CRISPR Genome Editing Workshop 2022
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Facilitators
Dr Saiful Effendi Syafruddin
Research Fellow
Cancer Biology, Immunology & Functional Genomics
effendisy@ppukm.ukm.edu.my
+603-91459072
BSc. (Hons) Biotechnology (RIT, USA), MSc. Molecular Genetics (Uni of Pennsylvania, USA), PhD Medical Sciences (University of Cambridge)
Dr. Mohamad Aimanuddin Mohtar
Research Fellow
Cancer Biology and Functional Proteomics
m.aimanmohtar@ppukm.ukm.edu.my
+603-91459062
BSc. Biotechnology (H. Hons) (USA), MSc. Molecular Biology (Uni of Pennsylvania, USA), PhD Molecular & Clinical Medicine (Uni of Edinburgh, UK)
Sponsors
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Introduction
The UMBI 2022 CRISPR workshop is tailored to give an overview of the Nobel-prize winning genome editing technology combined with several hands-on laboratory practical. This workshop consists of several lecture sessions that will comprehensively cover the important background and principal aspects of CRISPR technology. These include CRISPR initial discovery as the mechanism of bacteria adaptive immune response, CRISPR repurposing for eukaryotic gene targeting, CRISPR wide range of applications in the field of molecular biology and life sciences, and CRISPR genome editing experimental workflow. The participants will also get hands-on experience as well as tips and tricks from the experts focusing on the key laboratory techniques within this technology.
When?
18-20th October 2022
8.30am – 4.30pmWhere?
UKM Medical Molecular Biology Institute (UMBI), Jalan Yaacob Latiff, 56000 Kuala Lumpur
Workshop fees
RM 950 (Non-Student – Scientist, researcher, specialist, academicians, industry partners etc)
RM 800 (Student)
Fees include workshop materials and meals (3 times a day)
Why should you join?
- Learn how to perform CRISPR genome editing experiment using the cost-effective conventional approach without completely relying on the commercial kits.
- Facilitated by researchers who have extensive CRISPR genome editing with successful results and high-impact publications.
- Opportunity to perform hands-on laboratory practical; choosing vector system, sgRNA designing and cloning, and delivering into cells. Important tips and troubleshooting strategies for a successful CRISPR experiment will also be shared.
- Free complete in-house CRISPR guidebook written by the facilitators
Who should attend?
We welcome all Researchers, Scientists, Postgraduate Students, Application Specialists, or anyone who is interested in learning this CRISPR technology to attend. Although it is not essential, having basic molecular biology knowledge would be an advantage as the course will cover few lectures and practical sessions that are in intermediate-to-advanced levels. Nonetheless, we will accommodate all participants who are keen to join the workshop and learn this CRISPR genome editing technology.
The workshop is limited to only 20 seats
Past workshop
We had a successful inaugural CRISPR genome editing workshop that was held on the 18-20th November 2019 (Link: https://www.ukm.my/umbi/news/crispr-gene-editing-workshop-2019/). The workshop was attended by researchers and students from local and overseas institutions and has garnered several collaborations with participants who mainly come from the academia. In addition to the active collaboration with local and overseas researchers for CRISPR works, the facilitators have also been invited to give talks in several webinars and pre-conference workshops about this CRISPR genome editing technology.
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UMBI CRISPR Workshop (18-20 October 2022)
Tuesday 18 October 2022 – Day 1
TIME PROGRAMME 0800-0900 REGISTRATION AND BREAKFAST 0900
Lecture 1 : Introduction and principle of CRISPR/Cas9 technology
· Brief history
· Genome editing tools
· Principle of CRISPR-based genome editing
1015 SHORT BREAK 1030
Lecture 2: Cas9 variants and applications of CRISPR/Cas9 technology
· Cas9 Nickase & Dead Cas9
· Base Editing & Prime Editing
· Research and Clinical Applications
· Ethical Issues
1145 SHORT BREAK 1200
Lecture 3: Starting your CRISPR experiment
· Developing Relevant Biological Questions
· General Gene Knockout and Knock-In Experimental Designs
· Choosing the Right CRISPR System
1300-1415 LUNCH BREAK 1415 Practical 1 – sgRNA design o Extracting genomic information (Ensembl Demo)
o sgRNAs designing (CRISPick Demo)
o sgRNAs target region visualization (Snapgene Viewer Demo)
o Primer design to amplify targeted region
1630 TEA & END OF DAY 1 Wednesday 19 October 2022 – Day 2
<tTIME PROGRAMME 0800-0900 BREAKFAST 0900
Lecture 4 : CRISPR-Cas9 experimental workflow
· Knockout/Knockin workflow
· sgRNA cloning strategy
1030 SHORT BREAK 1045
Practical 2
o Expression vector digestion
o sgRNAs annealing
o sgRNAs ligation
o Bacteria transformation
1300-1415 LUNCH BREAK 1415
Lecture 5: Cells delivery and selection of positive cells
· CRISPR components delivery into the cells
· Determination of delivery efficiency
· Antibiotic selection (pooled cells isolation)
· Single cell sorting and dilution
1515-1530 SHORT BREAK 1530 Practical 3
o CRISPR plasmids transfection
o T7E1 endonuclease assay (PCR product rehybridization)
1630 TEA & END OF DAY 2 Thursday 20 October 2022 – Day 3
TIME PROGRAMME 0800-0900 BREAKFAST 0900
Practical 4
o Transfected cells visualization
o T7E1 endonuclease assay (Digestion)
1015-1030 SHORT BREAK 1030
Sponsored Talk : PLT Scientific
Multiplex Western Blot Imaging and More with Azure Gel Imaging System
1130-1145 SHORT BREAK 1145
Practical 5
o T7E1 endonuclease assay (Result discussion)
o Azure Biosystem Demo (Western blot Imaging)
1300-1415 LUNCH BREAK 1415 Lecture 6: Characterization of edited cells and CRISPR-Cas9 case studies
Networking and Discussion
1630 TEA & END OF WORKSHOP Link in PDF : UMBI CRISPR Workshop 2022 Tentative
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The CRISPR gene editing workshop will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.
For more information about the venue, please visit the UMBI website at https://www.ukm.my/umbi/.
Please take note that the accommodation is NOT provided. Kindly refer to the Accommodation tab for further information.
Getting here:
- Public transport:
The nearest MRT station is Taman Pertama/Taman Midah
The nearest LRT stations is Salak Selatan/Bandar Tun Razak
Then take a GRAB or taxi to UMBI.- By Car:
Participant can park at Zone L parking in the UKM Medical Center (see attached map)
WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html
GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292
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Hotels nearby UMBI:
- Hotel Sri Permaisuri Cheras (3-star hotel)
- Hotel EST Kuala Lumpur
- International Youth Centre (IYC) Residence
- Hotel Maple Inn
- V Hotel Kuala Lumpur
- Crystal Garden Hotel
Low Budget hotels are also available nearby the UMBI. Please refer to any hotel booking website for further information.
**Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.
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Contact details:
Miss Siti Nur Hasanah: +603 9145 9087 / 9185 (email: hasanahyusuf@ukm.edu.my)
UKM Medical Molecular Biology Institute (UMBI),
Jalan Yaacob Latiff, Bandar Tun Razak,
56000 Cheras, Kuala Lumpur, Malaysia