Workshop Outline
Liquid Chromatography – High Resolution Mass Spectroscopy (LC-HRMS) continued to grow in the last two decades as an analytical technique used in metabolomics. Additionally, by combining spectral resolution, mass accuracy, and mass fragmentation, MS metabolite identification capabilities have been enhanced and recognized as the most employed technique in metabolomics.
We will cover sample preparation (extraction with hands-on), data acquisition (qualitative and quantitative analysis), and finally data analysis using univariate and multivariate approaches in this two-day workshop.
Who should attend
Those who are interested in metabolomics and its applications (research scientists, academicians, postdoctoral fellows, and students) are encouraged to attend the workshop.
List of Speakers and Trainers
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As a pioneer in Metabolomics field in Malaysia, Professor Dr. Syarul Nataqain Baharum research is focused on the new insight of analytical and biological perspectives of the metabolomics in the field of systems biology, in particularly on the understanding of secondary metabolite production in local herbal plants as well as fluxomics studies. She has expanding her research focus in understanding host-pathogen interaction in aquaculture disease and also green therapeutic mechanism in neurogenerative diseases. In metabolomics research, pipeline of analytical tools such as LCMS, GCMS, CEMS, FTIR and NMR combined with Principal Component Analysis (PCA) is essential for the dynamic high throughput analysis. This frontier analysis which includes mathematical modelling has provided glimpse of cellular biology and fingerprint of functional network in the model organism in her lab. Currently she has published about 100 research articles, 4 books and 6 chapters in books. As project leader, she also received more than RM 4 million research grants in total amount. Under her supervision, 9 PhD students and 17 MSc were graduated. At the moment, she is supervising a total of 21 PhD students and 18 MSc students. She also has filed two patents and granted one Trade Mark (TM) and one Trade Secret. Her work has been awarded prestigious awards including BioInnovation Awards in 2011 and Selangor My Innovation Award, 2014. She also engaged in collaborative partnership with industries such as Medika Natura Sdn Bhd, AAR Sdn Bhd and FGV Sdn Bhd.
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If you are interested to collaborate with her, or be part of her research group, please drop her an email to nataqain@ukm.edu.my.
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Dr. Nurkhalida Kamal completed her PhD in Pharmaceutical Sciences (Natural Products Chemistry & Metabolomics) from the University of Strathclyde, Glasgow, UK in 2015. She then worked as a Postdoctoral Fellow at the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) focusing on metabolomics analysis of marine organisms including seaweed and marine microbes. She joined INBIOSIS in 2020 as a Research Fellow.
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Her research focuses on the application of metabolomics in natural products and food chemistry. She wants to understand the chemistry of various resources, why it is produced and what we can use it for. She has vast experiences in natural products isolation and elucidation from various organisms (plants, microbes, marine organisms and mushrooms), NMR and LCMS and chemometric data analysis. Her on-going research is to unravel the mechanism of immunomodulation of polyphenols-rich tempeh fraction on Caco-2 and HT-29 cell lines. Her interest also includes on using metabolomics as a tool to identify and biotechnologically optimize the production of bioactive secondary metabolites in medicinal plants, mushrooms, and microorganisms.
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Please feel free to visit her research group website (www.nurkhalidakamal.com) or contact Dr. Nurkhalida Kamal (nurkhalida.kamal@ukm.edu.my) for research and industrial projects collaboration.
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Dr Adlin Afzan has been a chemist researcher at the Herbal Medicine Research Centre, Institute for Medical Research, since 2004. She holds a PhD in Pharmaceutical Science from the University of Geneva, Switzerland. Her specialization in MS metabolomics and molecular networking-based metabolite annotations strategy has led her to manage several research projects on developing quality control methods for Malaysian medicinal plants and discovering bioactive natural products as anti-microbial, anti-proliferative and anti-depression. She is also a member of technical committees related to herbal medicine and pharmacy and serves as the associate editor and guest editors for International journals. Adlin is an advocate for continuous learning and enjoys sharing her voice delivering various training sessions and workshops that promote metabolomics and molecular networking with the natural product community.
Programme
6th August 2024 (Tuesday) - (Introduction)
Venue : Bilik Seminar IVI, Akademi Siber Teknopolis
| Time | Activity | Trainer |
| 8.30 am | Registration | Pn. Rafidah Ahmad, Pn. Sarah Ibrahim |
| 9.00 am | Opening ceremony and introduction | Prof. Dr. Syarul Nataqain Baharum |
| 9.10 am | Lecture 1: Fundamental of Metabolomics, Experimental design and quality control in Metabolomics | Prof. Dr. Syarul Nataqain Baharum |
| 10.30 am | Tea break | - |
| 11.00 am | Hands-on 1 LC-MS: Data Acquisition and Data Processing using MZMine | ChM. Dr. Khalida Kamal |
| 12.30 pm | Lunch break | - |
| 2.00 pm | Hands-on 2 LC-MS: Data Acquisition and Data Processing using SIRIUS | ChM. Dr. Khalida Kamal |
| 4.o0 pm | Afternoon break | - |
| 4.15 pm | Introduction to CODA
LC-TOF-MS service@INBIOSIS |
Pn. Rafidah Ahmad
Pn. Sarah Ibrahim |
| 5.00 pm | END OF DAY 1 |
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7th August 2024 (Wednesday) - (LC-MS)
Venue : Bilik Seminar IVI, Akademi Siber Teknopolis
| Time | Activity | Trainer |
| 9.00 am | Introduction to Day 2 | - |
| 9.10 am | Hands-on 1 Molecular Networking using GNPS | Dr. Adlin Afzan |
| 10.30 am | Tea break | - |
| 11.00 am | Hands-on 2 Molecular Networking using Cytoscape | Dr. Adlin Afzan |
| 12.30 pm | Lunch break | - |
| 2.00 pm | Hands-on 3 Molecular Netwoking using Cytoscape | Dr. Adlin Afzan |
| 4.o0 pm | Afternoon break | - |
| 4.15 pm | Summary, discussions and wrap-up | Dr. Adlin Afzan |
| 5.00 pm | Presentation of attendance certificate
END OF DAY 2 |
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