Workshop Outline
Protein is one of the most important macromolecules for the formation and functionality of an organism. Protein functionality is closely related to the unique 3D structure of each of these macromolecules. Since the 1950s, the determination and understanding of the 3D structure of proteins has advanced human knowledge of biochemistry and biology to the atomic level. Various methods using X-ray, NMR, Cryo-Electronmicroscopy (Cryo-EM) and computing have been developed to determine high-quality 3D structures of proteins. Various databases and software related to 3D structure analysis have also been developed to aid the understanding of protein function guided by 3D structure. Considering that the 3D structure of proteins is being studied more and more, especially with the availability of 3D structure prediction software based on AI (artificial intelligence), therefore, the organization of this training focusing on the basic knowledge of 3D structure and understanding of protein function based on 3D structure is very necessary and is expected to receive a great response. encouraging.
Who should attend
Postgraduate students (Master and PhD students), Post Doctorate, Academicians and Researchers (Government and non-government institution)
List of Speakers and Trainers
Assoc. Prof. Dr. Ng Chyan Leong is a structural biologist. He completed his PhD in York Structural Biology Laboratory (YSBL), Chemistry Department, University of York, UK in 2007 under the supervision of Prof. Dr. Fred Antson. He then worked as a Postdoctoral Fellow in Dr. Venki Ramakrishnan’s group at the Medical Research Council, Laboratory of Molecular Biology (MRC-LMB), Cambridge, UK, focusing on ribosome and protein translation. He joined INBIOSIS in 2012 as a Research Fellow and has established the recombinant protein purification, protein crystallization and protein analysis platform in the Institute. Please feel free to contact Dr. Ng (clng@ukm.edu.my) for research and industrial projects collaboration.
Ts. Dr. Nor Azlan currently is the Head of Centre for Bioinformatics Research, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia. He joined INBIOSIS as a research fellow in October 2015. His expertise is in bioinformatics and computational systems biology. His research focus is on the application of omics technologies to unravel new genes and pathways for the development of precision biotechnology. His current main organisms of interests are insect pests, including bagworm (Metisa plana), red palm weevil (Rhynchophorus ferrugineus) and cocoa pod borer (Conopomorpha cramerella). The long-term aim is to analyse the genome, transcriptome, proteome and metabolome of these species and use omics integration techniques to develop new methods of controlling them. Expressions of interest for research collaboration and activity with Ts. Dr. Nor Azlan can be expressed via his email, norazlannm@ukm.edu.my.
Programme
22nd October 2024 (Tuesday)
| Time | Activity | Speaker | Venue |
| 8.30 am | Registration | Foyer | |
| 9.00 am | Introduction | Seminar Hall | |
| 9.10 am | Lecture 1: Introduction to Protein 3D structure - General (part 1) | Ts. Dr. Nor Azlan Nor Muhammad | Seminar Hall |
| 10.30 am | Morning break | Foyer | |
| 11.00 am | Lecture 2: Introduction to Protein Protein 3D structure – Methods of 3D structure determination (part 2) | Assoc. Prof. Dr. Ng Chyan Leong | Seminar Hall |
| 12.30 pm | Lunch break | ||
| 2.00 pm | Hands-on 1 – Introduction to Protein Data Bank (PDB), PDB file and 3D Structure visulisation programs (Chimera X, PyMOL, COOT) | Ts. Dr. Nor Azlan Nor Muhammad | Meeting Room |
| 3.30 pm | Tea break | Lobby | |
| 3.45 pm | Hands-on 2 Case study 1 Stucture prediction of a hypothetical protein using AlphaFold2 |
Assoc. Prof. Dr. Ng Chyan Leong | Meeting Room |
| 5.00 pm | END OF DAY 1 | Foyer |
23rd October 2024 (Wednesday)
| Time | Activity | Speaker | Venue |
| 9.00 am | Introduction | Seminar Hall | |
| 9.10 am | Lecture 3: 3D Protein Structure analysis (DALI, FOLDSEEK, Electrostatic Analysis) | Assoc. Prof. Dr. Ng Chyan Leong | Seminar Hall |
| 10.30 am | Morning break | Lobby | |
| 11.00 am | Lecture 4: 3D Structure Guided Mutagenesis | Seminar Hall | |
| 12.30 pm | Lunch break | Lobby | |
| 2.00 pm | Hands-on 3 3D Protein Structure analysis (Ramachandran plot, RMSD, superimposition and etc. ) |
Assoc. Prof. Dr. Ng Chyan Leong Ts. Dr. Nor Azlan Nor Muhammad |
Meeting Room |
| 4.00 pm | Tea break | Lobby | |
| 4.15 pm | Summary, discussions and wrap-up | Meeting Room | |
| 5.00 pm | Presentation of attendance certificate | Meeting Room |