiful Effendi Syafruddin

    Research Fellow

    Cancer Biology, Immunology & Functional Genomics

    effendisy@ppukm.ukm.edu.my

    +603-91459072

    BSc. (Hons) Biotechnology (RIT, USA), MSc. Molecular Genetics (Uni of Pennsylvania, USA), PhD Medical Sciences (University of Cambridge)

     

     

     

    Dr. Mohamad Aimanuddin Mohtar

    Research Fellow

    Cancer Biology and Functional Proteomics

    m.aimanmohtar@ppukm.ukm.edu.my

    +603-91459062

    BSc. Biotechnology (H. Hons) (USA), MSc. Molecular Biology (Uni of Pennsylvania, USA), PhD Molecular & Clinical Medicine (Uni of Edinburgh, UK)

     

     

     

    Sponsors

     

     

  • Introduction

    The UMBI 2022 CRISPR workshop is tailored to give an overview of the Nobel-prize winning genome editing technology combined with several hands-on laboratory practical. This workshop consists of several lecture sessions that will comprehensively cover the important background and principal aspects of CRISPR technology. These include CRISPR initial discovery as the mechanism of bacteria adaptive immune response, CRISPR repurposing for eukaryotic gene targeting, CRISPR wide range of applications in the field of molecular biology and life sciences, and CRISPR genome editing experimental workflow. The participants will also get hands-on experience as well as tips and tricks from the experts focusing on the key laboratory techniques within this technology.

    When?

    18-20th October 2022
    8.30am – 4.30pm

    Where?

    UKM Medical Molecular Biology Institute (UMBI), Jalan Yaacob Latiff, 56000 Kuala Lumpur

    Workshop fees

    RM 950 (Non-Student – Scientist, researcher, specialist, academicians, industry partners etc)

    RM 800 (Student)

    Fees include workshop materials and meals (3 times a day)

    Why should you join?

    • Learn how to perform CRISPR genome editing experiment using the cost-effective conventional approach without completely relying on the commercial kits.
    • Facilitated by researchers who have extensive CRISPR genome editing with successful results and high-impact publications.
    • Opportunity to perform hands-on laboratory practical; choosing vector system, sgRNA designing and cloning, and delivering into cells. Important tips and troubleshooting strategies for a successful CRISPR experiment will also be shared. 
    • Free complete in-house CRISPR guidebook written by the facilitators

    Who should attend?

    We welcome all Researchers, Scientists, Postgraduate Students, Application Specialists, or anyone who is interested in learning this CRISPR technology to attend. Although it is not essential, having basic molecular biology knowledge would be an advantage as the course will cover few lectures and practical sessions that are in intermediate-to-advanced levels. Nonetheless, we will accommodate all participants who are keen to join the workshop and learn this CRISPR genome editing technology.

    The workshop is limited to only 20 seats

    Past workshop

    We had a successful inaugural CRISPR genome editing workshop that was held on the 18-20th November 2019 (Link: https://www.ukm.my/umbi/news/crispr-gene-editing-workshop-2019/). The workshop was attended by researchers and students from local and overseas institutions and has garnered several collaborations with participants who mainly come from the academia. In addition to the active collaboration with local and overseas researchers for CRISPR works, the facilitators have also been invited to give talks in several webinars and pre-conference workshops about this CRISPR genome editing technology.

  • UMBI CRISPR Workshop (18-20 October 2022)

     

    Tuesday 18 October 2022 – Day 1

     

     

    TIME PROGRAMME
    0800-0900 REGISTRATION AND BREAKFAST
     

     

     

    0900

     

     Lecture 1 : Introduction and principle of CRISPR/Cas9 technology

     

    ·         Brief history

    ·         Genome editing tools

    ·         Principle of CRISPR-based genome editing

    1015 SHORT BREAK
     

     

     

    1030

     

    Lecture 2: Cas9 variants and applications of CRISPR/Cas9 technology

     

    ·         Cas9 Nickase & Dead Cas9

    ·         Base Editing & Prime Editing

    ·         Research and Clinical Applications

    ·         Ethical Issues

     

    1145 SHORT BREAK
     

     

     

    1200

     

    Lecture 3: Starting your CRISPR experiment

     

    ·         Developing Relevant Biological Questions

    ·         General Gene Knockout and Knock-In Experimental Designs

    ·         Choosing the Right CRISPR System

    1300-1415 LUNCH BREAK
    1415  Practical 1 – sgRNA design

     

    o    Extracting genomic information (Ensembl Demo)

    o    sgRNAs designing (CRISPick Demo)

    o    sgRNAs target region visualization (Snapgene Viewer Demo)

    o    Primer design to amplify targeted region

     

    1630 TEA & END OF DAY 1

     

    Wednesday 19 October 2022 – Day 2

     
    <t

    TIME PROGRAMME
    0800-0900 BREAKFAST
     

     

     

    0900

     

     Lecture 4 : CRISPR-Cas9 experimental workflow

     

    ·         Knockout/Knockin workflow

    ·         sgRNA cloning strategy

    1030 SHORT BREAK
     

     

     

    1045

     

    Practical 2

     

    o    Expression vector digestion

    o    sgRNAs annealing

    o    sgRNAs ligation

    o    Bacteria transformation

     

    1300-1415 LUNCH BREAK
     

     

     

    1415

     

    Lecture 5: Cells delivery and selection of positive cells

     

    ·         CRISPR components delivery into the cells

    ·         Determination of delivery efficiency

    ·         Antibiotic selection (pooled cells isolation)

    ·         Single cell sorting and dilution

     

    1515-1530 SHORT BREAK
    1530  

    Practical 3

     

    o    CRISPR plasmids transfection

    o    T7E1 endonuclease assay (PCR product rehybridization)

     

    1630 TEA & END OF DAY 2

     

    Thursday 20 October 2022 – Day 3

     

    TIME PROGRAMME
    0800-0900 BREAKFAST
     

     

    0900

     

     Practical 4

     

    o    Transfected cells visualization

    o    T7E1 endonuclease assay (Digestion)

     

    1015-1030 SHORT BREAK
     

     

     

    1030

     

     Sponsored Talk : PLT Scientific

     

     Multiplex Western Blot Imaging and More with Azure Gel Imaging System

     

    1130-1145 SHORT BREAK
     

     

    1145

     

    Practical 5

     

    o    T7E1 endonuclease assay (Result discussion)

    o    Azure Biosystem Demo (Western blot Imaging)

     

    1300-1415 LUNCH BREAK
    1415  

    Lecture 6: Characterization of edited cells and CRISPR-Cas9 case studies

     

    Networking and Discussion

     

    1630 TEA & END OF WORKSHOP

     

    Link in PDF : UMBI CRISPR Workshop 2022 Tentative

     

  • The CRISPR gene editing workshop will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.

    For more information about the venue, please visit the UMBI website at https://www.ukm.my/umbi/.

    Please take note that the accommodation is NOT provided. Kindly refer to the Accommodation tab for further information.

    Getting here:

    • Public transport: 

    The nearest MRT station is Taman Pertama/Taman Midah
    The nearest LRT stations is Salak Selatan/Bandar Tun Razak
    Then take a GRAB or taxi to UMBI.

    • By Car:

    Participant can park at Zone L parking in the UKM Medical Center (see attached map)

     

     

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html

    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292

  • Hotels nearby UMBI:

    • Hotel Sri Permaisuri Cheras (3-star hotel)
    • Hotel EST Kuala Lumpur
    • International Youth Centre (IYC) Residence
    • Hotel Maple Inn
    • V Hotel Kuala Lumpur
    • Crystal Garden Hotel

    Low Budget hotels are also available nearby the UMBI. Please refer to any hotel booking website for further information.

    **Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

  • Contact details:

    Miss Siti Nur Hasanah: +603 9145 9087 / 9185  (email: hasanahyusuf@ukm.edu.my)
    UKM Medical Molecular Biology Institute (UMBI),
    Jalan Yaacob Latiff, Bandar Tun Razak,
    56000 Cheras, Kuala Lumpur, Malaysia