Real Time PCR Workshop: Real Time PCR for Gene Expression Analysis

[countdown event=”Countdown: Real Time PCR Workshop: Real Time PCR for Gene Expression Analysis” date=”7 December 2017″ format=”DHMS” link=false]

  • The workshop is designed for users who are new to real time quantitative PCR (qPCR), but all users are welcome. User will experience extracting RNA from blood and cells, performing RNA QC, converting RNA to cDNA, optimizing a qPCR reaction, assessing primers efficiency and determining expression level of their genes of interest. Clients will gain experience using DNA binding dyes chemistries. Lectures will include an overview of RNA extraction, gene expression, assay design and optimization, template preparation, analysis of results, additional applications of real-time PCR technology, and many more. Data analysis and simple statistical test will be included and will produce publication-ready figures. Some previous experience using standard PCR or real time PCR is helpful but not necessary for workshop attendees.

    OBJECTIVES

    1. To give participants a combination of lecture and hands-on laboratory experience using qPCR methods
    2. To expose and introduce a basic concept of qPCR and the analysis involved
    3. To provide the fundamentals of cDNA synthesis, quality controls in qPCR
    4. To promote awareness of application of qPCR in research

  • TimeDay 1Day 2
    9:00 amRegistration and breakfastHands-on:
    qPCR primers optimization (gradient qPCR)
    (MBU, level 4)
    10:00 amLecture:
    Introduction to molecular biology
    (Seminar room, level 5)
    11:00 amLecture:
    Introduction to relative quantification qPCR
    (Seminar room, level 5)
    Hands-on:
    qPCR standard curve analysis
    (MBU, level 4)
    11.30 am
    Hands-on:
    RNA extraction from blood and cells
    (MBU, level 4)
    1:00 pmLunch (Level 1)
    Lunch (Level 1)
    2:30 pmHands-on:
    - RNA QC using Nanodrop and Bioanalyzer
    - cDNA synthesis
    (MBU, level 4)
    Hands-on:
    Performing relative quantification qPCR on Biorad CFX96
    (MBU, level 4)
    3:30 pmData analysis of Relative quantification qPCR
    (Bioinformatics lab, level 3)
    4:30 pmDay 1 Review and TeaDay 2 Review and Tea

    *MBU (Makmal Biologi Umum)

  • UMBI

    1. Dr. Nurul Syakima Ab Mutalib
    2. Ryia Illani Mohd Yunos
    3. Sazuita Saidin
    4. Mohd Ridhwan Abdul Razak
    5. Norshahidah Mahamad Nazir
    6. Muhammad Nazirul ‘Azim Mohd Arpandi

     QIAGEN

    1. Dr. Cham Chun Wei

  • BANK INFORMATION

    AccountBendahari Universiti Kebangsaan Malaysia
    Account No86-0008114-0
    BankCIMB Berhad
    Bank AddressCIMB Cawangan Taman Maluri, No. 279 & 279A, Jalan Perkasa 1,
    Taman Maluri Off Jalan Cheras, 55100 WP Kuala Kumpur, Malaysia
    CPB/GST UKM0017 8225 9712
    Swift CodeCIBB MYKL

    Secretariat of ‘Real Time PCR Workshop: Real Time PCR for Gene Expression Analysis’
    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185

    For any inquiry, please directly contact Dr. Nurul Syakima (syakima@ppukm.ukm.edu.my) / Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)

  • The Real Time PCR For Gene Expression Analysis Workshop will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.

    For more information about the venue, please visit the UMBI website at https://www.ukm.my/umbi/.

    Please take note that the accommodation is NOT included in the registration fee. Kindly refer to the Accommodation tab for further information.

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html
    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292

  • Hotel Nearby UMBI:

    1. Hotel Sri Permaisuri Cheras (3-star hotel)
    2. Hotel EST Kuala Lumpur
    3. International Youth Centre (IYC) Residence

    Low Budget hotel are also available nearby the UMBI. Please refer to any hotel booking website for further information.

    **Important Note : KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

  • Dr. Nurul Syakima (syakima@ppukm.ukm.edu.my) / Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)
    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html
    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292