Institut Biologi Sistem

 Institute of Systems Biology

The Institute of Systems Biology (INBIOSIS) will organize Pathway Construction and Network Analysis Towards New Knowledge Discovery Workshop, for postgraduate students and researchers from ICGEB member states. It will be held from 20 - 22 August 2024 in Bangi Resort Hotel, generously funded by the ICGEB Training Grant. More information about this Course can be found here https://www.icgeb.org/new-knowledge-discovery-malaysia-2024/ .

Workshop Outline

This introductory workshop will focus on:

  • Construction of protein-protein interaction (PPI) using relevant databases i.e., STRING and GeneMANIA

  • Mapping of identified genes onto KEGG pathways

  • Construction of gene co-expression network using R package for gene co-expression network construction (WGCNA)

  • Functional annotation of genes/ proteins

  • Gene ontology (GO) enrichment of genes/ proteins

  • Pathway analysis and visualization

Upon the completion of this workshop, participants will be able to:

  • Construct pathways and PPI network from the list of genes/ proteins

  • Construct gene co-expression network using WGCNA

  • Perform GO and pathway analysis on the constructed pathway and PPI network

  • Understand a complete workflow of data acquisition, pathway construction, pathway analysis, and data interpretation.

Who should attend

Anyone interested in pathway analysis and its applications (researchers, academicians, and students), especially biologists from beginner to advanced levels.

List of Speakers and Trainers

  • Dr. Sarahani Harun is currently a research fellow at the Institute of Systems Biology (INBIOSIS). She graduated from the Universiti Kebangsaan Malaysia where she earned a Bachelor in Bioinformatics, Master in Molecular Biology and PhD in Systems Biology. Her research interest involves computational biology that includes pathway analysis in secondary metabolites, i.e., glucosinolates and cyanogenic glycosides to unravel potential genes in the respective biosynthetic pathways. This computational pipeline can be used for new gene discovery to establish genetic resources for crop improvement.

  • She is also interested in analyzing the transcriptome data generated from the oil pest, bagworm (Metisa plana) which is a collaborative project with Felda Global Ventures R&D. The identification of the key regulatory genes from the study would facilitate in controlling the pest infestation in oil palm. She is currently the coordinator for the Centre of Omics Data Analysis (CODA) (https://www.ukm.my/inbiosis/en/coda/). CODA is a one-stop solution research centre for omics data analysis. This centre provides consultation and assistance on high-throughput data analysis that is associated with systems biology. Dr. Sarahani can be contacted via email; sarahani@ukm.edu.my.

  • Ts. Dr. Nor Azlan currently is the Head of Centre for Bioinformatics Research, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia. He joined INBIOSIS as a research fellow in October 2015. His expertise is in bioinformatics and computational systems biology. His research focus is on the application of omics technologies to unravel new genes and pathways for the development of precision biotechnology. His current main organisms of interests are insect pests, including bagworm (Metisa plana), red palm weevil (Rhynchophorus ferrugineus) and cocoa pod borer (Conopomorpha cramerella). The long-term aim is to analyse the genome, transcriptome, proteome and metabolome of these species and use omics integration techniques to develop new methods of controlling them.  Expressions of interest for research collaboration and activity with Ts. Dr. Nor Azlan can be expressed via his email, norazlannm@ukm.edu.my.

  • Prof. Dr. Zeti Azura Mohamed Hussein is a Professor in Bioinformatics at the Department of Applied Physics, Faculty Science and Technology. She investigates the relationships between disease genes and disease networks using gene network analysis, pathway analysis and network analysis to understand disease pathobiology in human and plants alike which can’t be fully annotated using a single type of data thus the inclusion of big data analysis and omics data integration. Prof. Zeti’s research demonstrates how integrative analysis on omics data can be used to unravel new important knowledge and provide answers on the biological problems of interest. If you are interested to collaborate with her, or be part of her research group, please drop her an email to zeti.hussein@ukm.edu.my or visit her website at https://zetibioinfolab.wordpress.com

Programme

20th August 2024 (Tuesday)

Time Activity
9.00 am Introduction
9.10 am Welcome Speech
9.30 am Lecture 1: Introduction to pathway and network biology 
11.00 am Practical 1: Data preparation using omics datasets 
12.30 pm Lunch break
2.00 pm Practical 2: Leveraging pathway databases and tools (KEGG and Reactome)
4.00 pm Summary, discussions and wrap-up
4.30 pm END OF DAY 1

 

21st August 2024 (Wednesday)

Time Activity
9.00 am Lecture 2: Introduction to pathway databases/ tools and data integration Introduction to Cytoscape and more pathway databases 
10.30 am Tea break
11.00 am Practical 3: Cytoscape – pathway construction (STRING, GeneMANIA) 
12.30 pm Lunch break
2.00 pm Practical 4: Cytoscape – editing pathways, extending pathways/ networks (merging pathways/ networks) 
4.00 pm Summary, discussions and wrap-up
4.30 pm END OF DAY 2

 

22nd August 2024 (Thursday)

Time Activity
9.00 am Lecture 3: Network analysis
9.30 am Tea Break
10.00 am Practical 5: g:Profiler, DAVID tool – detect over-representation datasets 
12.30 pm Lunch break
2.00 pm Practical 6 : Cytoscape – GO enrichment/ KEGG pathway analysis
4.30 pm
  • Summary, discussions and wrap-up
  • Presentation of attendance certificate
4.45 pm END OF WORKSHOP

 

Register now or contact us for more details