UKM Medical Molecular Biology Institute

 Leading Institute in Molecular Medicine

Flow Cytometry Lab

BD FACS Aria II Flow cytometer

The FACSAria II from Becton Dickinson (BD Biocsiences, San Jose, USA) is a high speed cell sorter for measuring and sorting fluorescently labelled cells. These cells can be prokaryotic (e.g. bacteria) or eukaryotic (e.g. mammalian) with a minimum size of 0.2 um (maximum 100 um). Fluorescence staining is usually performed with antibodies specific to the proteins of interest, which can be intra-cellular or on the cell surface. These primary antibodies are conjugated to a fluorescent dye, or a fluorescent secondary antibody may be used. Fluorescent substrates or compounds can also be detected.

A laser excites the fluorescent dyes bound to the cells. The emitted light has a longer wavelength and passes through a bandpass filter to shut out the excitatory and other unwanted light. The strength of this fluorescent light is measured with a detector (PMT) for each cell and is displayed in form of a histogram.


BD FACSVerse Flow cytometer

The BD FACSVerse™ flow cytometer was engineered from the ground up to offer remarkable performance, flexibility, and ease of operation for research applications using up to 10 parameters.